volume 16, issue 4, P196-198 1993
DOI: 10.1111/j.1472-765x.1993.tb01395.x
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Abstract: Liquid cultures of Bradyrhizobium japonicum were added in a 1:1 ratio to 20% aqueous skim milk, or centrifuged and the cells resuspended in 10% skim milk. The suspensions were stored at —20° or —80°C for 7 months and cell survival assessed. At —20°C, there was a decrease in the viable count of about two logs in liquid culture whilst for cells resuspended in 10% skim milk the decrease was limited to one log. The temperature of —80°C was found to be in itself protective and the surviving rhizobial cells maintai…

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