High-risk human papillomavirus infections in colorectal cancer in the Syrian population and their association with Fascin, Id-1 and P-cadherin expressions: A tissue microarray study

Epstein–Barr virus and its association with Fascin expression in colorectal cancers in the Syrian population: A tissue microarray study

“…Due to the increasing interest in exploring the possible role of viral infection in human cancers, including CRC, we have recently reported that approximately 54% of human CRC samples are positive for high-risk HPVs in the Syrian population. 26 In addition, our study revealed that the presence of high-risk HPVs is correlated with an invasive and metastatic CRC phenotype. More importantly, we were able to show that E6/E7 onco-proteins of high-risk HPV type 16 convert non-invasive and non-metastatic human cancer cells into invasive and metastatic ones.…”

“…IHC analysis investigating the expression of LMP1 and Fascin were performed using standard procedures as described previously by our group. 10,26 Primary LMP1 and Fascin antibodies were obtained from Dako (clone 1-4 and 55K-2; Dako, Canada). The sections were analyzed by Leica DMLB microscopy equipped with a Leica DFC-480 Camera (Leica Microsystems, Germany).…”

Human papillomaviruses-related cancers

“…Data of this investigation showed, for the first time, that highrisk HPVs are present in human colorectal cancers in the Syrian population and their presence is associated with invasive and metastatic phenotype. 134 On the other hand, the same group has been investigating the role of E6/E7 onco-proteins of high-risk HPVs in human normal colorectal cells; data of this study showed that E6/E7 of HPV type 16 are able to induce cellular transformation and migration of human normal colorectal mesenchymal cells but not epithelial ones; this was accompanied by upregulations of D-type cyclins and Cyclin E as well as Id-1 in these cells. 135 3) Finally: Three studies explored the presence of HPVs in colorectal cancers in Turkey using PCR analysis; the first one found that high-risk HPVs are present in 46 of 56 colorectal cancer tissues which represent 82.14% of the cases.…”

High-Risk HPVs and Human Carcinomas in the Syrian Population

“…Furthermore, the expression of E6 onco-protein of high-risk HPVs was found to be associated with Fascin, Id-1, and P-cadherin expression in the majority of cancer tissue samples. Data of this investigation showed, for the first time, that high-risk HPVs are present in human colorectal cancers in the Syrian population and their presence is associated with invasive and metastatic phenotype (69). On the other hand, it is important to indicate that the presence of HPVs in colorectal cancers in the ME is limited only to four studies one from Israel (65) and three from Turkey (70–72).…”

“…In this context, it is important to highlight that there is a few investigations about the presence of HPVs in colorectal cancers in the developing countries including one study from Syria, which was performed by the Canadian/Syrian group (Table 1). This group has investigated the presence of high-risk HPVs and their association with Fascin, Id-1, and P-cadherin genes, which are major regulators of cell invasion and metastasis (66–68), in human colorectal cancers in the Syrian population (69). In this study, the authors used PCR and tissue microarray analysis to explore the presence of HPV and E6 expression, respectively, in a cohort of 78 cancer tissues (41 females and 37 males with a median age of 49 years).…”