High‐resolution in vivo CBV mapping with MRI in wild‐type mice

Wu, Ed X.; Wong, Kelvin; Andrassy, Martin; Tang, Haiying

doi:10.1002/mrm.10425

Cited by 44 publications

(44 citation statements)

References 20 publications

(31 reference statements)

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“…advise 5-15 mgFe/kg to measure CBV. Wu et al [35] measured microvascular volume distribution in mice using a 30 mgFe/kg injection at 9.4 T and demonstrated that CBV in the cerebral cortex is higher in transgenic APP mice, an experimental model of Alzheimer's disease, than in controls. CBV-weighted functional MRI (fMRI) has been extensively used in experimental fMRI [36,37]; thanks to the increased sensitivity of CBV-weighted contrast compared with classical blood oxygen level-dependent (BOLD) contrast.…”

Section: Discussionmentioning

confidence: 99%

Polymer-Coated Superparamagnetic Iron Oxide Nanoparticles as T ₂ Contrast Agent for MRI and their Uptake in Liver

et al. 2017

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Aim:To study the efficiency of multifunctional polymer-based superparamagnetic iron oxide nanoparticles (bioferrofluids) as a T 2 magnetic resonance contrast agent and their uptake and toxicity in liver. Materials & methods: Mice were intravenously injected with bioferrofluids and Endorem R . The magnetic resonance efficiency, uptake and in vivo toxicity were investigated by means of magnetic resonance imaging (MRI) and histological techniques. Results: Bioferrofluids are a good T 2 contrast agent with a higher r 2 /r 1 ratio than Endorem. Bioferrofluids have a shorter blood circulation time and persist in liver for longer time period compared with Endorem. Both bioferrofluids and Endorem do not generate any noticeable histological lesions in liver over a period of 60 days post-injection. Conclusion: Our bioferrofluids are powerful diagnostic tool without any observed toxicity over a period of 60 days post-injection.Lay abstract: Several superparamagnetic iron oxide nanoparticles (SPIONs) preparations have been approved by US FDA for clinical use as MRI contrast agents. In recent years, we have been developing a synthetic multifunctional platform for SPIONs based on the use of polymers. In this report, we explored the diagnostic potential of these nanoparticles (herein called bioferrofluids) as an MRI contrast agent and their uptake in liver, without neglecting their toxicological effects. Results show that our bioferrofluids are a good T 2 contrast agent without any observed toxicity in liver.

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Section: Discussionmentioning

confidence: 99%

Polymer-Coated Superparamagnetic Iron Oxide Nanoparticles as T ₂ Contrast Agent for MRI and their Uptake in Liver

et al. 2017

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“…Some rodent fMRI studies have also utilized SPIO/ultrasmall (US)PIO contrast materials to enhance signal loss at the activated areas [51]. These techniques also allow the measurement of cerebral blood volume [51,56,57]. One recent fMRI study was the first such study to successfully utilize thermal stimuli to distinguish the noxious and innocuous pathways in the rat spinal cord [58].…”

Section: Functional Mri Studiesmentioning

confidence: 99%

“…Another important aspect is the N-acetyl-aspartate peak that may show a decrease in completed strokes, but may remain normal if the animals still can recover from the ischemic event [124]. Magnetic resonance angiography [125] and perfusion-weighted imaging [57] have been performed and evaluated in several stroke models. These studies accurately characterize the cerebral blood flow and blood volume during ischemia.…”

Section: Stroke Modelsmentioning

confidence: 99%

MRI in Rodent Models of Brain Disorders

et al. 2011

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Summary: Magnetic resonance imaging (MRI) is a wellestablished tool in clinical practice and research on human neurological disorders. Translational MRI research utilizing rodent models of central nervous system (CNS) diseases is becoming popular with the increased availability of dedicated small animal MRI systems. Projects utilizing this technology typically fall into one of two categories: 1) true "pre-clinical" studies involving the use of MRI as a noninvasive disease monitoring tool which serves as a biomarker for selected aspects of the disease and 2) studies investigating the pathomechanism of known human MRI findings in CNS disease models. Most small animal MRI systems operate at 4.7-11.7 Tesla field strengths. Although the higher field strength clearly results in a higher signalto-noise ratio, which enables higher resolution acquisition, a variety of artifacts and limitations related to the specific absorption rate represent significant challenges in these experiments. In addition to standard T1-, T2-, and T2*-weighted MRI methods, all of the currently available advanced MRI techniques have been utilized in experimental animals, including diffusion, perfusion, and susceptibility weighted imaging, functional magnetic resonance imaging, chemical shift imaging, heteronuclear imaging, and 1 H or 31 P MR spectroscopy. Selected MRI techniques are also exclusively utilized in experimental research, including manganese-enhanced MRI, and cell-specific/molecular imaging techniques utilizing negative contrast materials. In this review, we describe technical and practical aspects of small animal MRI and provide examples of different MRI techniques in anatomical imaging and tract tracing as well as several models of neurological disorders, including inflammatory, neurodegenerative, vascular, and traumatic brain and spinal cord injury models, and neoplastic diseases.

