High tetragonula sp honey addition reduce cell proliferation on fibroblast preputium culture

Shafira, M; Pramono, Andri; Nugrohowati, Nunuk; Sahlan, Muhamad

doi:10.1088/1757-899x/508/1/012146

Cited by 5 publications

(3 citation statements)

References 10 publications

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“…Honey is typically consumed directly and has many uses in traditional medicine and functional food ( Meo et al, 2017a , Meo et al, 2017b , Sahlan et al, 2019 ) . For example, honey has been used to promote wound healing ( Jull et al, 2008 ), has antimicrobial activities ( Lusby et al, 2005 ), regulates food absorption in the intestine ( Nemoseck et al, 2011 ), has anti-inflammatory and analgesic activities ( Alzubier and Okechukwu, 2011 ), antibacterial and antioxidant activities ( Sahlan et al, 2019 ), reduce cell proliferation ( Shafira et al, 2019 ) and enhancement of overall sperm quality ( Fakhrildin and Alsaadi, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

The Effects of stingless bee (Tetragonula biroi) honey on streptozotocin-induced diabetes mellitus in rats

Sahlan

Rahmawati

Pratami

et al. 2020

Saudi Journal of Biological Sciences

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Diabetes mellitus (DM) is a metabolic disease characterised by chronic hyperglycaemia with impaired carbohydrate, fat and protein metabolism caused by defects in insulin secretion or action. Based on our previous research, stingless bee honey (SLBH) from Tetragonula biroi and T. laeviceps can inhibit alpha-glucosidase activities. Therefore, the aim of the present study was to determine the effects of daily oral administration of SLBH on body weight (BW) and fasting blood glucose (FBG) levels of male rats with streptozotocin (STZ)-induced DM. Thirty-six male Sprague Dawley rats were divided into six groups of six rats each. One group of normal non-diabetic rats served as a positive control. The diabetic groups were intraperitoneally (i.p.) injected with STZ (50 mg/kg BW) for induction of DM and divided into five equal subgroups of six animals each: an untreated group as a negative control; a group treated with 0.6 mg/kg BW of glibenclamide as a positive control and three SLBN treatment groups that had daily oral administration of 0.5, 1.0 or 2.0 g/kg BW, respectively, for 35 days. The results showed that SLBH significantly reduced loss of BW in diabetic rats. FBG levels in diabetic rat blood, collected from the tail, were measured using Accu-Chek test strips. The FBG levels in diabetic rats that have oral administered intake with glibenclamide and SLBH were stable. There were no changes in serum FBG levels in SLBH-treated diabetic rats for 35 days. Pancreatic histopathology results from all groups showed no abnormalities or tissue damage in either diabetic or non-diabetic rats. The results of this study show that administration of SLBH reduced BW loss or improved BW of rats with STZ-induced DM. Meanwhile, the reduction in loss of BW that occurred in diabetic rats after 35 days of SLBH administration was the result of reduced formation of fats and proteins, which are broken down into energy. Further research is needed to determine the antidiabetic effects of honey from other stingless honeybee species .

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Section: Introductionmentioning

confidence: 99%

The Effects of stingless bee (Tetragonula biroi) honey on streptozotocin-induced diabetes mellitus in rats

Sahlan

Rahmawati

Pratami

et al. 2020

Saudi Journal of Biological Sciences

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“…From our previous research, this honeybee product is known having a high degree of anti-inflammatory, antiangionic, antioxidant and antibacterial activity, anticancer, antidiabetes (Diva et al, 2019;Farida et al, 2019;Iqbal et al, 2019;Miyata et al, 2019). Bioactive compounds which is presumed gained from Kelulut honey have gained more interest recently in our previous research (Rahmawati et al, 2019;Sahlan et al, 2019;Shafira et al, 2019). The publication of kelulut bees has increased over the past 10 years from 288 to 1870 publications in 2018 (Avila et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Purification and characterization of proteins in multifloral honey from kelulut bee (stingless bee)

Sahlan

Mahira

Wiratama

et al. 2019

Heliyon

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The kelulut bee (Meliponini) is a subfamily of stingless bees that produce honey. A total of 89 species out of a total of 500 species of kelulut bees are known to originate from the Indo-Australian region. Kelulut bees do not have quality standards so they still refer to the Codex and EU Directive which basically only applied for Apis honey. The Codex and EU Directive are formed by several psychochemical parameters, one of it is diastase activity. Diastase activity in kelulut honey is known not to meet existing standards or even undetectable. Therefore, this study aimed to explore proteins inside kelulut honey and investigate the possibility of using a specific protein as a biomarker to differentiate honey produced by kelulut bee from other honey. This research can also be considered as an initial step to optimize the exploration of protein in kelulut honey. This research is divided into two sections which are the preliminary research and the research expansion. From preliminary section, glucose dehydrogenase enzyme (GDH) was found to be present inside Tetragonula spp honey. A further examination of GDH enzyme was made in four kelulut bee honeys namely Tetragonula leaviceps, T. biroi, Heterotrigona itama, and Geniotrigona thoracica. The preliminary research has five stages that are exactly the as expansion research section except it didn't include GDH activity measurement. The research includes seven main stages. First honeys were dialyzed to remove the sugar content followed by centrifugation. The samples were then purified using liquid chromatography with anion exchanger column. The molecular weight of proteins was analysed by SDS-PAGE method. The GDH activity was measured using spectrophotometer followed by qualitative analysis using LC-MS/MS. The peptide sequences resulted from LC-MS/MS were then matched with Uniprot to identify the unknow protein. The results showed that only T. biroi and T. laeviceps had GDH enzyme activity of 0,1891 U/mL and 0,1652–1,579 U/mL, respectively. Bands from both species were also qualitatively identified as GDH. With these results, it can be concluded that the GDH enzyme cannot be used as a biomarker to distinguish the kelulut honey.

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“…However, studies have reported honey is safe for consumption by diabetic patients, considering its low glycaemic index and overall effect on glycaemic control ( Kadirvelu and Gurtu, 2013 ; Bobiş et al, 2018 ). The capabilities of honey to regulate intestinal food metabolism, antioxidant and anti-inflammatory activities, and to reduce blood glucose levels have been reported ( Nemoseck et al, 2011 ; Sahlan et al, 2019 ; Shafira et al, 2019 ; Sahlan et al, 2020 ). However, information is lacking on the use of plantain bulb or corm extracts, either in their pure or blended forms, with beverages.…”

Section: Introductionmentioning

confidence: 99%

Effect of Plantain Bulb’s Extract-Beverage Blend on Blood Glucose Levels, Antioxidant Status, and Carbohydrate Hydrolysing Enzymes in Streptozotocin-Induced Diabetic Rats

Olagunju¹,

Oluwajuyitan²,

Oyeleye³

2020

pnf

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High tetragonula sp honey addition reduce cell proliferation on fibroblast preputium culture

Cited by 5 publications

References 10 publications

The Effects of stingless bee (Tetragonula biroi) honey on streptozotocin-induced diabetes mellitus in rats

The Effects of stingless bee (Tetragonula biroi) honey on streptozotocin-induced diabetes mellitus in rats

Purification and characterization of proteins in multifloral honey from kelulut bee (stingless bee)

Effect of Plantain Bulb’s Extract-Beverage Blend on Blood Glucose Levels, Antioxidant Status, and Carbohydrate Hydrolysing Enzymes in Streptozotocin-Induced Diabetic Rats

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