High sensitivity DNA detection using gold nanoparticle functionalised polyaniline nanofibres

Spain, Elaine; Kojima, Robert W.; Kaner, Richard B.; Wallace, Gordon G.; O’Grady, Justin; Lacey, Katrina; Barry, Thomas; Keyes, Tia E.; Forster, Robert J.

doi:10.1016/j.bios.2010.11.017

Cited by 68 publications

(30 citation statements)

References 42 publications

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“…To investigate the basic electrochemical properies of the CPNFs and CPANFs, the cyclic voltammetry (CV) analysis was conducted in a 1 M H 2 SO 4 electrolyte solution at different scan rates ranging from 20 mV/s to 500 mV/s. 45 Fig. 8a shows pairs of broad redox peaks with similar shapes, which correspond to the two redox processes, namely, the leucoemeraldine-emeraldine and emeraldine-pernigraniline transformations.…”

Section: Resultsmentioning

confidence: 96%

Fabrication of Gold Nanoparticles Modified Carbon Nanofibers/Polyaniline Electrode for H₂O₂Determination

Bao

Zhang

et al. 2014

J. Electrochem. Soc.

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Herein, a facile route to fabrication of carbon nanofibers/polyaniline/Au nanofibers (CPANFs) with tunable densities of gold nanoparticles (AuNPs) was proposed, and the potential application of the CPANFs as efficient electrochemical detector of H 2 O 2 was demonstrated. First, carbon nanofibers (CNFs) were successfully obtained through the carbonization procedure using electrospun polyacrylonitrile (PAN) nanofibers as a precursor. Then, polyaniline (PANI) nanorods were coated on the CNFs by an in situ polymerization of aniline, in which AuNPs were homogenously distributed via gold-thiol binding interactions. Finally, an aqueous nafion solution was used to attach HRP-modified CPANFs to the pretreated glassy carbon electrode (GCE) for H 2 O 2 detection. The fabricated electrochemical detectors for H 2 O 2 exhibited a high sensitivity for detection of H 2 O 2 with a detection limit of 0.18 μM at signal-to-noise ratio of 3.Nanoelectrode-based biosensors have been researched widely in recent years, since nanoelectrodes possess considerable advantages over conventional electrodes, such as higher spatial resolution, lower background noise, and voltammetric time scales. Due to these advantages, biosensors based on nanoelectrodes have gradually been incorporated into target detection for various fields. Hydrogen peroxide (H 2 O 2 ) is of particular interest because, as a strong oxidant and an industrial waste product, excess amounts of H 2 O 2 in rain and groundwater could negatively impact the environment. [1][2][3] As reported in the literature, sensors are often composed of two functional materials to achieve the recognition and transduction processes. Therefore, the majority of recent research on biosensors has focused on the development of modified electrode materials with high sensitivity and transduction. As a type of conducting polymer, PANI has become an attractive class of materials for a variety of advanced properties similar to metal materials, while retaining the flexibility and processability of conventional polymers. Typically, the use of individual materials has intrinsic defects, 4-6 so to improve upon electronic transduction, scientists combine PANI with carbon materials to form nanocomposites with superior performance. 7-13 CNFs are one of the most promising materials for fabrication of nanoelectrodes because of their chemical stability, high surface area, electrical conductivity, and relatively low cost.AuNPs are known to exhibit excellent optical and electronic properties in addition to their pronounced biocompatibility, which have been introduced onto the surface of conducting polymers for gene delivery 14,15 and development of biosensors. 16 Furthermore, AuNPs have been extensively used in electrochemical sensors due to their unique physicochemical properties. [17][18][19][20][21][22] With regards to small, welldispersed AuNPs grafted onto substrate materials, the fabrication of biosensors with low detection limits and a wide dynamic range for H 2 O 2 has become an intractable challenge for scienti...

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Section: Resultsmentioning

confidence: 96%

Fabrication of Gold Nanoparticles Modified Carbon Nanofibers/Polyaniline Electrode for H₂O₂Determination

Bao

Zhang

et al. 2014

J. Electrochem. Soc.

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“…The well-dispersed AuNPs solution shows red color, whereas the color will changes from red to blue or purple once AuNPs aggregate [13]. Based on the distinct color change, AuNPs have been employed as a signal transducer on varieties of platforms for many analytes, such as DNA, protein, small molecule and metal ion [14][15][16][17]. The major advantage of AuNPs based methods is that the detection event can be simply transformed into color change, which can be easily observed by the bare eye without use other complicated or expensive instruments.…”

Section: Introductionmentioning

confidence: 99%

A colorimetric method for the determination of xanthine based on the aggregation of gold nanoparticles

Zhao

et al. 2014

Microchim Acta

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We describe a highly sensitive method for the determination of traces of xanthine based on the aggregation of citrate-stabilized gold nanoparticles (AuNPs). It is found that, under optimal conditions of pH, the imide group of xanthine is adsorbed on the surface of the AuNPs, thereby displacing citrate ions. This leads to an aggregation of the AuNPs via hydrogen-bond interactions. As a result, the color of the solution changes from red to blue which can be seen with bare eyes and also can be measured by spectrophotometry. The ratio of the absorbances at 630 nm and 520 nm is linearly related to the concentration of xanthine in the 125 nM to 6.0 μM range (r=0.9988), and the detection limit (3σ/slope) is 23 nM. The method is simple, feasible and fast.

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“…On the AuNP/PAN nanotube membrane modified glassy carbon electrodes, as demonstrated by Feng [20], the synergistic effect of the two kinds of nanomaterials could enhance dramatically the sensitivity for the DNA hybridization recognition. Spain also [21] developed a DNA sensor based on the AuNP-modified PAN nanofibres. The AuNPs not only increased the area available for DNA immobilization, but also efficiently decreased the interactions between adjacent PAN chains and/or motional broadening, leading to a wide dynamic range, excellent ability to discriminate DNA mismatches, and a high sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Oxidation and sensing of ascorbic acid and dopamine on self-assembled gold nanoparticles incorporated within polyaniline film

Chu

Zhou

et al. 2015

Applied Surface Science

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High sensitivity DNA detection using gold nanoparticle functionalised polyaniline nanofibres

Cited by 68 publications

References 42 publications

Fabrication of Gold Nanoparticles Modified Carbon Nanofibers/Polyaniline Electrode for H₂O₂Determination

Fabrication of Gold Nanoparticles Modified Carbon Nanofibers/Polyaniline Electrode for H₂O₂Determination

A colorimetric method for the determination of xanthine based on the aggregation of gold nanoparticles

Oxidation and sensing of ascorbic acid and dopamine on self-assembled gold nanoparticles incorporated within polyaniline film

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High sensitivity DNA detection using gold nanoparticle functionalised polyaniline nanofibres

Cited by 68 publications

References 42 publications

Fabrication of Gold Nanoparticles Modified Carbon Nanofibers/Polyaniline Electrode for H2O2Determination

Fabrication of Gold Nanoparticles Modified Carbon Nanofibers/Polyaniline Electrode for H2O2Determination

A colorimetric method for the determination of xanthine based on the aggregation of gold nanoparticles

Oxidation and sensing of ascorbic acid and dopamine on self-assembled gold nanoparticles incorporated within polyaniline film

Contact Info

Product

Resources

About

Fabrication of Gold Nanoparticles Modified Carbon Nanofibers/Polyaniline Electrode for H₂O₂Determination

Fabrication of Gold Nanoparticles Modified Carbon Nanofibers/Polyaniline Electrode for H₂O₂Determination