High‐sensitivity capillary and microchip electrophoresis using electrokinetic supercharging

Dawod, Mohamed; Chung, Doo Soo

doi:10.1002/jssc.201100384

Cited by 31 publications

(24 citation statements)

References 71 publications

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“…Introduced nine years ago, EKS combines the efficient stacking power of FESI and the highly selective nature of tITP . Consequently, the technique provides enhancement in detection sensitivity of much higher order than solely using FASI or tITP.…”

Section: Sequential Stacking Methodsmentioning

confidence: 99%

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2010–2012)

et al. 2013

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CE has been alive for over two decades now, yet its sensitivity is still regarded as being inferior to that of more traditional methods of separation such as HPLC. As such, it is unsurprising that overcoming this issue still generates much scientific interest. This review continues to update this series of reviews, first published in Electrophoresis in 2007, with updates published in 2009 and 2011 and covers material published through to June 2012. It includes developments in the field of stacking, covering all methods from field amplified sample stacking and large volume sample stacking, through to isotachophoresis, dynamic pH junction and sweeping. Attention is also given to online or inline extraction methods that have been used for electrophoresis.

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Section: Sequential Stacking Methodsmentioning

confidence: 99%

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2010–2012)

et al. 2013

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“…20 While the separation typically takes place in a narrow-bore silica capillary in CE, it is usually performed in a planar microfluidic device in MCE. CE and MCE occupy a distinctive position within the separation science field and are considered in many ways as a hybrid between slab gel electrophoresis and LC and are usually compared with these two.…”

Section: Separation Modes and Conditionsmentioning

confidence: 99%

“…The development of efficient preconcentration strategies is of absolute necessity especially when dealing with biological samples where target proteins are usually present in low concentration. 20 …”

Section: Separation Modes and Conditionsmentioning

confidence: 99%

Recent advances in protein analysis by capillary and microchip electrophoresis

Dawod

Arvin

Kennedy

2017

Analyst

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This review article describes the significant recent advances in analysis of proteins by capillary and microchip electrophoresis during the period mid 2014 to early 2017. The review highlights the progressions, new methodologies, innovative instrumental modifications, and challenges for efficient protein analysis in human specimens, animal tissues, and plant samples. Protein analysis fields covered in this review include analysis of native, reduced, and denatured proteins in addition to Western blotting, protein therapeutics and proteomics.

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“…Subsequent works on EKS for CE and MCE have been reviewed with a major focus on theoretical and computer simulation analysis of critical factors that affect the performance of EKI and tITP . As was also highlighted in the recent review , research efforts of various groups have proven potentially universal applicability of EKS, including metal ions, pharmaceuticals, peptides, DNA fragments, and proteins, importantly both in capillary and microchip formats.…”

Section: Introductionmentioning

confidence: 99%

Electrokinetic supercharging preconcentration prior to CGE analysis of DNA: Sensitivity depends on buffer viscosity and electrode configuration

Mori

Yamada

et al. 2013

Electrophoresis

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Aiming to high sensitivity DNA analysis by CGE, electrokinetic supercharging (EKS) approach was adopted in this article. EKS is known as an online preconcentration technique that combines electrokinetic sample injection (EKI) with transient ITP (tITP). Herein, two factors of buffer viscosity and electrode configuration were studied to further improve EKS performance. An ultralow-viscosity Tris-Boric acid-EDTA (TBE) buffer solution, consisted of 2% low-molecular-weight hydroxypropyl methyl cellulose (HPMC) and 6% mannitol and with pH 8.0 adjusted by boric acid, was applied. The boric acid would make a complex with mannitol and generates borate polyanion, which acts as the leading ion for tITP process. The new electrode configuration, a Pt ring around capillary, was modified on Agilent CE system to lead large amount sample introduction during EKS. The standard DNA sample of φX174/HaeIII digest was used to evaluate the qualitative and quantitative abilities of the proposed strategy. The 170,000-fold highly diluted sample at concentration of 3.0 ng/mL was enriched by EKS and detected by normal UV detection method. The obtained LOD of the weakest peak of 72 bp fragment was around 7.7 pg/mL, apparently improved more than 10,000-fold in comparison with conventional CGE with UV detection.

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High‐sensitivity capillary and microchip electrophoresis using electrokinetic supercharging

Cited by 31 publications

References 71 publications

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2010–2012)

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2010–2012)

Recent advances in protein analysis by capillary and microchip electrophoresis

Electrokinetic supercharging preconcentration prior to CGE analysis of DNA: Sensitivity depends on buffer viscosity and electrode configuration

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