Impact of Aluminum on the Oxidation of Lipids and Enzymatic Lipolysis in Monomolecular Films at the Air/Water Interface

Abstract

There is evidence that serious pathologies are associated with aluminum (Al). In the present work, the influence of Al on enzymatic lipolysis was studied with the aim to get more insight into the possible link between the Al-induced membrane modification and the cytotoxicity of the trivalent cation (AlIII). Lipid monolayers were used as model membranes. The monomolecular film technique allowed monitoring the Al-dependent modifications of the lipid monolayer properties and enzyme kinetics. Two enzymes, namely, Candida rugosa lipase and a calcium (CaII)-dependent phospholipase A2 from porcine pancreas, were used to catalyze the lipolysis of triglyceride and phosphoglyceride monolayers, respectively. The results obtained show that Al modifies both the monolayer structure and enzymatic reaction rates. While the enzymes used in this study can be considered as probes detecting lipid membrane properties, it cannot be excluded that in physiological conditions modulation of the enzyme action by the Al-bound membranes is among the reasons for Al toxicity.

Hydrolysis of mixed monomolecular films of tricaprylin/dilauroylphosphatidylcholine by lipase and phospholipase A2

“…The DiC12PC exhibits an isotherm characteristic of a liquid expanded state with a collapse pressure about 46 mN m −1 . The curve is in agreements with prior studies [20,21]. From corresponding V(a) isotherms and Helmholtz equation ( V = 4 ⊥ ), the vertical components of dipole moment ⊥ per molecule are estimated.…”

Synergistic barrier effect of phosphatidylcholine and phosphatidic acid on the ion transfer across a polarized liquid–liquid interface and its electrochemical stability

“…P-1138), dipalmitoyl L-a-phosphatidic acid, DPPA, (Sigma, P-4393), distearoyl L-a-phosphatidylethanolamine sodium salt, DSPE, (Sigma, P-1348) and dipalmytoyl L-a-phosphatidylserine sodium salt, DPPS, (Sigma, P-1185). Solutions of La 3+ and Yb 3+ were prepared from La 2 (SO 4 ) 3 The tetrapentylammonium salt of tetrakis[3,5-bis(trifluoromethyl)phenyl]borate, TPenA + TFPB À which was added to the organic phase as a supporting electrolyte was prepared following the procedure described previously [18].…”

“…The interaction between phospholipids and multivalent metal ions such as Al 3+ and lanthanide ion causes toxic or pharmacological actions in biological organisms [1][2][3]. Such actions are considered to be involved in the modulation and stabilization of the membrane structure through the binding of metal ion with phospholipids.…”

Clay body wrap with microcurrent: Effects in central adiposity

“…In fact, for Laudánska et al (2002) human skin appears to be permeable to Ca 2+ and Mg 2+ ions, suggesting the possibility of skin penetration by these two components. Moreover, the aluminum present in clay exerts its action only in the presence of certain lipolysis enzymes, modifying them and promoting the enzyme-substrate recognition (Corvis et al, 2007;Rumberger et al, 2004).…”