Choroidal vasculature imaging with laser Doppler holography

Puyo, Leo; Pâques, Michel; Fink, Mathias; Sahel, José‐Alain; Atlan, Michaël

doi:10.1364/boe.10.000995

Cited by 31 publications

(29 citation statements)

References 30 publications

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“…They confirmed the location of PCAs on laser doppler holography (LDH) ( Figure 3). 16 In-vivo, intrascleral vessels could also be localized using ICG angiography in eyes with choroidal atrophy. 10 In 66.9% of the eyes, it was localized in the foveal area, which is more than in our cohort.…”

Section: Discussionmentioning

confidence: 99%

Topographic Analysis of Short Posterior Ciliary Arteries Using Wide-Field Swept-Source En-Face Optical Coherence Tomography

Lejoyeux

Atia

Vupparaboina

et al. 2021

Preprint

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Purpose: To study the topographic distribution of the short posterior ciliary arteries (SPCA) entry sites into the choroid in normal eyes using structural en-face swept source optical coherence tomography (SS-OCT). Methods: Retrospective analysis of SS-OCT scans (wide-field structural SS-OCT 12x12mm) of 13 healthy subjects was performed. Cross-sectional swept-source OCT scans derived from a volume scan were represented as en-face image display following the Choroid-Scleral Interface to obtain en-face OCT. SPCAs in their last scleral location before choroidal entrance were identified manually, counted and localized by two masked observers. Correlations between two masked observers were analyzed using inter- and intra- class correlation. Results: Accuracy for the choroidal inner and outer border segmentation was 95-99%. Eighteen eyes from 13 normal subjects were included for SPCA analysis. The mean number of arteries was 13.8±3.5 per eye. Thirty-six percent were in the center of the posterior pole image; however, 21% were in the temporal part of the posterior pole. Median accuracy of the detection is 0.94. The correlation between the two observers was fair (0.54).Conclusion: Our algorithm allows visualization of the SPCA at the posterior pole of the eye using wide-field en-face SS-OCT. It can also help the clinicians to study the SPCAs in numerous ocular diseases, particularly its relationship with focal choroidal diseases.

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Section: Discussionmentioning

confidence: 99%

Topographic Analysis of Short Posterior Ciliary Arteries Using Wide-Field Swept-Source En-Face Optical Coherence Tomography

Lejoyeux

Atia

Vupparaboina

et al. 2021

Preprint

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“…Finally, in Fig. 9(e) and (f), we show the composite color images obtained by merging low and high frequency power Doppler images (calculated with the frequency ranges 2.5-6, and 10-30 kHz, respectively) in cyan and red, following the process we previously demonstrated [37].…”

Section: Arteriovenous Differentiation In the Retina And Choroidmentioning

confidence: 98%

“…In the choroid, the arteriovenous segregation relies on the ability to analyze the flow in choroidal vessels. Choroidal the Doppler broadening due to blood flow in choroidal veins lies in the frequency range 2.5-6 kHz [37]. However in this same range of frequency there are strong contributions of involuntary eye movements and fundus pulsations, which prevents us from measuring a dynamic signal.…”

Section: Arteriovenous Differentiation In the Retina And Choroidmentioning

confidence: 99%

“…We have previously used LDH to make full-field non-directional blood flow measurements in the retina with a few milliseconds of temporal resolution [36]. We have then shown that the depth of field of a few hundreds microns provided by LDH allows to encompass both the retinal and choroidal vessels in a single imaging plane, and LDH is able to reveal non-invasively the choroidal vasculature while providing quantitative Doppler frequency shift measurements [37]. We have used this feature to show the large differences of blood flow existing between arteries and veins in the choroid, and proposed an arteriovenous differentiation of choroidal vessels based on a flow analysis.…”

Section: Introductionmentioning

confidence: 99%

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Waveform analysis of human retinal and choroidal blood flow with laser Doppler holography

Puyo¹,

Pâques²,

Fink³

et al. 2019

Biomed. Opt. Express

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Laser Doppler holography was introduced as a full-field imaging technique to measure blood flow in the retina and choroid with an as yet unrivaled temporal resolution. We here investigate separating the different contributions to the power Doppler signal in order to isolate the flow waveforms of vessels in the posterior pole of the human eye. Distinct flow behaviors are found in retinal arteries and veins with seemingly interrelated waveforms. We demonstrate a full field mapping of the local resistivity index, and the possibility to perform unambiguous identification of retinal arteries and veins on the basis of their systolodiastolic variations. Finally we investigate the arterial flow waveforms in the retina and choroid and find synchronous and similar waveforms, although with a lower pulsatility in choroidal arteries. This work demonstrates the potential held by laser Doppler holography to study ocular hemodynamics in healthy and diseased eyes.

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“…The correction of ocular aberrations using adaptive optics (AO) and its combination with OCT and OCTA provide high-resolution images to delineate retinal pigment epithelium (RPE) from CC and assessment of CC diameter and density [ 9 , 10 , 11 ]. Recently, laser Doppler holography has been shown to provide high-resolution images of the choroidal vessels with differentiation of both arterial and venous vasculature based on the blood flow dynamics [ 12 ].…”

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confidence: 99%