High sensitivity, continuous flow thermochemical analyzer

Schifreen, Richard S.; Miller, Carolyn Sue.; Carr, Peter W.

doi:10.1021/ac50038a029

Cited by 14 publications

(3 citation statements)

References 19 publications

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“…The dependence of the lipolytic enzymes' behavior on the physicochemical properties of lipid aggregates is well documented in the literature. ,,− ,− Indeed, the activity of lipases and phospholipases may be very different for a given substrate as a function of the molecule organization. In the search of a correlation among Al III , molecule organization, and enzyme response, the enzymatic reactions were performed in lipid monolayers using CRL , and PLA 2 50, 83 from porcine pancreas in the presence and in the absence of Al. The enzymatic reactions were systematically preceded by a physicochemical characterization of monolayer properties using the Langmuir technique.…”

Section: Resultsmentioning

confidence: 99%

Impact of Aluminum on the Oxidation of Lipids and Enzymatic Lipolysis in Monomolecular Films at the Air/Water Interface

et al. 2007

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There is evidence that serious pathologies are associated with aluminum (Al). In the present work, the influence of Al on enzymatic lipolysis was studied with the aim to get more insight into the possible link between the Al-induced membrane modification and the cytotoxicity of the trivalent cation (AlIII). Lipid monolayers were used as model membranes. The monomolecular film technique allowed monitoring the Al-dependent modifications of the lipid monolayer properties and enzyme kinetics. Two enzymes, namely, Candida rugosa lipase and a calcium (CaII)-dependent phospholipase A2 from porcine pancreas, were used to catalyze the lipolysis of triglyceride and phosphoglyceride monolayers, respectively. The results obtained show that Al modifies both the monolayer structure and enzymatic reaction rates. While the enzymes used in this study can be considered as probes detecting lipid membrane properties, it cannot be excluded that in physiological conditions modulation of the enzyme action by the Al-bound membranes is among the reasons for Al toxicity.

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Section: Resultsmentioning

confidence: 99%

Impact of Aluminum on the Oxidation of Lipids and Enzymatic Lipolysis in Monomolecular Films at the Air/Water Interface

et al. 2007

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“…We feel that in other areas also, thermal flow stream analysis will be put to use increasingly in the future. One is the application for pure thermochemical analysis (25). For routine biochemical analysis, enzyme thermistors or corresponding probes incorporating biological sensors will probably find application.…”

Section: Discussionmentioning

confidence: 99%

Thermal bioanalyzers in flow streams. Enzyme thermistor devices

Mosbach¹,

Danielsson²

1981

Anal. Chem.

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“…This can be achieved by means of a differential measuring system in which two thermistors are used in a Wheatstone bridge arrangement. One thermistor is situated at the end of the adiabatic reaction coil and the reference thermistor is placed in the same stream after passing a stainless steel heat exchanging equilibration coil [2].…”

Section: Introductionmentioning

confidence: 99%