High-throughput analysis of fatty acid composition of plasma glycerophospholipids

Glaser, Claudia; Demmelmair, Hans; Koletzko, Berthold

doi:10.1194/jlr.d000547

Cited by 83 publications

(83 citation statements)

References 31 publications

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“…Lipids, another class of metabolites associated with obesity in some publications, can be determined as a molecular species or through the FA composition of single lipid classes such as glycerophopholipids [30,31] , NEFA [32] , and acylglycerols [33] , as well as that of all lipid classes (total FA; tFA) [34] . Thus, comparison of the results of different reports is challenging.…”

Section: Lipidsmentioning

confidence: 99%

Metabolomic Biomarkers for Obesity in Humans: A Short Review

Rauschert¹,

Uhl²,

Koletzko³

et al. 2014

Ann Nutr Metab

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109

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Section: Lipidsmentioning

confidence: 99%

Metabolomic Biomarkers for Obesity in Humans: A Short Review

Rauschert¹,

Uhl²,

Koletzko³

et al. 2014

Ann Nutr Metab

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109

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“…To analyze FA concentrations, EDTA plasma samples were picked and transported to the Division of Metabolic Diseases and Nutritional Medicine, Dr. von Hauner Children's Hospital, University of Munich Medical Center, in 2010. After being thawed, the analysis of plasma glycerophospholipid FA (hereafter referred to as "plasma FA") composition was performed by a sensitive and precise high-throughput method described recently (24). The average coefficient of variation was 15.7%.…”

Section: Fatty Acid Analysesmentioning

confidence: 99%

Maternal LC-PUFA status during pregnancy and child problem behavior: the Generation R Study

et al. 2014

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Background: Omega 3 (n-3) and 6 (n-6) long-chain polyunsaturated fatty acids (LC-PUFAs) and the n-3:n-6 ratio are important for brain development. Whether maternal LC-PUFA status during pregnancy affects risk of problem behavior in later childhood is unclear. Methods: Within a population-based cohort, we measured maternal plasma docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), and arachidonic acid (AA) concentrations and n-3:n-6-ratio in mid-pregnancy. Child emotional and behavioral problems at 6 y of age were assessed by parents (child behavior checklist), teachers (teacher report form), and combined parent/teacher report. results: Higher maternal DHA and n-3:n-6 ratio were associated with fewer child emotional problems using parent (odds ratio (OR) DHA = 0.82; 95% confidence interval (CI): 0.70, 0.96; P = 0.02 and OR n-3:n-6 = 0.83; 95% CI: 0.71, 0.96; P = 0.01; n = 5,307) and combined parent/teacher scores (OR DHA = 0.79; 95% CI: 0.66, 0.95; P = 0.01 and OR n-3:n-6 = 0.77; 95% CI: 0.65, 0.92; P < 0.01; n = 2,828). Higher AA was associated with more child behavioral problems using teacher (OR = 1.10; 95% CI: 1.00, 1.20; P = 0.04; n = 3,365) and combined parent/teacher scores (OR = 1.12; 95% CI: 1.02, 1.22; P = 0.02; n = 2,827). Maternal EPA was not associated with child problem behavior. conclusion: Indications of associations of maternal LC-PUFA status with child emotional and behavioral problems were found. Future research is needed to identify LC-PUFAsensitive periods of fetal brain development by including multiple assessments of prenatal LC-PUFA status. t wo families of polyunsaturated fatty acids (PUFAs), the omega 3 (n-3) and 6 (n-6) fatty acids (FAs), are nutrients that play a major role in brain development and functioning. They affect numerous processes, including membrane fluidity, neurotransmission, and gene expression (1). The long-chain PUFAs (LC-PUFAs), eicosapentaenoic acid (EPA; C20:5n-3), docosahexaenoic acid (DHA; C22:6n-3), and arachidonic acid (AA; C20:4n-6) are the main FAs that are metabolically active.In addition, the ratio between n-3 and n-6 FAs is important for optimal brain functioning. During the last century, however, the Western diet has provided a lower n-3 and higher n-6 intake than that in previous generations, diminishing the n-3:n-6 ratio (2).Because of their role in brain function and development, maternal LC-PUFAs during fetal development have been hypothesized to affect child problem behavior. Approximately half of the dry weight of the brain is lipid, of which 35% are PUFA, most of which is LC-PUFA (1). Increase in AA and DHA content of brain tissue starts during the third trimester of pregnancy (3), indicating that the fetal period possibly is a sensitive period. Moreover, the fetus mostly depends on maternal LC-PUFA supply for its development, because fetal LC-PUFA synthesis is limited (4).Research in this field is however scarce, and results are conflicting. Two studies on maternal fish intake, a major source of DHA, during pregnancy and offspring problem beha...

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“…Samples were centrifuged, and serum was frozen in plastic vials and stored at 280°C until analyzed. The analysis of serum glycerophospholipid fatty acid composition was performed by a sensitive and precise high-throughput method described recently (26). In brief, 100 lL serum, 100 lL internal standard (1,2-dipentadecanoyl-sn-glycero-3-phosphocholine dissolved in methanol), and 0.6 mL methanol (precooled to 5°C) were combined in glass tubes and shaken for 30 s. Samples were centrifuged and the supernatant fluid containing phospholipids and nonesterified fatty acids were transferred into additional glass tubes.…”

Section: Fatty Acid Analyses In Cord Blood Serummentioning

confidence: 99%

Effect of fatty acid status in cord blood serum on children's behavioral difficulties at 10 y of age: results from the LISAplus Study

Kohlboeck¹,

Glaser²,

Tiesler³

et al. 2011

The American Journal of Clinical Nutrition

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Background: Little is known about the effect of fatty acid (FA) concentrations in cord blood on long-term behavioral outcomes. Objective: We assessed the effect of FAs in cord blood serum on children's behavioral difficulties at the age of 10 y. Design: A longitudinal study of 416 children from the populationbased Influences of Lifestyle-Related Factors on the Immune System and the Development of Allergies in Childhood (LISAplus) birth cohort from Munich was conducted. Individual glycerophospholipid FAs in blood were analyzed in venous cord blood. Data on children's behavior were collected with a parent-reported Strength and Difficulties Questionnaire at 10 y of age. Zero-inflated Poisson regression models were applied and adjusted for sex, parental income, smoking during pregnancy, and dietary intake of arachidonic acid (AA) and DHA at 10 y. Conclusion: Increased concentrations of DHA, LC-PUFAs, and AA in cord blood serum were associated with lower scores on a parentcompleted behavioral screen. An appropriate FA supply to the developing fetus may be essential for optimal long-term behavioral outcomes in children.Am J Clin Nutr 2011;94:1592-9.

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High-throughput analysis of fatty acid composition of plasma glycerophospholipids

Cited by 83 publications

References 31 publications

Metabolomic Biomarkers for Obesity in Humans: A Short Review

Metabolomic Biomarkers for Obesity in Humans: A Short Review

Maternal LC-PUFA status during pregnancy and child problem behavior: the Generation R Study

Effect of fatty acid status in cord blood serum on children's behavioral difficulties at 10 y of age: results from the LISAplus Study

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