High-Resolution Patterned Cellular Constructs by Droplet-Based 3D Printing

Graham, Alexander; Olof, Sam N.; Burke, Madeline; Armstrong, James R.; Mikhailova, Ellina; Nicholson, James; Box, Stuart J.; Szele, Francis G.; Perriman, Adam W.; Bayley, Hagan

doi:10.1038/s41598-017-06358-x

Cited by 171 publications

(179 citation statements)

References 65 publications

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“…[ 18 ] Using a droplet‐based bioprinting, Graham et al generated a renal‐like tissue using human embryonic kidney cells (HEK cells) and mesenchymal stem cells. [ 19 ]…”

Section: Evolution Of In Vitro Methods For Drug Screeningmentioning

confidence: 99%

Grafting of 3D Bioprinting to In Vitro Drug Screening: A Review

Nie

Gao

et al. 2020

Adv Healthcare Materials

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The inadequacy of conventional cell‐monolayer planar cultures and animal experiments in predicting the toxicity and clinical efficacy of drug candidates has led to an imminent need for in vitro methods with the ability to better represent in vivo conditions and facilitate the systematic investigation of drug candidates. Recent advances in 3D bioprinting have prompted the precise manipulation of cells and biomaterials, rendering it a promising technology for the construction of in vitro tissue/organ models and drug screening devices. This review presents state‐of‐the‐art in vitro methods used for preclinical drug screening and discusses the limitations of these methods. In particular, the significance of constructing 3D in vitro tissue/organ models and microfluidic analysis devices for drug screening is emphasized, and a focus is placed on the grafting process of 3D bioprinting technology to the construction of such models and devices. The in vitro methods for drug screening are generalized into three types: mini‐tissue, organ‐on‐a‐chip, and tissue/organ construct. The revolutionary process of the in vitro methods is demonstrated in detail, and relevant studies are listed as examples. Specifically, the tumor model is adopted as a precedent to illustrate the possible grafting of 3D bioprinting to antitumor drug screening.

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“…[ 18 ] Using a droplet‐based bioprinting, Graham et al generated a renal‐like tissue using human embryonic kidney cells (HEK cells) and mesenchymal stem cells. [ 19 ]…”

Section: Evolution Of In Vitro Methods For Drug Screeningmentioning

confidence: 99%

Grafting of 3D Bioprinting to In Vitro Drug Screening: A Review

Nie

Gao

et al. 2020

Adv Healthcare Materials

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“…Living cells can also be assembled into patterned 3D structures by various processes [31,39,40] including 3D printing in droplets [41]. Similarly, living cells might be included in selected compartments within synthetic tissues.…”

Section: Hybrid Tissuesmentioning

confidence: 99%

Synthetic tissues

Bayley

Hoskin

2019

Emerging Topics in Life Sciences

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While significant advances have been achieved with non-living synthetic cells built from the bottom-up, less progress has been made with the fabrication of synthetic tissues built from such cells. Synthetic tissues comprise patterned three-dimensional (3D) collections of communicating compartments. They can include both biological and synthetic parts and may incorporate features that do more than merely mimic nature. 3D-printed materials based on droplet-interface bilayers are the basis of the most advanced synthetic tissues and are being developed for several applications, including the controlled release of therapeutic agents and the repair of damaged organs. Current goals include the ability to manipulate synthetic tissues by remote signaling and the formation of hybrid structures with fabricated or natural living tissues.

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“…However, the addition of this layer can change the BM biophysical properties and to date there is no proof of BM remodelling. The layerby-layer coculture has two major advantages: (i) its compatibility with bioprinting, which increases the complexity of the culture system by generating a specific pattern with various cell types [145][146][147][148] and (ii) the possibility to use cell sheet engineering to generate a complex sheet of cells thanks to a thermo-sensible polymer. 149,150 This cell sheet method can be used to integrate the cocultured cells in an advanced cell-based assay.…”

Section: D) Extracellular Matrix Protein Gelmentioning

confidence: 99%