High-sensitivity enzyme thermistor determination of L-lactate by substrate recycling

Enzyme electrodes for ADP/ATP with enhanced sensitivity due to chemical amplification and intermediate accumulation

“…It is thus possible to decrease the detection limit from the 10-6-10-7 M range, typical for enzyme electrodes, to the lo-' M range. This has been demonstrated for pyruvate, which was recycled back to lactate in either an enzyme thermistor [ 2 ] or an oxygen electrode [3-51 using lactate oxidase and lactate dehydrogenase. Oxygen is consumed during oxidation of NADH to NAD' within the cycle used for the lactate determination.…”

Fabrication and Evaluation of Nanoparticle‐Based Biosensors

“…The heat capacity of organic solvents is generally 2 or 3 times lower than that of aqueous solvents [28] , ensuring better sensitivities and detection limits in the organic solvents for the same value of enthalpy change per mole. A further improvement in the sensitivity can be achieved by recycling the coenzyme or the substrate [29] .…”

Detection of Adrenaline Based on Bioelectrocatalytical System to Support Tumor Diagnostic Technology

“…Several sensors have been proposed for the detection of adrenaline or other catecholamines by using recycling processes for achieving signal amplification and to improve lower detection limit [5][6][7][8][9]. However, the optimum working pH of the described sensors does not comply with the pH value of blood that limits their application for the adrenaline detection in real blood samples.…”