High-resolution/higher-order derivative spectrophotometry for determination of ternary mixtures of B-complex vitamins in pharmaceuticals

Morelli, Basilio

doi:10.1007/s002169600016

Cited by 28 publications

(11 citation statements)

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“…In the mixture under analysis, Dl = 6 nm was the optimum for all determinations except for the measurement of AMD at 1 DD 299. 4 where Dl = 4 nm was applied.…”

Section: Derivative Ratio Methodsmentioning

confidence: 99%

“…The zero-crossing method 1,2 has led to significant developments in the analysis of pharmaceutical compounds in multi-component mixtures. 3,4 In 1990, Salinas et al 5 developed the ratio spectra derivative spectrophotometry for the resolution of binary mixtures. In 1992, Berzas et al 6 developed a method and discussed the theory for resolving ternary mixtures based on the use of first derivative of the ratio spectra of mixtures, followed by measurements at the zero crossing wavelengths of the first derivative of ratio spectra of single components.…”

Section: Introductionmentioning

confidence: 99%

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Derivative and Derivative Ratio Spectrophotometric Analysis of Antihypertensive Ternary Mixture of Amiloride Hydrochloride, Hydrochlorothiazide and Timolol Maleate

Abdel-Hay

Gazy

Hassan

et al. 2008

J Chinese Chemical Soc

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Two simple, rapid and reliable spectrophotometric methods are described for the resolution of the three-component mixture of amiloride hydrochloride (AMD), hydrochlorothiazide (HCT) and timolol maleate (TIM). The first method involves the use of derivative spectrophotometry with the zero-crossing technique where AMD was easily determined using its 0 D and 1 D (Dl = 6) amplitudes at 365 and 385 nm, respectively, while HCT and TIM were determined by measuring the 3 D (Dl = 6) amplitude at 265 nm and the 1 D (Dl = 8) amplitude at 315.4 nm, respectively. The second method involves the application of the ratio-spectra zero-crossing first and second derivative spectrophotometry where two points have been used for the quantification of each compound. For the determination of AMD, HCT was used as divisor and the 1 DD (Dl = 4) and 2 DD (Dl = 6) values at 299.4 and 311 nm, respectively, were plotted against AMD concentration; while -by using TIM as divisor -the 2 DD (Dl = 6) amplitudes at 264.2 and 290 nm were found to be proportional to HCT concentration. TIM was assayed in the mixture using its 1 DD (Dl = 6) amplitudes at 289.8 nm (Divisor was AMD) and 314.8 nm (Divisor was HCT). Synthetic mixtures of different proportions and laboratory-made tablets were assayed by the proposed methods and the results revealed good accuracy and repeatability of the developed methods.

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“…In the mixture under analysis, Dl = 6 nm was the optimum for all determinations except for the measurement of AMD at 1 DD 299. 4 where Dl = 4 nm was applied.…”

Section: Derivative Ratio Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Derivative and Derivative Ratio Spectrophotometric Analysis of Antihypertensive Ternary Mixture of Amiloride Hydrochloride, Hydrochlorothiazide and Timolol Maleate

Abdel-Hay

Gazy

Hassan

et al. 2008

J Chinese Chemical Soc

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“…In addition, the most common requirement for the analysis of VB 12 is in the quality control of pharmaceuticals (tablets or injections), blood plasma serum, milk products for infants and fermentation products in which microorganisms can bond and release VB 12 (1). Presently, there are different methods used for measuring VB 12 concentration, including microbiological assay (2)(3)(4), spectrophotometry (5,6), chemiluminescence (7,8), atomic absorption spectrometry (9), capillary electrophoresis (10), high-performance liquid chromatography (11)(12)(13)(14)(15) and inductive-coupled plasma-mass spectrometry method (16). None of them, however, was very suitable in terms of simplicity, rapidity and accuracy.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of a novel fluorescent probe based on 7‐nitrobenzo‐2‐oxa‐1,3‐diazole skeleton for the rapid determination of vitamin B₁₂ in pharmaceuticals

et al. 2013

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A new fluorescent probe, 4-N,N-di(2-hydroxyethyl)imino-7-nitrobenzo-2-oxa-1,3-diazole (HINBD) was synthesized in a single step with reasonably good yield. The water-soluble HINBD emits strongly in the visible region (λex = 479 nm, λem = 545 nm) and is stable over a wide range of pH values. It was found that vitamin B12 (VB12 ) had the ability to quench the fluorescence of HINBD, and the quenched fluorescence intensity was proportional to the concentration of VB12 . A method for VB12 determination based on the quenching fluorescence of HINBD was thus established. Interference effects of various substances, including sugars, vitamins, amino acids, inorganic cations and some organic substances have been studied. Under optimal conditions, the linear range is 0.0-2.4 × 10(-5) mol/L. The determination limit is 8.3 × 10(-8) mol/L. The method was applied to measure VB12 in pharmaceutical preparations with satisfactory results.

