“…In addition, the most common requirement for the analysis of VB 12 is in the quality control of pharmaceuticals (tablets or injections), blood plasma serum, milk products for infants and fermentation products in which microorganisms can bond and release VB 12 (1). Presently, there are different methods used for measuring VB 12 concentration, including microbiological assay (2)(3)(4), spectrophotometry (5,6), chemiluminescence (7,8), atomic absorption spectrometry (9), capillary electrophoresis (10), high-performance liquid chromatography (11)(12)(13)(14)(15) and inductive-coupled plasma-mass spectrometry method (16). None of them, however, was very suitable in terms of simplicity, rapidity and accuracy.…”