High speed optical holography of retinal blood flow

Pellizzari, M.; Silva, Manuel; Dégardin, Julie; Sahel, José‐Alain; Fink, Mathias; Pâques, Michel; Atlan, Michaël

doi:10.1364/ol.41.003503

Cited by 15 publications

(11 citation statements)

References 28 publications

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“…Laser Doppler holography (LDH) is another optical Doppler imaging technique that can be described as the interferometric version of full-field LDF 34 , 35 . The most notable conceptual difference between LDH and LSCI/LDF and also Doppler ultrasound is that a reference arm is introduced to measure the frequency shift by optical mixing.…”

Section: Introductionmentioning

confidence: 99%

Retinal blood flow reversal quantitatively monitored in out-of-plane vessels with laser Doppler holography

Puyo

Pâques

Atlan

2021

Sci Rep

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Laser Doppler holography is a planar blood flow imaging technique recently introduced in ophthalmology to image human retinal and choroidal blood flow non-invasively. Here we present a digital method based on the Doppler spectrum asymmetry that reveals the local direction of blood flow with respect to the optical axis in out-of-plane vessels. This directional information is overlaid on standard grayscale blood flow images to depict flow moving towards the camera in red and flow moving away from the camera in blue, as in ultrasound color Doppler imaging. We show that thanks to the strong contribution of backscattering to the Doppler spectrum in out-of-plane vessels, the local axial direction of blood flow can be revealed with a high temporal resolution, which enables us to evidence pathological blood flow reversals. We also demonstrate the use of optical Doppler spectrograms to quantitatively monitor retinal blood flow reversals.

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Section: Introductionmentioning

confidence: 99%

Retinal blood flow reversal quantitatively monitored in out-of-plane vessels with laser Doppler holography

Puyo

Pâques

Atlan

2021

Sci Rep

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“…Laser Doppler holography (LDH) is a digital holographic method that relies on analyzing the beat frequency spectrum between a Doppler broadened beam and a monochromatic reference beam to extract a blood flow contrast [19][20][21]. We have shown in humans that LDH can reveal blood flow in retinal vessels over an extended field of view with a temporal resolution down to a few milliseconds [22].…”

Section: Introductionmentioning

confidence: 99%

Choroidal vasculature imaging with laser Doppler holography

Puyo¹,

Pâques²,

Fink³

et al. 2019

Biomed. Opt. Express

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The choroid is a highly vascularized tissue supplying the retinal pigment epithelium and photoreceptors. Its implication in retinal diseases is gaining increasing interest. However, investigating the anatomy and flow of the choroid remains challenging. Here we show that laser Doppler holography provides high-contrast imaging of choroidal vessels in humans, with a spatial resolution comparable to state-of-the-art indocyanine green angiography and optical coherence tomography. Additionally, laser Doppler holography contributes to sort out choroidal arteries and veins by using a power Doppler spectral analysis. We thus demonstrate the potential of laser Doppler holography to improve our understanding of the anatomy and flow of the choroidal vascular network.

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“…Thus so far flying spot and line-scanning LDF have not demonstrated hemodynamic measurements over a 2-D field of view [26,27]. Laser Doppler holography (LDH) is a digital holographic method that relies on measuring the DPSD from the interference between a Doppler broadened beam and a holographic reference beam, allowing to extract a blood flow contrast similar to LDF [28][29][30]. The additional reference beam is a plane or spherical wave and provides two advantages: it allows to use a higher camera frame rate by simply increasing the reference beam power, and it opens the door to all sorts of numerical manipulation of the wave field, such as numerical refocusing and digital adaptive optics [31,32], or synthetic subaperture Doppler measurements [33][34][35].…”

Section: Introductionmentioning

confidence: 99%

Waveform analysis of human retinal and choroidal blood flow with laser Doppler holography

Puyo¹,

Pâques²,

Fink³

et al. 2019

Biomed. Opt. Express

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Laser Doppler holography was introduced as a full-field imaging technique to measure blood flow in the retina and choroid with an as yet unrivaled temporal resolution. We here investigate separating the different contributions to the power Doppler signal in order to isolate the flow waveforms of vessels in the posterior pole of the human eye. Distinct flow behaviors are found in retinal arteries and veins with seemingly interrelated waveforms. We demonstrate a full field mapping of the local resistivity index, and the possibility to perform unambiguous identification of retinal arteries and veins on the basis of their systolodiastolic variations. Finally we investigate the arterial flow waveforms in the retina and choroid and find synchronous and similar waveforms, although with a lower pulsatility in choroidal arteries. This work demonstrates the potential held by laser Doppler holography to study ocular hemodynamics in healthy and diseased eyes.

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High speed optical holography of retinal blood flow

Cited by 15 publications

References 28 publications

Retinal blood flow reversal quantitatively monitored in out-of-plane vessels with laser Doppler holography

Retinal blood flow reversal quantitatively monitored in out-of-plane vessels with laser Doppler holography

Choroidal vasculature imaging with laser Doppler holography

Waveform analysis of human retinal and choroidal blood flow with laser Doppler holography

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