High temperature and temperature programming in capillary electrochromatography

Djordjevic, Nebojša M.; Fitzpatrick, Fiona; Houdiere, Fabrice; Lerch, Guido; Rozing, Gerard P.

doi:10.1016/s0021-9673(99)01129-2

Cited by 37 publications

(26 citation statements)

References 28 publications

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“…Hexaldehyde hydrazone was eluted ahead of p-tolualdehyde hydrazone at lower temperature (10-157C); the two solutes were coeluted at moderate temperature (20-357C); however, the elution order was reversed at higher temperature (40-457C). Such a temperature effect on selectivity was also observed in HPLC [31], MEKC [32,33], and CEC [34]. In general, this kind of temperature dependence of selectivity is not common for uncharged solutes in both HPLC [35] and MEKC [36], but particularly useful for improvement of separation and reversal of elution order.…”

Section: Effect Of Temperaturementioning

confidence: 81%

Separation of 4-dimethylamino-6-(4-methoxy-1-naphthyl)-1,3,5-triazine-2-hydrazine derivatives of carbonyl compounds by reversed-phase capillary electrochromatography

Liu

Zou

2001

Electrophoresis

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4-Dimethylamino-6-(4-methoxy-1-naphthyl)-1,3,5-triazine-2-hydrazine (DMNTH) is a novel derivatizing reagent specially designed for the determination of carbonyl compounds. In this work, we describe the separation of DMNTH-derivatized carbonyl compounds by reversed-phase capillary electrochromatography (CEC). After systematic investigations of the effects of experimental conditions viz. pH and concentration of buffer, type of stationary phase, injection volume of sample, organic modifier, and temperature, optimal conditions were found. The sample compounds, which were separated with gradient high performance liquid chromatography (HPLC), were separated by CEC under isocratic elution due to the high efficiency. Comparisons of separations by CEC and micellar electrokinetic chromatography (MEKC) were made.

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Section: Effect Of Temperaturementioning

confidence: 81%

Separation of 4-dimethylamino-6-(4-methoxy-1-naphthyl)-1,3,5-triazine-2-hydrazine derivatives of carbonyl compounds by reversed-phase capillary electrochromatography

Liu

Zou

2001

Electrophoresis

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“…221 High-temperature CEC and temperature programming resulted in a considerable reduction of the separation run time of steroids, and the latter may be a good alternative to solvent programming. 222 Usually ODS phases are used for steroid separations but the successful use of macroporous, spherical polystyrene-divinylbenzene stationary phase was also described. 223 Of the practical applications the rapid analysis of norgestimate and its degradants, 224 tipredane and related impurities 225 and fluticasone propionate and related impurities 226 is mentioned.…”

Section: ·4 Capillary Electrochromatography (Cec) and Cec-msmentioning

confidence: 99%

Recent Advances in the Analysis of Steroid Hormones and Related Drugs

Gǒrög¹

2004

ANAL. SCI.

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“…At pH 4.7, the separation is mainly based on the different distribution ratios between stationary and mobile phase. It decreases with increasing temperature according to the Van't Hoff equation [31]:…”

Section: Influence Of Temperaturementioning

confidence: 99%

Separation of selected humic degradation compounds by capillary electrochromatography with monolithic and packed columns

Ping

Schmitt‐Kopplin²,

Hertkorn³

et al. 2003

Electrophoresis

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The separation of selected lignin/humic substance (HS) degradation compounds by capillary electrochromatography (CEC) with a methacrylate-based monolithic column and a conventional column packed with 5 microm octadecyl silica (ODS) particles is presented. The effects of organic modifier concentration, pH of the mobile phase, ionic strength, applied voltage, and temperature on the separation were investigated to determine the optimal separation conditions. With the increase of pH in the mobile phase, some of analytes start to ionize and both chromatographic partition and electrophoresis can play roles in separation simultaneously. Accordingly, different selectivity from high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE) could be achieved. The performances of both kinds of columns were compared. The results showed that the peaks of compounds obtained on the former column were much wider than those on the latter one, although good separation efficiency of alkylbenzenes could be readily achieved; the most probable reasons for this behavior and method to solve this problem were briefly discussed. The CEC of a soil fulvic acid with a monolithic column produced partly resolved broad bands; by means of nuclear magnetic resonance (NMR) analysis a wide range of oxygen derived aromatic substitution patterns was found with prominent contributions from phenolic and carboxylic groups.

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High temperature and temperature programming in capillary electrochromatography

Cited by 37 publications

References 28 publications

Separation of 4-dimethylamino-6-(4-methoxy-1-naphthyl)-1,3,5-triazine-2-hydrazine derivatives of carbonyl compounds by reversed-phase capillary electrochromatography

Separation of 4-dimethylamino-6-(4-methoxy-1-naphthyl)-1,3,5-triazine-2-hydrazine derivatives of carbonyl compounds by reversed-phase capillary electrochromatography

Recent Advances in the Analysis of Steroid Hormones and Related Drugs

Separation of selected humic degradation compounds by capillary electrochromatography with monolithic and packed columns

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