High-resolution microscopy of the implant-tissue interface

Osseointegration of metallic implants: II. Transmission electron microscopy in the rabbit

“…The histologic method used in this study is based on in situ embedding oftissueandimplant andsubsequent separation of embedment and implant (Linder 1985). The latter procedure has been shown to entail a potential loss of < 10 nm of interface tissue, which should be acceptable even for TEM studies (Lausmaa and Linder 1988).…”

“…The method of implant-embedment separation (Linder 1985) may theoretically entail a loss of up to 10 nm of plastic from the embedment (Lausmaa and Linder 1988). Because the edge of our tissue sections in many cases was indistinct and darkstaining, a reliable quantification was not possible.…”

Osseointegration of bone implants: A review of an alternative mode of fixation

“…Such different techniques in which the true interfacial structures can be studied with the electron microscope have been presented by Albrektsson et al (1982), Linder (1985), and Thomsen and Ericson (1985). Presumably, it may be possible to relate different interfacial phenomena to tissue integration when the tissue reactions are studied at the ultrastructural level instead of with low-power light microscopy.…”

Histological and ultrastructural evaluation of bone around Bio‐Oss® particles in sinus augmentation

“…Light microscopy (LM) provides the most important information about the presence of bone or soft tissue contact, but it does not give ultrastructural information about the organization at the interface (Linder, 1985; Ganeles et al , 1986; De Lange and Donath, 1989; Steflik et al , 1989; Davies et al , 1990; Van Blitterswijk et al , 1990; Sennerby et al , 1991). In transmission electron microscopy (TEM) studies, an electron‐dense granular layer at the interface with hydroxyapatite (HA) and titanium has been reported (Van Blitterswijk et al , 1990; Sennerby et al , 1991).…”