High-resolution mapping, cloning and molecular characterization of the Pi-k
                     
                h
               gene of rice, which confers resistance to Magnaporthe grisea

Sharma, Tilak Raj; Madhav, M. S.; Singh, Balwant; Shanker, P.; Jana, Tarakanta; Dalal, Vivek; Pandit, Awadhesh; Singh, A. K.; Gaikwad, Kishor; Upreti, H. C.; Singh, Nirupma

doi:10.1007/s00438-005-0066-8

Cited by 41 publications

(75 citation statements)

References 23 publications

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“…To reduce false positives, only nodes with a duplication consistency score 129 greater than 0.5 were collected. To map published disease resistance genes to R-gene families, the following GenBank protein fasta accessions were used as queries: BAK39922.1 (RGA4) 78 159 , AAY33493.1 (pi54) 160 , CCD32366.1 (pi54), BAG72135.1 (Pik1) 83 , BAG72136.1 (Pik2) 83 , ABA92370.1 (NLS1) 161 , AAO45178.1 (Pi-ta) 161 , XP_015626818 (RLS1) 162 and AB002266 (Xa1) 163 . NCBI BLASTP 164 was performed, and significant hits (E value ≤ 1 × 10 -10 ) were manually evaluated for quality and assigned to a gene family.…”

Section: Competing Interestsmentioning

confidence: 99%

Genomes of 13 domesticated and wild rice relatives highlight genetic conservation, turnover and innovation across the genus Oryza

et al. 2018

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Section: Competing Interestsmentioning

confidence: 99%

Genomes of 13 domesticated and wild rice relatives highlight genetic conservation, turnover and innovation across the genus Oryza

et al. 2018

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“…Simple InDels and Single Nucleotide Polymorhisms (SNPs) have shown to play a pivotal role in R-gene evolution through selection (Shen et al 2006). Differences in single nucleotide in a regulatory region of Pi54 locus distinguish resistant phenotype from the susceptible one (Sharma et al 2005). The presence of 5 Mb region (superlocus) physically linked to Pi-ta gene impart Figure 3.…”

Section: Discussionmentioning

confidence: 99%

Positive selection pressure on rice blast resistance allelePiz-tmakes it divergent in Indian land races

Thakur¹,

Singh²,

Rathour³

et al. 2013

Journal of Plant Interactions

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Allelic variants of the broad-spectrum blast resistance gene, Piz-t, have been analyzed in 48 rice lines selected after phenotyping across three blast hot-spot regions of India. Single Nucleotide Polymorhisms in the form of transitions were more frequent than the transversions in the alleles. On the basis of nucleotide polymorphism, 46 haplotypes have been identified, with major haplotypes forming three main haplogroups. The Piz-t alleles formed mostly region-specific clusters. Resistant and susceptible Piz-t alleles were grouped into separate sub-clusters. The value of Tajima's D was negative indicating positive selection on Piz-t locus. Sequence variations were more abundant in the leucine rich repeats (LRR) region than in the NB-ARC (nucleotide-binding adaptor shared by APAF-1,R proteins, and CED-4) region, indicating that the LRR region has played a more important role in the evolution of this allele. The detailed molecular analysis of the Piz-t locus provide insights to high degree of interand intra-specific relationship among the Indian land races of rice which will help in the selection of better alleles for future rice breeding programs.

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“…Although this result is consistent with the notion that these three genes are multiple alleles at the one locus, more detailed analysis is necessary to confirm that they are allelic. Sharma et al 90 Based on the data of genome-wide mapping of the NBS-LRR domain containing genes reported by Monosi et al 72 , all three clusters of the rice resistance genes are closely associated with the clusters of NBS-LRR domain 2): C and P indicate complete resistance and partial resistance, respectively. The classifications of resistance pattern of each gene were followed according to Koizumi (2007) and the references for each gene (C: compete resistance, P: partial resistance).…”

Section: Location Of the Resistance Genesmentioning

confidence: 99%

Resistance Genes and Selection DNA Markers for Blast Disease in Rice (Oryza sativa L.)

Koide

Kobayashi

et al. 2009

JARQ

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Blast is a serious disease caused by a fungal pathogen Pyricularia oryzae Cavara of rice (Oryza sativa L.). The use of resistant varieties is considered one of the most efficient ways of crop protection from the disease. In addition to a large amount of information accumulated during the long history of genetic studies on resistance to rice blast, recent progress in rice genomics has enabled us to use DNA markers for breeding the resistant varieties by marker assisted selection (MAS). In this report, we summarize the reported rice blast resistance genes and their selection markers to encourage further utilization for breeding. First, we assemble the information about the reported genes with regard to their number, chromosomal locations, patterns of resistance, donor strains, and molecular characterization of the cloned genes by reviewing the literature. In addition, we present some remaining issues about the nomenclature system and identification of the resistance genes. Then, we provide the first assembled list of the reported DNA markers for blast resistance genes, including the sequences of the primer pairs, genetic distances from the resistance genes, and cross combinations of the parental strains used to detect the polymorphisms. This information will help rice breeders to improve the resistance to rice blast by MAS.

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High-resolution mapping, cloning and molecular characterization of the Pi-k h gene of rice, which confers resistance to Magnaporthe grisea

Abstract: The online version of the original article can be found at http:// dx

Cited by 41 publications

References 23 publications

Genomes of 13 domesticated and wild rice relatives highlight genetic conservation, turnover and innovation across the genus Oryza

Genomes of 13 domesticated and wild rice relatives highlight genetic conservation, turnover and innovation across the genus Oryza

Positive selection pressure on rice blast resistance allelePiz-tmakes it divergent in Indian land races

Resistance Genes and Selection DNA Markers for Blast Disease in Rice (Oryza sativa L.)

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