High-resolution visualization of tumours in rabbit lung using refraction contrast X-ray imaging

Sera, Toshihiro; Uesugi, Kentaro; Yagi, Naoto; Umetani, Keiji; Kobatake, Makito; Imai, Shigeki

doi:10.1016/j.ejrad.2008.04.030

Cited by 10 publications

(10 citation statements)

References 9 publications

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“…Gao et al studied human liver cancer and was able to visualize the guinea pig’s cochlea [23]. Lungs and lung tumors have also been widely studied [24,25]. The structure of trabecular bone [26] and the bone–cartilage interface [27] can also be distinguished by XPCI techniques.…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional reconstruction of blood vessels in the rabbit eye by X-ray phase contrast imaging

et al. 2013

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BackgroundA clear understanding of the blood vessels in the eye is helpful in the diagnosis and treatment of ophthalmic diseases, such as glaucoma. Conventional techniques such as micro-CT imaging and histology are not sufficiently accurate to identify the vessels in the eye, because their diameter is just a few microns. The newly developed medical imaging technology, X-ray phase-contrast imaging (XPCI), is able to distinguish the structure of the vessels in the eye. In this study, XPCI was used to identify the internal structure of the blood vessels in the eye.MethodsAfter injection with barium sulfate via the ear border artery, an anesthetized rabbit was killed and its eye was fixed in vitro in 10% formalin solution. We acquired images using XPCI at the Shanghai Synchrotron Radiation Facility. The datasets were converted into slices by filtered back-projection (FBP). An angiographic score was obtained as a parameter to quantify the density of the blood vessels. A three-dimensional (3D) model of the blood vessels was then established using Amira 5.2 software.ResultsWith XPCI, blood vessels in the rabbit eye as small as 18 μm in diameter and a sixth of the long posterior ciliary artery could be clearly distinguished. In the 3D model, we obtained the level 4 branch structure of vessels in the fundus. The diameters of the arteria centralis retinae and its branches are about 200 μm, 110 μm, 95 μm, 80 μm and 40 μm. The diameters of the circulus arteriosus iridis major and its branches are about 210 μm, 70 μm and 30 μm. Analysis of vessel density using the angiographic score showed that the blood vessels had maximum density in the fundus and minimum density in the area anterior to the equator (scores 0.27 ± 0.029 and 0.16 ± 0.032, respectively). We performed quantitative angiographic analysis of the blood vessels to further investigate the density of the vessels.ConclusionsXPCI provided a feasible means to determine the structure of the blood vessels in the eye. We were able to determine the diameters and morphological characteristics of the vessels from both 2D images and the 3D model. By analyzing the images, we obtained measurements of the density distribution of the microvasculature, and this approach may provide valuable reference information prior to glaucoma filtration surgery.

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Section: Introductionmentioning

confidence: 99%

Three-dimensional reconstruction of blood vessels in the rabbit eye by X-ray phase contrast imaging

et al. 2013

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“…Before imaging, the mouse was humanely sacrificed by intraperitoneal (ip) sodium pentobarbital injection overdose. The mouse was imaged after four hours of its death in order to avoid the small shifts of the body caused by the development of rigor mortis [7]. And then a piece of Kapton (Dupont, DE, USA) was used and rolled up into a tube.…”

Section: Samplesmentioning

confidence: 99%

“…For example, lung cancer is one of the most common diseases with low survival rates worldwide; early detection of the lung cancer is known to improve the chances of successful treatment. But in most cases, cancer has been detected in the terminal stage and is impossible to be cured [1,5,7]. As a result, it is of great importance to image micro-structures in lung to enable early detection.…”

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, for the large number of alveoli, the fine structures were averaged out. Other researchers like Parsons and Sera et al have utilized IL-XPCI to study lungs, and successfully generated the three-dimensional models of mouse airways [7,17]. Despite advances in lung imaging using IL-XPCI, threedimensional visualization of the alveoli in the intact animals has remained elusive.…”

Section: Introductionmentioning

confidence: 99%

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Non-Invasive Microstructure and Morphology Investigation of the Mouse Lung: Qualitative Description and Quantitative Measurement

Zhang

Luo

2011

PLoS ONE

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BackgroundEarly detection of lung cancer is known to improve the chances of successful treatment. However, lungs are soft tissues with complex three-dimensional configuration. Conventional X-ray imaging is based purely on absorption resulting in very low contrast when imaging soft tissues without contrast agents. It is difficult to obtain adequate information of lung lesions from conventional X-ray imaging.MethodsIn this study, a recently emerged imaging technique, in-line X-ray phase contrast imaging (IL-XPCI) was used. This powerful technique enabled high-resolution investigations of soft tissues without contrast agents. We applied IL-XPCI to observe the lungs in an intact mouse for the purpose of defining quantitatively the micro-structures in lung.FindingsThe three-dimensional model of the lung was successfully established, which provided an excellent view of lung airways. We highlighted the use of IL-XPCI in the visualization and assessment of alveoli which had rarely been studied in three dimensions (3D). The precise view of individual alveolus was achieved. The morphological parameters, such as diameter and alveolar surface area were measured. These parameters were of great importance in the diagnosis of diseases related to alveolus and alveolar scar.ConclusionOur results indicated that IL-XPCI had the ability to represent complex anatomical structures in lung. This offered a new perspective on the diagnosis of respiratory disease and may guide future work in the study of respiratory mechanism on the alveoli level.

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“…Tumors with size of millimeters in the rabbit lung were clearly visualized in tomography; the structure characters of lung cancer in vivo in different days after the implantation in mice were tested using phase contrast X-ray imaging, and three-dimensional image of lung tumor angiogenesis was obtained using BaSO 4 as contrast agent by SR-CT [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Imaging characters of the lung cancer phantoms under the simulative clinical condition performed with hard X-ray in-line holography

Zhang

Chen

et al. 2013

J. Inst.

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The simulative lung cancer tissues under the approximate clinical condition were imaged using in-line holography method with 35 keV synchrotron radiation hard X-ray. The millimeter scale simulative cancer phantoms showed adequate contrast to lung tissues in our experiment. It demonstrates that in-line holography method with synchrotron radiation hard X-ray promises to be a potential sensitive method for the early detection of lung cancer. The image contrast, standard deviation (SD) and normalized standard deviation (NSD) of different areas were calculated. It shows that the traditional method of contrast calculation does not always give a convincible result in image judgment; a standard deviation map of image taken with a proper distance of sample to detector (DSD) will correspond well to the projecting image and supply effective assistance in diagnostic judgment.

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High-resolution visualization of tumours in rabbit lung using refraction contrast X-ray imaging

Cited by 10 publications

References 9 publications

Three-dimensional reconstruction of blood vessels in the rabbit eye by X-ray phase contrast imaging

Three-dimensional reconstruction of blood vessels in the rabbit eye by X-ray phase contrast imaging

Non-Invasive Microstructure and Morphology Investigation of the Mouse Lung: Qualitative Description and Quantitative Measurement

Imaging characters of the lung cancer phantoms under the simulative clinical condition performed with hard X-ray in-line holography

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