High-resolution screening for marine prokaryotic and eukaryotic taxa with selective preference for PE and PET surfaces

Marsay, Katherine S.; Koucherov, Yuri; Davidov, Keren; Kounio, Evgenia Iankelevich; Itzahri, Sheli; Salmon‐Divon, Mali; Oren, Matan

doi:10.1101/2021.12.26.474179

Cited by 2 publications

(1 citation statement)

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“…Within Saprospiraceae, Portibacter was more abundant in PLA biofilms than in those on PHBV. Even if poorly characterized, this taxon has been previously reported to colonize polyethylene, polyethylene terephthalate and PLA in the marine plastisphere [ 56 , 57 , 58 ].…”

Section: Resultsmentioning

confidence: 99%

Microbiological Characterization of the Biofilms Colonizing Bioplastics in Natural Marine Conditions: A Comparison between PHBV and PLA

et al. 2023

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Biodegradable polymers offer a potential solution to marine pollution caused by plastic waste. The marine biofilms that formed on the surfaces of poly(lactide acid) (PLA) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) were studied. Bioplastics were exposed for 6 months to marine conditions in the Mediterranean Sea, and the biofilms that formed on their surfaces were assessed. The presence of specific PLA and PHBV degraders was also studied. PHBV showed extensive areas with microbial accumulations and this led to higher microbial surface densities than PLA (4.75 vs. 5.16 log CFU/cm2). Both polymers’ surfaces showed a wide variety of microbial structures, including bacteria, fungi, unicellular algae and choanoflagellates. A high bacterial diversity was observed, with differences between the two polymers, particularly at the phylum level, with over 70% of bacteria affiliated to three phyla. Differences in metagenome functions were also detected, revealing a higher presence of proteins involved in PHBV biodegradation in PHBV biofilms. Four bacterial isolates belonging to the Proteobacteria class were identified as PHBV degraders, demonstrating the presence of species involved in the biodegradation of this polymer in seawater. No PLA degraders were detected, confirming its low biodegradability in marine environments. This was a pilot study to establish a baseline for further studies aimed at comprehending the marine biodegradation of biopolymers.

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Section: Resultsmentioning

confidence: 99%

Microbiological Characterization of the Biofilms Colonizing Bioplastics in Natural Marine Conditions: A Comparison between PHBV and PLA

et al. 2023

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Differential colonization and succession dynamics of marine bacteria on different plastic polymers

Davidov,

Itzahri,

Sinberger

et al. 2024

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During the past decades since plastic was introduced to the world, marine microorganisms have been adapted for life on marine plastic debris, forming unique plastic-attached microbial communities. To date, little is known about the colonization and succession processes that take place on plastic surfaces in marine environments and how the plastic polymer type affects the plastic-attached microbiome composition. To address this knowledge gap, we examined the colonization and succession dynamics of marine bacteria on four common plastic polymers - PE, PP, PS, and PET-compared to glass and wood in a controlled seawater system under different temperatures. Using a simple experimental design, coupled with a long-read 16S rRNA metabarcoding pipeline and a set of complementary data analyses, we characterized the temporal trends in the composition of the bacterial microbiome developed on different surfaces over 2 - 90 days. By applying weighted gene co-expression network (WGCNA) analysis, we established co-occurrence networks and identified genera with specific succession signatures, significant enrichment on specific plastic polymers and/or strong intra-genus connections. Among them, members of genusAlcanivoraxwere significantly enriched on either PE or PP plastic surfaces as early as 2 days post-inoculation.Alcanivoraxcolonization preference to polyolefins was confirmed in colonization assays with pureAlcanivoraxstrains. Our research approach presented here may contribute to understanding how plastisphere communities are being formed and help identify taxa with specific adaptations to plastic surfaces.

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High-resolution screening for marine prokaryotic and eukaryotic taxa with selective preference for PE and PET surfaces

Cited by 2 publications

References 83 publications

Microbiological Characterization of the Biofilms Colonizing Bioplastics in Natural Marine Conditions: A Comparison between PHBV and PLA

Microbiological Characterization of the Biofilms Colonizing Bioplastics in Natural Marine Conditions: A Comparison between PHBV and PLA

Differential colonization and succession dynamics of marine bacteria on different plastic polymers

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