High-speed micro-particle manipulation in a microfluidic chip by directional femtosecond laser impulse

Zhou, Hong; Okano, Kazunori; Carlo, Dino Di; Tanaka, Yo; Yalikun, Yaxiaer; Hosokawa, Yoichiroh

doi:10.1016/j.sna.2019.111566

Cited by 14 publications

(12 citation statements)

References 24 publications

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“…In order to resolve this issue, we switched the laser focal point from the channel center to the pocket structure on the channel wall. In the pocket, the impulsive force induced is less dispersed and can focus on the sorting area 34 .…”

Section: Resultsmentioning

confidence: 99%

“…We implement the method into a femtosecond-laser-assisted triple-selective system. A pocket structure is put on the channel wall next to the fluorescence detection region to ensure that all impulsive forces travel unidirectionally and focus at the sorting region 34 and to prevent the potential negative influence induced by the laser irradiation on the target. The pocket structure for laser irradiation is integrated into the microchannel to increase the distance (it should be > 10 μm 35 ) between the focus point and the target.…”

Section: Introductionmentioning

confidence: 99%

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Microsecond cell triple-sorting enabled by multiple pulse irradiation of femtosecond laser

Kiya

Tang

Tanaka

et al. 2023

Sci Rep

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Femtosecond-laser-assisted cell manipulation, as one of the high throughput cell sorting techniques, is tailored for single-step multiple sorting based on controllable impulsive force. In this paper, femtosecond laser pulses are focused within a pocket structure and they induce an impulse force acting on the flowing objects. The impulsive force is shown to be controllable by a new method to adjust the femtosecond pulse properties. This allows precise streamline manipulation of objects having various physical qualities (e.g., weight and volume). The pulse energy, pulse number, and pulse interval of the femtosecond laser are altered to determine the impulsive force strength. The method is validated in single cell or bead triple-sorting experiments and its capability to perform streamline manipulation in as little as 10 μs is shown. The shift profiles of the beads acting under the impulsive force are studied in order to better understand the sorting mechanism. Additionally, beads and cells with different fluorescence intensities are successfully detected and directed into different microchannels, with maximum success rates of 90% and 64.5%, respectively. To sum up, all results suggest that this method has the potential to sort arbitrary subpopulations by altering the number of femtosecond pulses and that it takes the first step toward developing a single-step multi-selective system.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microsecond cell triple-sorting enabled by multiple pulse irradiation of femtosecond laser

Kiya

Tang

Tanaka

et al. 2023

Sci Rep

Self Cite

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show abstract

“…The femtosecond laser has been a useable approach in microchannel fabrication with high resolution. 32 The drilling or ablation depth of femtosecond on commonly used microchannel materials such as PMMA, glass, or PDMS is several tens of microns [33][34][35] which is enough to drill a thin mold to make a vent hole at the channel end. The drilled holes on PDMS can be controlled to tens of microns in diameter.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of a thin PDMS film with complex liquid metal electrodes embedded and its application as skin sensors

Zhang

et al. 2022

RSC Adv.

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“…It applies an expanding microbubble to generate a jet flow that can deflect the target cell, showing a rapid switching time on the scale of 100 μs and a high cell viability. Pulsed laser 19 – 21 and resistive heating 22 , 23 are the two major approaches to generating microbubbles, but their systems are complex. In the laser-induced bubble sorting system, a high-power picosecond or nanosecond pulsed laser should be accurately focused on a specific spot in the microfluidic chip.…”

Section: Introductionmentioning

confidence: 99%

Rapid switching and durable on-chip spark-cavitation-bubble cell sorter

et al. 2022

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Precise and high-speed sorting of individual target cells from heterogeneous populations plays an imperative role in cell research. Although the conventional fluorescence-activated cell sorter (FACS) is capable of rapid and accurate cell sorting, it occupies a large volume of the instrument and inherently brings in aerosol generation as well as cross-contamination among samples. The sorting completed in a fully enclosed and disposable microfluidic chip has the potential to eliminate the above concerns. However, current microfluidic cell sorters are hindered by the high complexities of the fabrication procedure and the off-chip setup. In this paper, a spark-cavitation-bubble-based fluorescence-activated cell sorter is developed to perform fast and accurate sorting in a microfluidic chip. It features a simple structure and an easy operation. This microfluidic sorter comprises a positive electrode of platinum and a negative electrode of tungsten, which are placed on the side of the main channel. By applying a high-voltage discharge on the pair of electrodes, a single spark cavitation bubble is created to deflect the target particle into the downstream collection channel. The sorter has a short switching time of 150 μs and a long lifespan of more than 100 million workable actions. In addition, a novel control strategy is proposed to dynamically adjust the discharge time to stabilize the size of the cavitation bubble for continuous sorting. The dynamic control of continuously triggering the sorter, the optimal delay time between fluorescence detection and cell sorting, and a theoretical model to predict the ideal sorting recovery and purity are studied to improve and evaluate the sorter performance. The experiments demonstrate that the sorting rate of target particles achieves 1200 eps, the total analysis throughput is up to 10,000 eps, the particles sorted at 4000 eps exhibit a purity greater than 80% and a recovery rate greater than 90%, and the sorting effect on the viability of HeLa cells is negligible.

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High-speed micro-particle manipulation in a microfluidic chip by directional femtosecond laser impulse

Cited by 14 publications

References 24 publications

Microsecond cell triple-sorting enabled by multiple pulse irradiation of femtosecond laser

Microsecond cell triple-sorting enabled by multiple pulse irradiation of femtosecond laser

Fabrication of a thin PDMS film with complex liquid metal electrodes embedded and its application as skin sensors

Rapid switching and durable on-chip spark-cavitation-bubble cell sorter

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