High SOX8 expression promotes tumor growth and predicts poor prognosis through GOLPH3 signaling in tongue squamous cell carcinoma

Chen, Shuwei; Li, Huan; Li, Xiyuan; Chen, Wenkuan; Xing, Zhou; Yang, Zhongyuan; Chen, Zhipeng; Chen, Jingtao; Zhang, Ying; Shi, Dingbo; Song, Ming

doi:10.1002/cam4.3041

Cited by 10 publications

(9 citation statements)

References 36 publications

(100 reference statements)

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“…21 As a member of the SOXE subfamily, SOX8 is an important functional oncogene that promotes tumor growth in tongue squamous cell carcinoma, with poor prognosis. 21,22 In the present study, SOX8 overexpression enhanced the proliferation of TNBC and decreased TNBC cell apoptosis. The expression levels of cleaved caspase-3 and cleaved caspase-9 were also evaluated, which were decreased after SOX8 overexpression.…”

Section: Discussionsupporting

confidence: 58%

<p>MicroRNA-139 Suppresses the Tumorigenicity of Triple Negative Breast Cancer Cells by Targeting SOX8</p>

Dong

Zhou

Xin

et al. 2020

CMAR

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The effects of miR-139 on the tumorigenicity of triple negative breast cancer (TNBC) and the underlying mechanisms were investigated. Methods: Normal human breast epithelial (MCF-10A) and TNBC cell lines (HCC1806 and BT549) were used for microRNA (miR)-139 overexpression, SOX8 overexpression, and knockdown studies as in vitro models of TNBC. The expression of SOX8 and miR-139 was detected by reverse transcription-polymerase chain reaction. CCK8 and clone formation assays were used to evaluate cell proliferation ability. Transwell assays and flow cytometry were used to test cell migration and apoptosis, respectively. Cell tumorigenicity was examined by tumor sphere formation assays. The interaction between miR-139 and SOX8 was examined by dual-luciferase reporter assays. The expression of SOX8, cleaved caspase-3, and cleaved caspase-9 was analyzed by Western blotting. The findings were validated in vivo using a nude mouse transplanted tumor model. Results: SOX8 expression was higher (P < 0.05) and miR-139 expression was lower (P < 0.05) in HCC1806 and BT549 cells than in MCF-10A cells. SOX8 overexpression significantly enhanced cell proliferation and migration, reduced the rate of cell apoptosis, and increased tumor sphere formation (P < 0.05) compared with the control group, whereas SOX8 knockdown had the opposite effect (P < 0.05). Overexpression of miR-139 markedly decreased cell proliferation and migration, increased cell apoptosis in vitro, and decreased tumor angiogenesis and volume in vivo (P < 0.05). Conclusion: miR-139 suppressed the tumorigenicity of TNBC cells by targeting SOX8.

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Section: Discussionsupporting

confidence: 58%

<p>MicroRNA-139 Suppresses the Tumorigenicity of Triple Negative Breast Cancer Cells by Targeting SOX8</p>

Dong

Zhou

Xin

et al. 2020

CMAR

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“…As shown on Figure 1 D, the expression of all the 50 genes identified was dramatically reduced in the LPM/ST hLECs group. Amongst them, several are known to encode proteins that regulate particularly important epithelial cell functions such as apoptosis ( ST18 , CISD3 ) [ 39 , 40 ], differentiation ( TBR1 , BARX1 , NKX1-2 , FOXB1 , APOL1 , PROP1 , TSACC ) [ 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 ], proliferation ( CEBPD , MNX1-AS1 , GPR75 , FZD9 , GAS2L3 , PFKL , TPT1-AS1 , CYP17A1 , MIR22HG ) [ 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 ], and migration ( ARHGAP9 , ITGAM , EMILIN1 , ZNF554 , SOX8 ) [ 59 , 60 , 61 , 62 , 63 ]. Moreover, the involvement of some of these genes in the cellular functions listed above was validated further by our IPA analyses.…”

