High-sensitive Biomarkers of Blood Antiradical Activity in Mice Exposed to γ-irradiation

Abstract

Diagnostic markers are important indicators for the assessment of the possible consequences of radiation exposure, which could lead to acute and chronic radiation toxicity. The goal of the study is to determine high-sensitive markers of blood redox status in relation to the dose of radiation. The mice were exposed to γ-irradiation (Cs137) at a total dose of 3, 5, and 7Gy. After 1, 2, 7, and 14 days of the irradiation, the blood samples were drawn under ether anesthesia from the inferior vena cava and the activity of antioxidant system (enzymatic -superoxide dismutase (SOD), catalase) and non-enzymatic total antioxidant activity (TAA) of blood serum was determined. The results of our investigation support the fact that the non-enzymatic antioxidant TAA plays an important role in the prevention of radiation damage during ionizing radiation exposure, which makes it possible to consider TAA as a promising candidate as the biomarker of radiation dose.

Protective Effect of Herniarin on the Ionizing Radiation-Induced Alterations of Erythrocyte Membrane Proteins

“…19 Therefore, irradiation of RBCs by gamma radiation causes the formation of reactive oxygen and nitrogen radicals, which induce the intracellular oxidative stress and rapid Tyr-phosphorylation of B3p (that revealed a decrease of the erythrocytes membrane absorption spectra at 280 nm) with dose-dependent damage of RBCs membranes. 20,21 We can assume that Herniarin reduces ROS levels in the body and prevents tyrosine phosphorylation in B3p due to its antioxidant activity. It also causes an increase in the number of erythrocytes in peripheral blood and, therefore, in the intensity of absorption spectra at 280nm in the late post-irradiation period.…”