High-risk human papillomavirus, tumor suppressor protein p53 and mitomycin-C in invasive squamous cell carcinoma cervix

Abstract

BACKGROUND: Clinical data relating to human papillomavirus (HPV) infection and p53 status in cervical cancer has been sparse and confusing. AIM: To evaluate high-risk HPV and expression of tumor suppressor protein p53 in squamous cell carcinoma of cervix and to assess response to mitomycin-C in neo-adjuvant chemotherapy. SETTING AND DESIGN: RESULTS:All patients with cancer cervix were positive for high-risk HPV DNA having relative light units/cut off values ranging from 3.4-2389.21 (P value = 0.006). High viral load of high risk HPV DNA was seen in advanced stages (P= 0.05) and an association of viral load with tumor volume was also seen (r=0.361, P=0.05). Analysis of p53 protein in cervical carcinoma patient showed expression in 50% of cancer specimens (P value < 0.001).McNemar's and Fischer's exact test showed no change in p53 status post-chemotherapy; however 66% of stage II B patients in VBP-M group became operable. CONCLUSION: High-risk HPV was universally present in all cases of cancer cervix and viral load was associated with stage and tumor volume while p53 protein was expressed in 50% of cases suggesting deregulation. More studies using mitomycin-C in cervical cancer treatment protocols are needed.

p53 and bcl2 expression in malignant and premalignant lesions of uterine cervix and their correlation with human papilloma virus 16 and 18

“…A similar staining pattern of HPV 16/18 was observed by Menon et al [26] and Choudhury and Singh. [25] In the present study, no significant statistical association could be found between HPV infection and p53 expression (P = 0.377 for CIN and 1.00 for carcinoma cervix) which was in accordance with Jeffers et al [9] and Rajaram et al [12] On the contrary, Dellas et al [10] found a significant correlation between HPV infection and p53 immunoreactivity. We also did not find any significant statistical correlation between HPV infection and bcl2 expression.…”

“…The results were in accordance with those found by several other authors. [12][13][14] However, many authors have found lower positivity rates ranging between 10% and 30% respectively. [15][16][17] In contrast, Grace et al [7] and few other workers [11,18,19] found much higher rates of p53 expression ranging between 69% and 100% in squamous cell carcinomas of the cervix.…”

“…Other authors using polymerase chain reaction, restriction fragment length polymorphism or Hybrid capture II found higher positivity rates varying between 70% and 95% for HPV 16 and HPV 18 infection in cases of CIN and carcinoma cervix. [5,7,12] The higher rates have been reported because of the use of more sensitive methods of HPV detection. In our study, a significantly higher number of carcinoma cases showed an intranuclear dot like staining pattern (P = 0.003).…”

A comparative study of p53 expression in premalignant and malignant cervical lesions at a tertiary care institute of Rohilkhand region, India

“…SCCs are the most common histological type as the most common etiological agent responsible for cervical cancer is HPV which most commonly affects the lining cervical [19,23] epithelium. HSIL was the common histological subtype in premalignant lesions in the present study which is in concordance to the studies done by Jeffers et al [24] The mean age of presentation of malignant lesions (49.04 ± 10.42 years) is almost similar to that reported by Rajaram et al [25] (52.1 ± 12.46 years), Tan et al [26] (51.1 years), Koncar et al [27] (50 years), and Tan et al [28] (50.3 years). Furthermore, the mean age of presentation of premalignant lesions (41.00 ± 8.89 years) is younger than that of malignant lesions.…”

Qualitative-based Multiplex PCR: Diagnosis of Papilloma virus Types 16 & 18 in Samples Taken from Patients with Malignant Lesions

“…Using the conserve L gene of virus capsid (20). Since only high risk types of HPV cause cervical cancer, it is necessary to determine the HPV types in clinical samples (21). Considering the extent of HPV infection, it seems necessary to determine the most prevalent HPV types in each population in order to design an effective screening program, management, and vaccination against the prevalent virus types in the population.…”