High-resolution imaging of a cell-attached nanointerface using a gold-nanoparticle two-dimensional sheet

Masuda, Shihomi; Yanase, Yuhki; Usukura, Eiji; Ryuzaki, Sou; Wang, Pangpang; Okamoto, Koichi; Kuboki, Thasaneeya; Kidoaki, Satoru; Tamada, Kaoru

doi:10.1038/s41598-017-04000-4

Cited by 39 publications

(45 citation statements)

References 54 publications

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“…Rat basophilic leukaemia (RBL-2H3) cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% foetal calf serum (FCS), 100 U/mL penicillin, and 100 μg/mL streptomycin [ 6 ]. The day before the experiment, RBL-2H3 cells were harvested using trypsin.…”

Section: Methodsmentioning

confidence: 99%

“…The RBL-2H3 cells in RPMI 1640 medium were then placed into the well and cultured for the indicated time (1 or 2 hours or overnight) in a CO 2 incubator (4% CO 2 concentration) at 37°C. The detailed procedure was described in our previous paper [ 6 ].…”

Section: Methodsmentioning

confidence: 99%

“…In our previous study, we proposed a simple, effective method for visualizing the nanointerface of adhesive cells using localized surface plasmon resonance (LSPR) excited on a two-dimensional (2D) self-assembled gold nanoparticle (NP) sheet[ 6 ]. The light confined by LSPR detects fluorescence in a region of only a few tens of nanometres at the interface, which creates notably high ‘axially’ confined imaging that is superior to any other super-resolution microscopic techniques, including TIRF microscopy[ 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…However, the propagating property often deteriorates the spatial resolution of the images. Unlike the ‘propagating’ SPR-mediated imaging, our LSPR technique exhibits extremely high axial confinement and practically high lateral resolution because there is little overlap of the excited fluorescence molecules in the depth direction[ 6 ]. In this paper, we utilized a silver nanoparticle sheet for this application[ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

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LSPR-mediated high axial-resolution fluorescence imaging on a silver nanoparticle sheet

et al. 2017

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This paper reports our original technique for visualizing cell-attached nanointerfaces with extremely high axial resolution using homogeneously excited localized surface plasmon resonance (LSPR) on self-assembled silver nanoparticle sheets. The LSPR sheet can confine and enhance the fluorescence at the nanointerface, which provides high signal-to-noise ratio images of focal adhesion at the cell-attached interface. The advantage of this LSPR-assisted technique is its usability, which provides comparable or higher-quality nanointerfacial images than TIRF microscopy, even under epifluorescence microscopy. We also report the cytotoxicity of silver nanoparticles, as determined via morphological analysis of adherent cells on the sheet.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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LSPR-mediated high axial-resolution fluorescence imaging on a silver nanoparticle sheet

et al. 2017

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“…Although the detection limit of a confocal laser scanning microscope in z direction is known to be approx. 500 nm [9], some holographic microscopes can observe almost 30 m in depth from the cellular top surface. Taking advantage of these techniques, the cell-labeling nanomaterials can be utilized for immunoassay to diagnose early stage cancer in the visible light region.…”

Section: Introductionmentioning

confidence: 99%

Design of Hydroxyapatite-Based Multifunctional Nanoparticles for Cell Labelling and Cell Growth Inhibition

2019

Regen Med Front.

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There has been an increasing demand for the development of cell-labeling nanomaterials that safely label and visualize a specific type of cells for diagnosis and inspection in vivo and in vitro. In order to design such cell-labeling nanomaterials, the properties of efficient visible light luminescence and effective interactions with cells have to be realized using a biocompatible nanomaterial. From this viewpoint, we summarize and overview the current situation on cell-labeling technologies. Among various functional nanomaterials, we focus on hydroxyapatite nanoparticles and their photofunctionalization based on the properly designed inorganic-organic hybrid structure such as hydroxyapatite/ organic europium (III) complex. Also, the immobilization technique of a specific binding molecule to the solid surface is introduced to demonstrate the selective uptake into cancer cells. Moreover, an example of the growth inhibitory drug molecules for cancer cells are described, focusing on the cytostatic inhibition of citric acid and the potential use of hydroxyapatite/citric acid hybrids. Finally, we mention our future perspectives on the theranostic nanoparticles with fluorescence and therapeutic properties that are achieved through the hydroxyapatiteorganic hybrid interfacial interactions.

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Metal‐Enhanced Fluorescence and Its Applications

Sugawa¹

2018

Encyclopedia of Analytical Chemistry

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The fluorescence‐enhancement phenomenon of fluorophores positioned near metal nanostructures (metal‐enhanced fluorescence, MEF) is the result of the interaction between the fluorophores and the localized surface plasmon resonance (LSPR) of the nanostructures. This enhancement phenomenon can contribute to the development of novel sensors based on unprecedented mechanisms, as well as improving the sensitivity of existing biosensors and imaging techniques. In this article, the two main mechanisms for MEF are briefly explained: the light‐harvesting nanoantenna effect and the increase in the radiative decay rate. This is followed by an introduction to metal nanostructures and nanoparticles (substrate‐based platforms and solution‐dispersed colloidal nanoparticle‐based platforms), which can act as MEF platforms. In addition, the efficient suppression of fluorescence blinking and photobleaching induced by the interaction with LSPR are described, as these are secondary advantages for fluorescence‐based sensors. Subsequently, the generation of hot spots, which could be one of the key phenomena for significant fluorescence enhancement, is outlined. An examination of usability of MEF platforms consisting of Al and Cu (metal species other than Au and Ag) to further improve their applicability is also discussed. Finally, some recent applications such as the development of imaging techniques, nanoprobes, and biosensors utilizing the MEF phenomenon are briefly introduced.

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High-resolution imaging of a cell-attached nanointerface using a gold-nanoparticle two-dimensional sheet

Cited by 39 publications

References 54 publications

LSPR-mediated high axial-resolution fluorescence imaging on a silver nanoparticle sheet

LSPR-mediated high axial-resolution fluorescence imaging on a silver nanoparticle sheet

Design of Hydroxyapatite-Based Multifunctional Nanoparticles for Cell Labelling and Cell Growth Inhibition

Metal‐Enhanced Fluorescence and Its Applications

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