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“…Some rodent fMRI studies also used SPIO/USPIO contrast materials to enhance signal loss at the activated areas, 36 these techniques also allow the measurement of cerebral blood volume. 36,38 Immune-mediated diseases of the CNS are frequently studied in animal models. The most common models include experimental allergic encephalomyelitis (EAE), Theiler's Murine Encephalitis Virus infection (EV) and toxic demyelination models.…”

Section: Figmentioning

confidence: 99%

“…62,63,66 Another important aspect is the NAA peak that may show decrease in completed strokes, but may remain normal if the animals still can recover from the ischemic event. 67 MRA 68 and cerebral blood volume/cerebral blood flow studies 38 have been done and evaluated in several stroke models. These studies can accurately characterize the blood flow and blood volume during ischemia.…”

Section: Figmentioning

confidence: 99%

Magnetic resonance imaging, microscopy, and spectroscopy of the central nervous system in experimental animals

et al. 2005

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Summary:Over the last two decades, microscopic resolution in vivo magnetic resonance imaging (MRI) techniques have been developed and extensively used in the study of animal models of human diseases. Standard MRI methods are frequently used in clinical studies and in the general clinical practice of human neurological diseases. This generates a need for similar studies in experimental animal research. Because small rodents are the most commonly used species as animal models of neurological diseases, the MRI techniques need to be able to provide microscopic resolution and high signal-to-noise ratio images in relatively short time. Small animal MRI systems use very high field-strength magnets, which results in higher signal to noise ratio; however, the contrast characteristics of live tissue are different at these field strengths. In addition to standard MRI techniques, several new applications have been implemented in experimental animals, including diffusion and perfusion studies, MR angiography, functional MRI studies, MRI tractography, proton and phosphorous spectroscopy, cellular and molecular imaging using novel contrast methods. Here we give an overview of how to establish a small animal imaging facility with the goal of CNS imaging. We describe the basic physical processes leading to MR signal generation, highlighting the differences between standard clinical MRI and small animal MRI. Finally, typical findings in the most common neurological disease categories and novel MRI/magnetic resonance spectroscopy methods used in their study are also described.

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High‐resolution in vivo CBV mapping with MRI in wild‐type mice

Cited by 44 publications

References 20 publications

Polymer-Coated Superparamagnetic Iron Oxide Nanoparticles as T ₂ Contrast Agent for MRI and their Uptake in Liver

Polymer-Coated Superparamagnetic Iron Oxide Nanoparticles as T ₂ Contrast Agent for MRI and their Uptake in Liver

MRI in Rodent Models of Brain Disorders

Magnetic resonance imaging, microscopy, and spectroscopy of the central nervous system in experimental animals

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High‐resolution in vivo CBV mapping with MRI in wild‐type mice

Cited by 44 publications

References 20 publications

Polymer-Coated Superparamagnetic Iron Oxide Nanoparticles as T 2 Contrast Agent for MRI and their Uptake in Liver

Polymer-Coated Superparamagnetic Iron Oxide Nanoparticles as T 2 Contrast Agent for MRI and their Uptake in Liver

MRI in Rodent Models of Brain Disorders

Magnetic resonance imaging, microscopy, and spectroscopy of the central nervous system in experimental animals

Contact Info

Product

Resources

About

Polymer-Coated Superparamagnetic Iron Oxide Nanoparticles as T ₂ Contrast Agent for MRI and their Uptake in Liver

Polymer-Coated Superparamagnetic Iron Oxide Nanoparticles as T ₂ Contrast Agent for MRI and their Uptake in Liver