High-resolution quantification of root dynamics in split-nutrient rhizoslides reveals rapid and strong proliferation of maize roots in response to local high nitrogen

Zandt, Dina in ‘t; Marié, Chantal Le; Kirchgeßner, Norbert; Visser, Eric J. W.; Hund, Andreas

doi:10.1093/jxb/erv307

Cited by 22 publications

(27 citation statements)

References 49 publications

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“…the allele that increased the number of seminal roots (namely, B73) also decreased primary root size]. In line with our results, in ‘t Zandt et al (2015) observed a 2-fold increase in seminal root dry weight upon removal of primary root. The most likely explanation of these findings is the presence of a relocation mechanism for seed endosperm resources that apparently sustains root development irrespective of the genetic constraints on seminal roots.…”

Section: Discussionsupporting

confidence: 89%

Two major quantitative trait loci controlling the number of seminal roots in maize co-map with the root developmental genesrtcsandrum1

Salvi

Giuliani

Ricciolini

et al. 2016

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Section: Discussionsupporting

confidence: 89%

Two major quantitative trait loci controlling the number of seminal roots in maize co-map with the root developmental genesrtcsandrum1

Salvi

Giuliani

Ricciolini

et al. 2016

EXBOTJ

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“…However, in ‘t Zandt et al [29] found no direct dependence of the dynamic of roots exposed to differential N supply in the rhizoslides concluding that the responses on either side occurred largely independent from each other.…”

Section: Resultsmentioning

confidence: 99%

RADIX: rhizoslide platform allowing high throughput digital image analysis of root system expansion

et al. 2016

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BackgroundPhenotyping of genotype-by-environment interactions in the root-zone is of major importance for crop improvement as the spatial distribution of a plant’s root system is crucial for a plant to access water and nutrient resources of the soil. However, so far it is unclear to what extent genetic variations in root system responses to spatially varying soil resources can be utilized for breeding applications. Among others, one limiting factor is the absence of phenotyping platforms allowing the analysis of such interactions.ResultsWe developed a system that is able to (a) monitor root and shoot growth synchronously, (b) investigate their dynamic responses and (c) analyse the effect of heterogeneous N distribution to parts of the root system in a split-nutrient setup with a throughput (200 individual maize plants at once) sufficient for mapping of quantitative trait loci or for screens of multiple environmental factors. In a test trial, 24 maize genotypes were grown under split nitrogen conditions and the response of shoot and root growth was investigated. An almost double elongation rate of crown and lateral roots was observed under high N for all genotypes. The intensity of genotype-specific responses varied strongly. For example, elongation of crown roots differed almost two times between the fastest and slowest growing genotype. A stronger selective root placement in the high-N compartment was related to an increased shoot development indicating that early vigour might be related to a more intense foraging behaviour.ConclusionTo our knowledge, RADIX is the only system currently existing which allows studying the differential response of crown roots to split-nutrient application to quantify foraging behaviour in genome mapping or selection experiments. In doing so, changes in root and shoot development and the connection to plant performance can be investigated.Electronic supplementary materialThe online version of this article (doi:10.1186/s13007-016-0140-8) contains supplementary material, which is available to authorized users.

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“…However, in 7- or 14-day-old rice seedling, local nitrate supply only affected elongation of LRs on primary and seminal roots, and LR number was not changed ( Wang et al , 2002 ). In 7-day-old maize seedlings, only elongation of LRs on primary roots is induced ( P. Yu et al , 2014 ), but older maize seedlings show alterations in the density and length of LRs on crown roots by local nitrate treatment ( in ‘t Zandt et al , 2015 ). Thus, the LRs of various cereal species respond differently to nitrate, implying that both species-specific and developmental stage-specific effects are involved.…”

Section: Lateral Root and Root Hair Development Response To External mentioning

confidence: 99%

Nitrate regulation of lateral root and root hair development in plants

Liu

Yang

et al. 2019

Journal of Experimental Botany

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Nitrogen (N) is one of the most important macronutrients for plant growth and development. However, the concentration and distribution of N varies in soil due to a variety of environmental factors. In response, higher plants have evolved a developmentally flexible root system to efficiently take up N under N-limited conditions. Over the past decade, significant progress has been made in understanding this form of plant ‘root-foraging’ behavior, which is controlled by both a local and a long-distance systemic nitrate signaling pathway. In this review, we focus on the key components of nitrate perception, signaling, and transduction and its role in lateral root development. We also highlight recent findings on the molecular mechanisms of the nitrate systemic signaling pathway, including small signaling peptides involved in long-distance shoot–root communication. Furthermore, we summarize the transcription factor networks responsible for nitrate-dependent lateral root and root hair development.

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High-resolution quantification of root dynamics in split-nutrient rhizoslides reveals rapid and strong proliferation of maize roots in response to local high nitrogen

Abstract: HighlightPatches rich in nitrogen are rapidly colonized by selective root growth in maize, which was quantified at high time resolution with state-of-the-art non-invasive imaging techniques in a paper-based growth system.

Cited by 22 publications

References 49 publications

Two major quantitative trait loci controlling the number of seminal roots in maize co-map with the root developmental genesrtcsandrum1

Two major quantitative trait loci controlling the number of seminal roots in maize co-map with the root developmental genesrtcsandrum1

RADIX: rhizoslide platform allowing high throughput digital image analysis of root system expansion

Nitrate regulation of lateral root and root hair development in plants

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