2016
DOI: 10.1364/boe.7.003951
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Simultaneous in vivo imaging of diffuse optical reflectance, optoacoustic pressure, and ultrasonic scattering

Abstract: Abstract:We present reflection-mode bioimaging system providing complementary optical, photoacsoutic and acoustic measurements by acoustic detector after each laser pulse. While the photons absorbed within the sample provide optoacoustic (OA) signals, the photons absorbed by the external electrode of a detector provide the measurable diffuse reflectance (DR) from the sample and the probing ultrasonic (US) pulse. To demonstrate the in vivo capabilities of the system we present the results of complementary DR/OA… Show more

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Cited by 20 publications
(11 citation statements)
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“…Raster-scan optoacoustic angiography was performed using the previously developed system [ 35 , 37 ]. A lateral/axial OA resolution of 50 μm/35 μm was provided by a focal spot of 35 MHz polyvinylidene fluoride detector with a 30 MHz bandwidth, F = 6.8 mm focal distance, and 0.6 numerical aperture.…”
Section: Methodsmentioning
confidence: 99%
“…Raster-scan optoacoustic angiography was performed using the previously developed system [ 35 , 37 ]. A lateral/axial OA resolution of 50 μm/35 μm was provided by a focal spot of 35 MHz polyvinylidene fluoride detector with a 30 MHz bandwidth, F = 6.8 mm focal distance, and 0.6 numerical aperture.…”
Section: Methodsmentioning
confidence: 99%
“…Reflection-mode OA angiography of the mouse head (7.5 × 7.5 mm 2 field of view) was performed with 25 µm lateral scanning steps provided by two computer-driven stages M-664 (PI, Germany). Optically-generated probing acoustic pulses excited in the PVDF detector due to the absorption of backscattered laser radiation were utilized for complementary ultrasonic imaging of the same 7.5 × 7.5 mm 2 area [28]. To perform cerebral OA angiography at the wavelength of 1064 nm, a four-week-old nu/nu mouse and 3-day-old rat with intact skull and scalp were used (Fig.…”
Section: In Vivo Experiments and Data Processingmentioning
confidence: 99%
“…The OA imaging setup ( Figure 3) employed the concept of dark-field acoustic resolution photoacoustic microscopy (AR-PAM) [20], where the scanning OA head is represented by a conical fiber-optic illumination system (CeramOptec, Germany) combined with a spherically focused acoustic detector made of a polyvinylidene difluoride (PVDF) piezo film with a 25 μm thickness (IAP RAS, Russia) [21]. The ultrasound antenna with a numerical aperture of 0.6 and a receiving band of 35 MHz provided a spatial resolution of 40 μm at a probing depth of up to 3 mm.…”
Section: Optoacoustic (Oa) Imaging Setupmentioning
confidence: 99%