High-speed label-free functional photoacoustic microscopy of mouse brain in action

Yao, Junjie; Wang, Lidai; Yang, Joon-Mo; Maslov, Konstantin; Wong, Terence T. W.; Li, Lei; Huang, Chih-Hsien; Zou, Jun; Wang, Lihong V.

doi:10.1038/nmeth.3336

Cited by 584 publications

(461 citation statements)

References 19 publications

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“…Experimental validation of the new OA methods is often performed by means of mouse brain imaging in vivo [20,21]. Figure 3 represents the results of simultaneous DR/OA/US imaging of rat's head.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous in vivo imaging of diffuse optical reflectance, optoacoustic pressure, and ultrasonic scattering

Subochev¹,

Orlova²,

Mikhailova³

et al. 2016

Biomed. Opt. Express

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Abstract:We present reflection-mode bioimaging system providing complementary optical, photoacsoutic and acoustic measurements by acoustic detector after each laser pulse. While the photons absorbed within the sample provide optoacoustic (OA) signals, the photons absorbed by the external electrode of a detector provide the measurable diffuse reflectance (DR) from the sample and the probing ultrasonic (US) pulse. To demonstrate the in vivo capabilities of the system we present the results of complementary DR/OA/US imaging of a mouse tumor, head of a newborn rat, and the back of a newborn rat with 3.5mm/50μm/35μm lateral resolution. Trimodal approach allows visualization of mechanical structures in healthy and pathological tissues along with peculiarities of blood supply. The system may be used for diagnostics of diseases accompanied by the defects of vascularization as well as for assessing the mechanisms of vascular changes when monitoring response to therapy. and optically mediated ultrasound microscopy: phantom study," Opt. Lett. 37(22), 4606-4608 (2012). 12. P. Subochev, A. Orlova, M. Shirmanova, A. Postnikova, and I. Turchin, "Simultaneous photoacoustic and optically mediated ultrasound microscopy: an in vivo study," Biomed. Opt. Express 6(2), 631-638 (2015). 13. P. Subochev, I. Fiks, M. Frenz, and Turchin, "Simultaneous triple-modality imaging of diffuse reflectance, optoacoustic pressure and ultrasonic scattering using an acoustic-resolution photoacoustic microscope: feasibility study," Laser Phys. Lett. 13(2), 025605 (2016). 14. P. Subochev, "Cost-effective imaging of optoacoustic pressure, ultrasonic scattering, and optical diffuse reflectance with improved resolution and speed," Opt. Lett. 41(5), 1006-1009 (2016).

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Section: Resultsmentioning

confidence: 99%

Simultaneous in vivo imaging of diffuse optical reflectance, optoacoustic pressure, and ultrasonic scattering

Subochev¹,

Orlova²,

Mikhailova³

et al. 2016

Biomed. Opt. Express

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“…Photoacoustic (PA) tomography (PAT) combines non-ionizing photons and low-scattering ultrasound to achieve high optical contrast and high spatial resolution imaging at depths beyond the optical diffusion limit, given by the transport mean free path (l 0 t ) [3][4][5]. Nevertheless, conventional PAT commonly treats the absorption coefficient as a scalar variable, which does not take the absorption anisotropy of biological tissue into consideration.…”

Section: Introductionmentioning

confidence: 99%

Dichroism-sensitive photoacoustic computed tomography

Qu¹,

Li²,

Shen³

et al. 2018

Optica

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Photoacoustic computed tomography (PACT), a fast-developing modality for deep tissue imaging, images the spatial distribution of optical absorption. PACT usually treats the absorption coefficient as a scalar. However, the absorption coefficients of many biological tissues exhibit an anisotropic property, known as dichroism or diattenuation, which depends on molecular conformation and structural alignment. Here, we present a novel imaging method called dichroism-sensitive PACT (DS-PACT), which measures both the amplitude of tissue's dichroism and the orientation of the optic axis of uniaxial dichroic tissue. By modulating the polarization of linearly polarized light and measuring the alternating signals through lock-in detection, DS-PACT can boost dichroic signals from biological tissues. To validate the proposed approach, we experimentally demonstrated the performance of DS-PACT by imaging plastic polarizers and ex vivo bovine tendons deep inside scattering media. We successfully detected the orientation of the optic axis of uniaxial dichroic materials, even at a depth of 4.5 transport mean free paths. We anticipate that the proposed method will extend the capability of PACT to imaging tissue absorption anisotropy.

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“…Apparatuses based on PAT have recently entered the commercial market, using optical imaging to make it much cheaper and faster than MR-based approaches. However, so far imaging depths are generally limited to a few millimeters up to a centimeter, not deep enough to reach human organs such as the heart, although some experimental studies seem promising [5,6].…”

Section: Introductionmentioning

confidence: 99%

“…Measuring the oxygenation has been demonstrated using both PAT [6] and UOT [7]. For PAT, it remains a challenge to obtain an absolute value [8], but it is possible using multiple wavelengths [9].…”

Section: Introductionmentioning

confidence: 99%

Analysis of the potential for non-invasive imaging of oxygenation at heart depth, using ultrasound optical tomography (UOT) or photo-acoustic tomography (PAT)

Walther¹,

Rippe²,

Wang³

et al. 2017

Biomed. Opt. Express

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Despite the important medical implications, it is currently an open task to find optical non-invasive techniques that can image deep organs in humans. Addressing this, photo-acoustic tomography (PAT) has received a great deal of attention in the past decade, owing to favorable properties like high contrast and high spatial resolution. However, even with optimal components PAT cannot penetrate beyond a few centimeters, which still presents an important limitation of the technique. Here, we calculate the absorption contrast levels for PAT and for ultrasound optical tomography (UOT) and compare them to their relevant noise sources as a function of imaging depth. The results indicate that a new development in optical filters, based on rare-earth-ion crystals, can push the UOT technique significantly ahead of PAT. Such filters allow the contrastto-noise ratio for UOT to be up to three orders of magnitude better than for PAT at depths of a few cm into the tissue. It also translates into a significant increase of the image depth of UOT compared to PAT, enabling deep organs to be imaged in humans in real time. Furthermore, such spectral holeburning filters are not sensitive to speckle decorrelation from the tissue and can operate at nearly any angle of incident light, allowing good light collection. We theoretically demonstrate the improved performance in the medically important case of non-invasive optical imaging of the oxygenation level of the frontal part of the human myocardial tissue. Our results indicate that further studies on UOT are of interest and that the technique may have large impact on future directions of biomedical optics.

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High-speed label-free functional photoacoustic microscopy of mouse brain in action

Cited by 584 publications

References 19 publications

Simultaneous in vivo imaging of diffuse optical reflectance, optoacoustic pressure, and ultrasonic scattering

Simultaneous in vivo imaging of diffuse optical reflectance, optoacoustic pressure, and ultrasonic scattering

Dichroism-sensitive photoacoustic computed tomography

Analysis of the potential for non-invasive imaging of oxygenation at heart depth, using ultrasound optical tomography (UOT) or photo-acoustic tomography (PAT)

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