Section: Resultsmentioning

confidence: 99%

Influence of the Postmortem/Storage Time of Human Corneas on the Properties of Cultured Limbal Epithelial Cells

Le‐Bel

Desjardins

Gross

et al. 2022

Cells

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Besides being a powerful model to study the mechanisms of corneal wound healing, tissue-engineered human corneas (hTECs) are sparking interest as suitable substitutes for grafting purposes. To ensure the histological and physiological integrity of hTECs, the primary cultures generated from human cornea (identified as human limbal epithelial cells (hLECs) that are used to produce them must be of the highest possible quality. The goal of the present study consisted in evaluating the impact of the postmortem/storage time (PM/ST) on their properties in culture. hLECs were isolated from the entire cornea comprising the limbus and central cornea. When grown as monolayers, short PM/ST hLECs displayed increased daily doublings and generated more colonies per seeded cells than long PM/ST hLECs. Moreover, hLECs with a short PM/ST exhibited a markedly faster wound closure kinetic both in scratch wound assays and hTECs. Collectively, these results suggest that short PM/ST hLECs have a greater number of highly proliferative stem cells, exhibit a faster and more efficient wound healing response in vitro, and produce hTECs of a higher quality, making them the best candidates to produce biomaterial substitutes for clinical studies.

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“…Dependent on m6A modification, KIAA1429 increases the stability of its downstream genes and thus elevates their expressions in cancers [15,24]. SOX8 is considered a detrimental prognostic factor for triple-negative breast cancer and tongue squamous cell cancer [19,34,35].…”

Section: Discussionmentioning

confidence: 99%

Promotive role of USP29-mediated deubiquitination in malignant proliferation of colorectal cancer cells via the KIAA1429/SOX8 axis

Jiang

Chen

et al. 2022

Bosn J of Basic Med Sci

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Colorectal cancer (CRC) is regarded as one of the most prevalent neoplasms worldwide, and ubiquitination and N6-methyladenosine (m6A) modification regulate the outgrowth of multiple cancers. This study attempted to explore the effect of ubiquitin-specific peptidases 29 (USP29) on the malignant proliferation of CRC cells via stabilizing Vir-like m6A methyltransferase associated (VIRMA/KIAA1429). First, upregulations of USP29, KIAA1429, and SRY-box transcription factor 8 (SOX8) were found in CRC tissues and cells through real-time quantitative polymerase chain reaction and Western blotting. After transfection of si-USP29, the proliferation of CRC cells was evaluated by the cell counting kit-8, colony formation and 5-ethynyl-2’-deoxyuridine assays, and we observed that depletion of USP29 inhibited the proliferation of CRC cells. Co-immunoprecipitation confirmed the binding of USP29 to KIAA1429. Mechanically, USP29 mediated deubiquitination to stabilize the protein levels of KIAA1429, and KIAA1429 promoted the stability of SOX8 mRNA through m6A modification. Moreover, overexpression of KIAA1429 or SOX8 reversed the inhibitory effects of USP29 depletion on CRC cell proliferation. Finally, the xenograft tumor model revealed the promotive role of USP29 in the proliferation of CRC cells in vivo. Altogether, USP29 facilitates the malignant proliferation of CRC cells via upregulating the KIAA1429/SOX8 axis.

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High SOX8 expression promotes tumor growth and predicts poor prognosis through GOLPH3 signaling in tongue squamous cell carcinoma

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 10 publications

References 36 publications

<p>MicroRNA-139 Suppresses the Tumorigenicity of Triple Negative Breast Cancer Cells by Targeting SOX8</p>

<p>MicroRNA-139 Suppresses the Tumorigenicity of Triple Negative Breast Cancer Cells by Targeting SOX8</p>

Influence of the Postmortem/Storage Time of Human Corneas on the Properties of Cultured Limbal Epithelial Cells

Promotive role of USP29-mediated deubiquitination in malignant proliferation of colorectal cancer cells via the KIAA1429/SOX8 axis

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