High resolution NMR spectroscopy of physiological fluids: from metabolism to physiology

Vio-Dury, J.; Nicoli, F.; Torri, Gilbert M; Torri, J.; Kriat, M.; Sciaky, M.; Davin, A.; Viout, Patrick; Confort‐Gouny, S.; Cozzone, Patrick J.

doi:10.1016/0300-9084(92)90062-j

Cited by 16 publications

(7 citation statements)

References 27 publications

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“…The resonances of NeuNAc, GIcNAc and acetate have been measured respectively at 2.06, 2.04 and 1.92 ppm. This spectral attribution was in accordance with data in the published literature: acetate, GIcNAc and NeuNAc presented increasing chemical shifts [18,19,20,21]. The signal intensities were determined from processed spectra and compared with the intensity of the creatinine (ct) resonance at 3.05 ppm.…”

Section: Methodssupporting

confidence: 66%

N-Acetyl Functions and Acetate Detected by Nuclear Magnetic Resonance Spectroscopy of Urine to Detect Renal Dysfunction following Aminoglycoside and/or Glycopeptide Antibiotic Therapy

Racine

Toumelin

Adnet³

et al. 2004

Nephron Physiol

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Background/Aims: N-acetylneuraminidine (NeuNAc), N-acetylglutamine (GIcNAc) and acetate are metabolites present in normal urine. In patients treated with aminoglycosides and/or glycopeptides, elevation of these metabolites in urine suggests renal tubular injury. NeuNAc, GIcNAc and acetate are easily detected by magnetic resonance spectroscopy (MRS), in contrast to other bioanalytical methods. In the present study, these urinary metabolites were detected using MRS and compared with standard biochemical markers of renal injury in intensive care unit patients treated with aminoglycosides and/or glycopeptides. Methods: 16 patients with clinical and biochemical signs of renal dysfunction were included in the study. Proton magnetic resonance spectra were obtained from 134 urine samples. The resonance intensity of NeuNAc, GIcNAc and acetate were reported relative to the resonance intensity of creatinine (ct). These ratios were compared with classical parameters of renal dysfunction, such as plasma creatinine and urea concentration, and 24-hour urine volume, by logistic regression and general linear models. Results: Statistical analysis showed that changes in plasma creatinine and urea concentration were reliably reflected in changes in the NeuNAc/ct ratio, and that plasma urea concentration changes also correlated with the acetate/ct ratio; however, the GIcNAc/ct ratio was not related to these measures of overall renal function. Conclusions: NeuNAc/ct may be a useful marker of renal dysfunction in patients treated with aminoglycosides and glycopeptides; by MRS it can be both straightforward and informative to follow the renal function of patients treated with these antibiotics.

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Section: Methodssupporting

confidence: 66%

N-Acetyl Functions and Acetate Detected by Nuclear Magnetic Resonance Spectroscopy of Urine to Detect Renal Dysfunction following Aminoglycoside and/or Glycopeptide Antibiotic Therapy

Racine

Toumelin

Adnet³

et al. 2004

Nephron Physiol

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“…In addition, this technique can be successfully used to distinguish a large variety of organic components in different corporal fluids (21,22), and it has been widely used in metabolomic studies during the last 20 years. Some of these studies emphasise the research of biomarkers that have metabolic roles that make them useful as indicators of the state and progression of various diseases and of the response to therapeutic treatments, among others.…”

Section: Introductionmentioning

confidence: 99%

NMR metabolic profile of human follicular fluid

et al. 2010

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The environment of the oocyte during its in vivo maturation consists of follicular fluid (FF) and is surrounded by granulosa cells. The FF is derived from the sanguineous plasma and secretions, synthesised in the follicle wall, that contain a large variety of growth factors, cytokines, amino acids, and other metabolites. These metabolites are presumably involved in the physiology of the oocyte. The identification, quantification and study of FF metabolites can provide additional information about the oocyte state which can be helpful in distinguishing those oocytes that have a greater capacity to be fertilised and to develop properly. The aim of this work is to identify the metabolic profile of FF samples exhaustively using High Resolution Nuclear Magnetic Resonance (NMR). A total of 30 FF samples from oocyte donors (<35 years) were analysed. Different monodimensional (1D) and bidimensional (2D) (homo and heteronuclear) NMR experiments were acquired. A total of 131 chemical shifts were assigned and 42 metabolites, including as example glucose, lactate, acetate, acetoacetate, pyruvate and b-hydroxybutyrate, were identified. High correlations were found between these important intermediaries of the energetic metabolic pathways of the follicle which can indicate the importance of these pathways in oocyte development. Some of these identified metabolites might be useful as biomarkers of the follicular maturation state, allowing oocytes with a higher fertilisation potential to be selected, thereby increasing pregnancy rates in women following in vitro fertilisation (IVF) treatments.

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“…Nuclear magnetic resonance spectroscopy (NMR) has played an important role in the growth of this field, aiding in the identification of drug metabolites [2] and disease biomarkers [3] in fluids such as whole blood, plasma, urine, bile, and cerebral spinal fluid [4][5][6]. NMR relaxation and diffusion-based gradient filtering methods have been widely employed to analyze these samples.…”

Section: Introductionmentioning

confidence: 99%

Progress toward automated metabolic profiling of human serum: Comparison of CPMG and gradient-filtered NMR analytical methods

Lucas¹,

Larive²,

Wilkinson³

et al. 2005

Journal of Pharmaceutical and Biomedical Analysis

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High resolution NMR spectroscopy of physiological fluids: from metabolism to physiology

Cited by 16 publications

References 27 publications

N-Acetyl Functions and Acetate Detected by Nuclear Magnetic Resonance Spectroscopy of Urine to Detect Renal Dysfunction following Aminoglycoside and/or Glycopeptide Antibiotic Therapy

N-Acetyl Functions and Acetate Detected by Nuclear Magnetic Resonance Spectroscopy of Urine to Detect Renal Dysfunction following Aminoglycoside and/or Glycopeptide Antibiotic Therapy

NMR metabolic profile of human follicular fluid

Progress toward automated metabolic profiling of human serum: Comparison of CPMG and gradient-filtered NMR analytical methods

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