Engineered basement membranes: from<i>in vivo</i>considerations to cell-based assays

Perry, Guillaume; Xiao, Wenjin; Welsh, Gavin I.; Perriman, Adam W.; Lennon, Rachel

doi:10.1039/c8ib00138c

Cited by 17 publications

(17 citation statements)

References 165 publications

(182 reference statements)

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“…Nevertheless, advances in chemistry, materials science and nanotechnology have greatly improved the fabrication of surfaces with mechanical properties and nano-to micro-scale topographical patterns with features that somewhat resemble the native BM. Three main families of engineered BMs are summarized here; more detailed descriptions can be found elsewhere (Perry et al, 2018).…”

Section: Unidirectional Topographymentioning

confidence: 99%

The basement membrane as a structured surface – role in vascular health and disease

Leclech

Natale

Barakat

2020

Journal of Cell Science

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The basement membrane (BM) is a thin specialized extracellular matrix that functions as a cellular anchorage site, a physical barrier and a signaling hub. While the literature on the biochemical composition and biological activity of the BM is extensive, the central importance of the physical properties of the BM, most notably its mechanical stiffness and topographical features, in regulating cellular function has only recently been recognized. In this Review, we focus on the biophysical attributes of the BM and their influence on cellular behavior. After a brief overview of the biochemical composition, assembly and function of the BM, we describe the mechanical properties and topographical structure of various BMs. We then focus specifically on the vascular BM as a nano- and micro-scale structured surface and review how its architecture can modulate endothelial cell structure and function. Finally, we discuss the pathological ramifications of the biophysical properties of the vascular BM and highlight the potential of mimicking BM topography to improve the design of implantable endovascular devices and advance the burgeoning field of vascular tissue engineering.

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Section: Unidirectional Topographymentioning

confidence: 99%

The basement membrane as a structured surface – role in vascular health and disease

Leclech

Natale

Barakat

2020

Journal of Cell Science

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“…However, the success of current replacement structures is limited by poor re‐epithelialization 6 . To regenerate airway epithelium, one reconstruction strategy uses mimicking components of the native basement membrane which structurally underlies epithelial tissue, anchors epithelial cells, and acts as a barrier between tissue compartments 7 . Native basement membrane predominantly consists of extracellular matrix proteins including collagen IV, which maintains the strength and function of the membrane, and laminin, which is the most abundant non‐collagenous glycoprotein.…”

Section: Introductionmentioning

confidence: 99%

Regenerating airway epithelium using fibrous biomimetic basement membranes

Gadalla

Tchoukalova

Lott

2022

J Biomedical Materials Res

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There are reciprocal interactions between epithelial cells and underlying basement membrane. The resemblance of biomaterials to native basement membrane is thus critical for their success when used to regenerate epithelium-containing organs. Particularly, the use of nanofibers and the incorporation of basement membrane proteins may mimic both biophysical and biochemical properties of basement membrane, respectively. Herein we tested how electrospun polycaprolactone/ heparin fibers with and without adsorbed laminin and collagen IV proteins affect epithelial cell functions. We found that airway epithelial cells attached, migrated, and proliferated on all scaffolds but protein-functionalized fibers promoted higher attachment, quicker migration, and increased proliferation. Fibers were then integrated on polyethylene scaffolds and cultured at an air-liquid interface. The detection of secretory and ciliated cell markers was higher in cells on polyethylene with fibers. These findings demonstrate that electrospun fibers incite beneficial epithelial cell responses and can be used in the fabrication of bioengineered functional epithelia.

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“…The basement membrane is a thin sheet-like extracellular matrix (ECM) mainly composed of collagen type IV and laminin networks that underlie epithelial and endothelial cell layers, which play important roles in structural support, building a barrier between tissue compartments, and maintaining the cell phenotypes regulated by cell-ECM interactions. It actively interacts with the cells in a bidirectional manner through the cell surface receptors to create functional homeostasis of a specific tissue [3]. To mimic the in vivo basement membrane in MPS, semipermeable polymer membranes have been popularly used due to their simplicity to fabricate and relatively strong mechanical property.…”

Section: Introductionmentioning

confidence: 99%

Condensed ECM-based nanofilms on highly permeable PET membranes for robust cell-to-cell communications with improved optical clarity

Choi

Jin

et al. 2021

Biofabrication

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The properties of a semipermeable porous membrane, including pore size, pore density, and thickness, play a crucial role in creating a tissue interface in a microphysiological system (MPS) because it dictates multicellular interactions between different compartments. The small pore-sized membrane has been preferentially used in an MPS for stable cell adhesion and the formation of tissue barriers on the membrane. However, it limited the applicability of the MPS because of the hindered cell transmigration via sparse through-holes and the optical translucence caused by light scattering through pores. Thus, there remain unmet challenges to construct a compartmentalized MPS without those drawbacks. Here we report a submicrometer-thickness (∼500 nm) fibrous extracellular matrix (ECM) film selectively condensed on a large pore-sized track-etched (TE) membrane (10 µm-pores) in an MPS device, which enables the generation of functional tissue barriers simultaneously achieving optical transparency, intercellular interactions, and transmigration of cells across the membrane. The condensed ECM fibers uniformly covering the surface and 10 µm-pores of the TE membrane permitted sufficient surface areas where a monolayer of the human induced pluripotent stem cell-derived brain endothelial cells is formed in the MPS device. The functional maturation of the blood–brain barrier (BBB) was proficiently achieved due to astrocytic endfeet sheathing the brain endothelial cells through 10 µm pores of the condensed-ECM-coated TE (cECMTE) membrane. We also demonstrated the extravasation of human metastatic breast tumor cells through the human BBB on the cECMTE membrane. Thus, the cECMTE membrane integrated with an MPS can be used as a versatile platform for studying various intercellular communications and migration, mimicking the physiological barriers of an organ compartment.

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Engineered basement membranes: fromin vivoconsiderations to cell-based assays

Abstract: Engineered basement membranes are required to mimic in vivo properties within cell-based assays.

Cited by 17 publications

References 165 publications

The basement membrane as a structured surface – role in vascular health and disease

The basement membrane as a structured surface – role in vascular health and disease

Regenerating airway epithelium using fibrous biomimetic basement membranes

Condensed ECM-based nanofilms on highly permeable PET membranes for robust cell-to-cell communications with improved optical clarity

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