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“…B vitamins have been reported to potentiate the antinociceptive and analgesic effects of diclofenac sodium (DC), and several papers have docu-mented their contributions to reduce daily DC dosage and shorten the treatment time [1][2][3]. Through literature, determinations of B-complex vitamins were carried out, by high-performance liquid chromatography (HPLC) [4][5][6][7][8][9][10][11][12][13], spectrophotometry [14][15][16][17], capillary electrophoresis [18][19] and TLC [20]. Benfotiamine (BN) was only determined in pharmaceutical product in combination with cyanocobalamine and thioctic acid by HPLC [21].…”

Section: Introductionmentioning

confidence: 99%

Application of LC and HPTLC-densitometry for the simultaneous determination of two multicomponent mixtures containing pyridoxine hydrochloride and cyanocobalamine

Hadad

Salam

Hameed

2013

Acta Chromatographica

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Summary. Two methods were presented for the simultaneous determination of two multicomponent mixtures containing pyridoxine hydrochloride (B 6 ) and cyanocobalamine (B 12 ) with benfotiamine (BN) [mix. 1], or thiamine nitrate (B 1 ) and diclofenac sodium (DC) [mix. 2]. The first high-performance liquid chromatographic (HPLC) method depends on the use of a cyanopropyl column at a flow rate of 1.5 mL min −1 with a mobile phase consisting of acetonitrile-25 mM ammonium acetate, pH 3.7 (10:90, v/v), for mix. 1 or acetonitrile-25 mM ammonium acetate, pH 5.0 by gradient elution, changing the proportion of the system linearly with a time schedule program, for mix. 2. Quantitation was achieved by UV detection at 220 nm. The second method was based on HPTLC separation followed by densitometric measurement of the spots at 254 nm. The separation was achieved on HPTLC silica gel F 254 plates using chloroform-ethanol-wateracetic acid (5:8:2:0.5, v/v/v/v) for mix. 1 and chloroform-ethanol-water-acetic acid (1.5:8:2:0.5, v/v/v/v) for mix. 2. The proposed methods were successfully applied for the analysis of pharmaceutical formulations containing the two multicomponent combinations.

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High-resolution/higher-order derivative spectrophotometry for determination of ternary mixtures of B-complex vitamins in pharmaceuticals

Cited by 28 publications

References 1 publication

Derivative and Derivative Ratio Spectrophotometric Analysis of Antihypertensive Ternary Mixture of Amiloride Hydrochloride, Hydrochlorothiazide and Timolol Maleate

Derivative and Derivative Ratio Spectrophotometric Analysis of Antihypertensive Ternary Mixture of Amiloride Hydrochloride, Hydrochlorothiazide and Timolol Maleate

Synthesis of a novel fluorescent probe based on 7‐nitrobenzo‐2‐oxa‐1,3‐diazole skeleton for the rapid determination of vitamin B₁₂ in pharmaceuticals

Application of LC and HPTLC-densitometry for the simultaneous determination of two multicomponent mixtures containing pyridoxine hydrochloride and cyanocobalamine

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High-resolution/higher-order derivative spectrophotometry for determination of ternary mixtures of B-complex vitamins in pharmaceuticals

Cited by 28 publications

References 1 publication

Derivative and Derivative Ratio Spectrophotometric Analysis of Antihypertensive Ternary Mixture of Amiloride Hydrochloride, Hydrochlorothiazide and Timolol Maleate

Derivative and Derivative Ratio Spectrophotometric Analysis of Antihypertensive Ternary Mixture of Amiloride Hydrochloride, Hydrochlorothiazide and Timolol Maleate

Synthesis of a novel fluorescent probe based on 7‐nitrobenzo‐2‐oxa‐1,3‐diazole skeleton for the rapid determination of vitamin B12 in pharmaceuticals

Application of LC and HPTLC-densitometry for the simultaneous determination of two multicomponent mixtures containing pyridoxine hydrochloride and cyanocobalamine

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Synthesis of a novel fluorescent probe based on 7‐nitrobenzo‐2‐oxa‐1,3‐diazole skeleton for the rapid determination of vitamin B₁₂ in pharmaceuticals