Progress toward automated metabolic profiling of human serum: Comparison of CPMG and gradient-filtered NMR analytical methods

Lucas, Laura H.; Larive, Cynthia K.; Wilkinson, Patricia Stone; Huhn, Stephen D.

doi:10.1016/j.jpba.2004.09.060

Cited by 32 publications

(16 citation statements)

References 31 publications

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“…The spectra were obtained using a CPMGPR1D pulse sequence (Bruker Biospin, Germany), which was used to attenuate the broad protein signal in the plasma. This sequence is a modification of the Carr-Purcell-Meiboom-Gill pulse sequence (CPMGpulse, Bruker Biospin Germany) that suppresses the residual water signal (Lucas et al 2005).…”

Section: H Nmr Spectroscopymentioning

confidence: 99%

Effects of vitamin B12 on murine embryonic dexamethasone-induced cleft palates using 1H-NMR-based metabonomic analysis

Zhou

Huang

et al. 2010

Metabolomics

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Metabonomics using proton nuclear magnetic resonance ( 1 H-NMR) spectroscopy and multivariate data analysis of blood plasma samples can be used to characterize metabolic differences between healthy and diseased organisms, which can reveal important information about the causes of the disease. Here we evaluated whether the 1 H-NMR-based metabonomic method can detect differences in blood plasma between healthy pregnant mice (outbred C57BL/6J strain) and pregnant mice injected with dexamethasone (Dex) to induce cleft palate. Both groups were injected with vitamin B 12 . We found some metabolic differences from the ''outliers'' among the mice, indicating that vitamin B 12 protected against Dex-induced Cleft lip with or without palate (CLP) formation.

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Section: H Nmr Spectroscopymentioning

confidence: 99%

Effects of vitamin B12 on murine embryonic dexamethasone-induced cleft palates using 1H-NMR-based metabonomic analysis

Zhou

Huang

et al. 2010

Metabolomics

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“…To probe interaction mechanisms and to study the phases separately, spectral editing methods are extremely useful. These procedures involve exploiting differences in properties such as relaxation and diffusion to separate gel-like and dissolved molecules [25][26][27]. For instance, the inverse diffusion editing experiment (iDE) [27] is based on the application of single PGSE (pulsed gradient spin echo) that allows the separation of different motional regimes present in complex mixtures.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive multiphase NMR spectroscopy: A new analytical method to study the effect of biodiesel blends on the structure of commercial rubbers

Silva

Filho

Simpson

et al. 2016

Fuel

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h i g h l i g h t sWe evaluate the effect of biodiesel on commercial rubber at molecular level. The new CMP-NMR probe was successful to study the phases in the fuel-rubber system. We observed that the biodiesel penetrates at EPDM despite the opposite report. It is observed the increasing on rubbers softening after biodiesel immersion. a b s t r a c t Biodiesel has been incorporated into the energy matrix in several countries, but its compatibility with some materials used in automotive engines is of growing concern. In the present study, comprehensive multiphase (CMP) NMR is applied to understand the different phases in fuel-rubber systems. CMP NMR is a novel technology that integrates all the hardware from solution-, gel-and solid-state into a single NMR probe, permitting all phases to be studied in samples in their natural unaltered state. Transverse relaxation experiments in combination with inverse diffusion editing permit the increasing mobility of the rubbers chains with biodiesel exposure to be monitored. Conversely diffusion editing and RADE experiments highlight the more rigid domains. In summary NR and NBR showed the absence of highly rigid domains after exposure to biodiesel whereas SBR and EPDM especially better retained their structural integrity. 13 C editing protocols and 13 C-1 H HSQC confirmed the increasing gel-like properties of NR, NBR and SBR with exposure to biodiesel. However, variable contact time experiments showed that biodiesel penetrates even the most resilient EPDM pores causing relaxation of the polymer chains and demonstrates that NMR is sensitive enough to highlight even the very slight swelling of the EPDM. Through these results it is possible to observe that the elastomer that exhibits the lowest susceptibility to biodiesel in molecular terms was EPDM, followed by SBR, NR, and NBR. It was also observed that biodiesel molecules were present in the EPDM structure despite the literature reporting its general resilience to biodiesel.

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“…It uses analytic technologies, such as capillary electrophoresis [7], Fourier-transform infrared spectroscopy [8], electrochemistry [9], mass spectrometry (MS) [10][11][12] and nuclear magnetic resonance (NMR) [13][14][15], to identify and quantify small metabolites in biofluids or tissues, and it uses pattern-recognition approaches and bioinformatics to relate this metabolic information to pathophysiological processes [16,17]. Among them, several aspects of NMR spectroscopy have made it a useful tool in metabonomics for 20 years: it is non-destructive, which allows samples to remain in physiological conditions; it provides information about dynamic processes, it permits unbiased metabolite detection and it is quantitative and reproducible [18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

NMR-based metabonomics: a useful platform of oncology research

2010

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Cancer threatens human health, thus research focusing on oncology has great significance. Metabonomics is the global quantitative assessment of the dynamic metabolic response of a biological system to some exogenous or genetic pathophysiological perturbation. The metabolites are detected in tissues or fluids by various analytical methods, such as nuclear magnetic resonance (NMR) and mass spectroscopy. Metabonomics, as a tool, can provide a link between the laboratory and clinic. NMRbased metabonomics offers a useful tool to understand tumour biochemistry and may also has some potentials for tumour diagnosis and prognosis, even when some other disease processes are present. Here, we review NMR-based metabonomics principles and their applications in oncology research.

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Progress toward automated metabolic profiling of human serum: Comparison of CPMG and gradient-filtered NMR analytical methods

Cited by 32 publications

References 31 publications

Effects of vitamin B12 on murine embryonic dexamethasone-induced cleft palates using 1H-NMR-based metabonomic analysis

Effects of vitamin B12 on murine embryonic dexamethasone-induced cleft palates using 1H-NMR-based metabonomic analysis

Comprehensive multiphase NMR spectroscopy: A new analytical method to study the effect of biodiesel blends on the structure of commercial rubbers

NMR-based metabonomics: a useful platform of oncology research

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