High Substrate Uptake Rates Empower Vibrio natriegens as Production Host for Industrial Biotechnology

Abstract

The productivity of industrial fermentation processes is essentially limited by the biomass specific substrate consumption rate (q) of the applied microbial production system. Since q depends on the growth rate (μ), we highlight the potential of the fastest growing non-pathogenic bacterium, , as novel candidate for future biotechnological processes. grows rapidly in BHIN complex medium with a μ of up to 4.43 h (doubling time of 9.4 min) as well as in minimal medium supplemented with various industrially relevant substrates. Bioreactor cultivations in minimal medium with glucose showed that possesses an exceptionally high q under aerobic (3.90 ± 0.08 g g h) and anaerobic (7.81 ± 0.71 g g h) conditions. Fermentations with resting cells of genetically engineered under anaerobic conditions yielded an overall volumetric productivity of 0.56 ± 0.10 g alanine L min (i.e. 34 g L h). These inherent properties render a promising new microbial platform for future industrial fermentation processes operating with high productivity. Low conversion rates are one major challenge to realize microbial fermentation processes for the production of commodities operating competitively to existing petrochemical approaches. For this reason, we screened for a novel platform organism possessing superior characteristics to traditionally employed microbial systems. We identified the fast growing which exhibits a versatile metabolism and shows striking growth and conversion rates, as a solid candidate to reach outstanding productivities. Due to these inherent characteristics can speed up common laboratory routines, is suitable for already existing production procedures, and forms an excellent foundation to engineer next generation bioprocesses.

Exploiting the Feedstock Flexibility of the Emergent Synthetic Biology Chassis Vibrio natriegens for Engineered Natural Product Production

“…In order to expand the capabilities of engineered organisms, synthetic biologists have begun exploring non-model organisms that display characteristics not found in model organisms such as Escherichia coli [1][2][3]. The type strain of Vibrio natriegens is a Gram-negative, facultatively anaerobic, salt marsh isolate [4], that has been put forward as an emergent synthetic biology chassis due in large part to its non-pathogenic nature and extremely fast growth rate (reported doubling time < 10 min) [5][6][7][8][9][10][11][12]. To put this in context, its growth rate using glucose is at least two times faster than some industrially-relevant microorganisms including E. coli, Bacillus subtilis, Corynebacterium glutamicum, and yeast [7].…”

A synthetic glycerol assimilation pathway demonstrates biochemical constraints of cellular metabolism

“…The supernatant was analysed by HPLC using an Agilent 1200 Series apparatus (Agilent, Santa Clara, CA, USA) with a refractive index detector equipped with a Rezex ROA organic acid column H + (8%) column (300 × 7.8 mm, 8 μm; Phenomenex, Torrance, CA, USA), protected by a Phenomenex security guard column carbo‐H (4 × 3.0 mm ID) (Phenomenex, Torrance, CA, USA) as described in Ref. . Dihydroxyacetone concentrations in supernatants of the bioreactor or from tube experiments were determined enzymatically according to Ref.…”

Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non‐traditional microorganisms

“…Further research on the central carbon metabolism of V. natriegens has been performed in glucose-grown cells using 13 C-based metabolic flux analysis, showing a flux distribution in central carbon metabolism similar to that of E. coli (Long et al, 2017). Its suitability to be grown in large bioreactor cultures using different carbon sources has been also tested, achieving cell densities around 20 g cell dry weight l À1 , and rates of substrate consumption faster than those of E. coli and S. cerevisiae have been reported under these growth conditions (Hoffart et al, 2017). V. natriegens has been successfully applied for the production of industrially relevant chemical compounds such as PHAs and L-alanine, hinting a great potential for biotechnological applications in the near future (Dalia et al, 2017;Hoffart et al, 2017).…”