High Survivability of Cheese Whey-Grown
            <i>Rhizobium meliloti</i>
            Cells upon Exposure to Physical Stress

Bissonnette, Nathalie; Lalande, Roger

doi:10.1128/aem.54.1.183-187.1988

Cited by 15 publications

(2 citation statements)

References 19 publications

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“…The poor nodulation in the liquid treatments was more pronounced at Elbow in 1997 and at Outlook in 1998 (data not shown), which were drier than the other sites (Tables 1 and 3). Bissonnette and Lalande (1988) observed that carrier material for inoculum affected survival of rhizobia during stress. Zdor and Pueppke (1990), working with liquid and peat inoculant carriers, indicated that a peat formulation may help protect rhizobial strains from adverse environmental effects that would reduce their populations.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Rhizobial Inoculation Methods for Chickpea

2002

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treating the seed with a peat-based powder or liquid inoculant before planting. However, some studies have Rhizobia inoculated onto legume seeds are often exposed to adshown that a large majority of the rhizobia, applied to verse environmental conditions, which can affect survival and subsequent effectiveness. Hence, soil-applied granular inoculants have re-seed via conventional seed inoculation, die on the seed ceived much attention recently. We examined the efficacy of various before seeding or shortly after placement in the soil inoculation methods at four sites in Saskatchewan, Canada, in 1997 due to exposure to seed treatment chemicals, seed-coat and 1998 using desi-and kabuli-type chickpea (Cicer arietinum L.).

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Section: Resultsmentioning

confidence: 99%

Evaluation of Rhizobial Inoculation Methods for Chickpea

2002

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“…1985). After 2 d at -18" or -80°C Rhizobium meliloti cell suspensions in whey had almost 100% survival at thawing (Bissonnette and Lalande 1988).…”

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confidence: 99%

Frozen storage (—20° and —80°C) of soybean Bradyrhizobium cell suspensions

Allievi

Salardi

1993

Lett Appl Microbiol

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Liquid cultures of Bradyrhizobium japonicum were added in a 1:1 ratio to 20% aqueous skim milk, or centrifuged and the cells resuspended in 10% skim milk. The suspensions were stored at —20° or —80°C for 7 months and cell survival assessed. At —20°C, there was a decrease in the viable count of about two logs in liquid culture whilst for cells resuspended in 10% skim milk the decrease was limited to one log. The temperature of —80°C was found to be in itself protective and the surviving rhizobial cells maintained their infectivity and effectiveness. Thus appropriate freezing conditions provide a suitable method to store soybean rhizobia cells prior to preparing the legume inoculant.

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FT‐IR spectroscopic studies on molecular interactions of cryoprotectant agents with bacteria

Zeroual¹,

Millot²,

Choisy

et al. 1995

Biospectroscopy

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SYNOPSISFourier transform infrared spectroscopy was used to investigate, without any destructive interference, interactions of the cryoprotective agents, glycerol and dimethyl sulfoxide with Brudyrhizobium juponicum. The intracellular spectrum of glycerol, obtained by subtracting the spectrum of control bacteria from that of glycerol-treated bacteria, showed no differences in infrared features compared to that of pure glycerol. This was not the case when dimethyl sulfoxide treatment was used. The intracellular spectrum displayed important modifications compared to that of the pure chemical. Spectral analysis showed that glycerol and dimethyl sulfoxide uptake required only a few minutes. Moreover, the infrared features resulting from the presence of pure glycerol, in bacteria, were still existent for up to 2 or 3 hours after thawing process. The deconvoluted Amide I and Amide I1 bands of bacterial proteins reflected important changes in secondary structure after treatment of bacteria with dimethyl sulfoxide (increase in &sheet and loss of random coil content). In contrast to glycerol, the cryoprotective action of dimethyl sulfoxide probably involves interactions with a wide range of intrabacterial species.

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High Survivability of Cheese Whey-Grown Rhizobium meliloti Cells upon Exposure to Physical Stress

Cited by 15 publications

References 19 publications

Evaluation of Rhizobial Inoculation Methods for Chickpea

Evaluation of Rhizobial Inoculation Methods for Chickpea

Frozen storage (—20° and —80°C) of soybean Bradyrhizobium cell suspensions

FT‐IR spectroscopic studies on molecular interactions of cryoprotectant agents with bacteria

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