Detection of Adrenaline Based on Bioelectrocatalytical System to Support Tumor Diagnostic Technology

Molinnus, Denise; Hardt, Gabriel; Käver, Larissa; Willenberg, Holger S.; Poghossian, Arshak; Keusgen, Michael; Schöning, Michael J.

doi:10.3390/proceedings1040506

Cited by 3 publications

(2 citation statements)

References 10 publications

(9 reference statements)

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“…Alvarez and Ferapontova [33] developed an RNA-based dopamine aptasensor, which has a sub-micromolar detection limit. In addition, recent reports have shown the development of different catecholamine biosensors using enzymes such as PQQ-dependent glucose dehydrogenase [34] and polyphenol oxidases [35]. Furthermore, Winiarski and his collaborators managed to reuse waste from the steel industry as a sustainable electrode modifier material for the electrochemical monitoring of different neurotransmitters [36].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Dopamine Electrochemical Detection with Manganese Doped Crystalline Copper Oxide

et al. 2023

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Manganese doped crystalline copper oxide (CuO:Mn) and undoped CuO were prepared at room temperature by the hydrothermal method. The complete physico-chemical characterization of the materials was performed using X-ray diffraction (XRD), transmission/scanning electron microscopy (TEM/SEM), and X-ray photoelectron spectroscopy (XPS). Furthermore, their analytical applicability was tested in electrochemical experiments for a dopamine assay. According to the morphological investigation, the materials had a flat structure with nearly straight edges. The XRD analysis proved the formation of the CuO phase with good crystallinity, while the Mn doping was determined by XPS to be around 1 at.%. Under optimized conditions, at pH 5.0, the CuO:Mn modified electrode (CuO:Mn/SPE) showed a high signal for dopamine oxidation, with a linear response in the 0.1–1 µM and 1–100 µM ranges and a low limit of detection of 30.3 nM. Five times higher sensitivity for manganese doped copper oxide in comparison with the undoped sample was achieved. The applicability of the developed CuO:Mn/SPE electrode was also tested in a commercially available pharmaceutical drug with good results, suggesting that the developed sensor has promising biomedical application potential.

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Section: Introductionmentioning

confidence: 99%

Enhancement of Dopamine Electrochemical Detection with Manganese Doped Crystalline Copper Oxide

et al. 2023

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“…Electrochemical biosensors offer a range of advantages over these techniques, such as selectivity, sensitivity and low limits of detection as demonstrated by Alvarez and Ferapontova [ 32 ] with their RNA-based dopamine aptasensor showing sub-micromolar detection limit. Furthermore, a variety of enzyme-based catecholamine biosensors employing monoamine oxidase [ 33 ], cellobiose dehydrogenase [ 34 ], PQQ-dependent glucose dehydrogenase [ 35 ] and polyphenol oxidases [ 36 ] have been reported recently.…”

Section: Introductionmentioning

confidence: 99%

Biosensing Dopamine and L-Epinephrine with Laccase (Trametes pubescens) Immobilized on a Gold Modified Electrode

2022

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Engineering electrode surfaces through the electrodeposition of gold may provide a range of advantages in the context of biosensor development, such as greatly enhanced surface area, improved conductivity and versatile functionalization. In this work we report on the development of an electrochemical biosensor for the laccase-catalyzed assay of two catecholamines—dopamine and L-epinephrine. Variety of electrochemical techniques—cyclic voltammetry, differential pulse voltammetry, electrochemical impedance spectroscopy and constant potential amperometry have been used in its characterization. It has been demonstrated that the laccase electrode is capable of sensing dopamine using two distinct techniques—differential pulse voltammetry and constant potential amperometry, the latter being suitable for the assay of L-epinephrine as well. The biosensor response to both catecholamines, examined by constant potential chronoamperometry over the potential range from 0.2 to −0.1 V (vs. Ag|AgCl, sat KCl) showed the highest electrode sensitivity at 0 and −0.1 V. The dependencies of the current density on either catecholamine’s concentration was found to follow the Michaelis—Menten kinetics with apparent constants KMapp = 0.116 ± 0.015 mM for dopamine and KMapp = 0.245 ± 0.031 mM for L-epinephrine and linear dynamic ranges spanning up to 0.10 mM and 0.20 mM, respectively. Calculated limits of detection for both analytes were found to be within the sub-micromolar concentration range. The biosensor applicability to the assay of dopamine concentration in a pharmaceutical product was demonstrated (with recovery rates between 99% and 106%, n = 3).

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DWINE Your Fear—Defensive Device for Women in Need

Pyngas

Chowdary

Kavitha

2019

Intelligent Systems Reference Library

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Detection of Adrenaline Based on Bioelectrocatalytical System to Support Tumor Diagnostic Technology

Cited by 3 publications

References 10 publications

Enhancement of Dopamine Electrochemical Detection with Manganese Doped Crystalline Copper Oxide

Enhancement of Dopamine Electrochemical Detection with Manganese Doped Crystalline Copper Oxide

Biosensing Dopamine and L-Epinephrine with Laccase (Trametes pubescens) Immobilized on a Gold Modified Electrode

DWINE Your Fear—Defensive Device for Women in Need

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