High Resolution Fluoride Profiles of Artificial in vitro Lesions Treated with Fluoride Dentifrices and Mouthrinses during pH Cycling Conditions

Nelson, Douglas C.; Coote, G.E.; Shariati, Mohammad; Featherstone, J.D.B.

doi:10.1159/000261448

Cited by 16 publications

(8 citation statements)

References 9 publications

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“…pH-cycling models have also been employed to verify the performance of the association between fluoridated dentifrices and other measures for caries prevention such as CO 2 laser irradiation 81 , F varnishes 57 , F solutions 64 or caries treatment such as fluoride-releasing or not dental materials 22,36,77 (Figure 6). The association between fluoridated dentifrices and other caries-preventive measures, such as CO 2 laser irradiation 81 or fluoride varnishes was not able to increase the cariostatic action of the F dentifrices in pH-cycling protocols.…”

Section: Results and Literature Reviewmentioning

confidence: 99%

pH-cycling models for in vitro evaluation of the efficacy of fluoridated dentifrices for caries control: strengths and limitations

et al. 2010

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Despite a plethora of in situ studies and clinical trials evaluating the efficacy of fluoridated dentifrices on caries control, in vitro pH cycling models are still broadly used because they mimic the dynamics of mineral loss and gain involved in caries formation. This paper critically reviews the current literature on existing pH-cycling models for the in vitro evaluation of the efficacy of fluoridated dentifrices for caries control, focusing on their strengths and limitations. A search was undertaken in the MEDLINE electronic journal database using the keywords "pH-cycling", "demineralization", "remineralization", "in vitro", "fluoride", "dentifrice". The primary outcome was the decrease of demineralization or the increase of remineralization as measured by different methods (e.g.: transverse microradiography) or tooth fluoride uptake. Inclusion of studies, data extraction and quality assessment were undertaken independently and in duplicate by two members of the review team. Disagreements were solved by discussion and consensus or by a third party. One hundred and sixteen studies were included, of which 42 addressed specifically the comparison of dentifrices using different pH-cycling models. The other studies included meta-analysis or reviews, data about the effect of different fluoride sources on de-remineralization, different methods for analysis de-remineralization and chemical variables and characteristics of dental hard tissues that might have influence on de-remineralization processes. Generally, the studies presented ability to detect known results established by clinical trials, to demonstrate dose-related responses in the fluoride content of the dentifrices, and to provide repeatability and reproducibility between tests. In order to accomplish these features satisfactorily, it is mandatory to take into account the type of substrate and baseline artificial lesion, as well as the adequate response variables and statistical approaches to be used. This critical review of literature showed that the currently available pH-cycling models are appropriate to detect dose-response and pH-response of fluoride dentifrices, and to evaluate the impact of new active principles on the effect of fluoridated dentifrices, as well as their association with other anti-caries treatments.

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Section: Results and Literature Reviewmentioning

confidence: 99%

pH-cycling models for in vitro evaluation of the efficacy of fluoridated dentifrices for caries control: strengths and limitations

et al. 2010

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“…Many previous in vitro studies have investigated the mechanisms underlying lesion remineralization of enamel [Damato et al, 1990;Lammers et al, 1991] or in evaluating caries prevention products such as fluoride dentifrices [Nelson et al, 1992;Itthagarun and Wei, 2000]. To our knowledge, however, very few studies have focused on the influence of salivary proteins on enamel lesion remineralization, and limited information is available on the interaction between fluoride and salivary proteins [Margolis et al, 1982].…”

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confidence: 99%

Influence of Salivary Macromolecules and Fluoride on Enamel Lesion Remineralization in vitro

et al. 2007

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The aim of this study was to investigate the influence of salivary macromolecules on enamel lesion remineralization in the presence or absence of fluoride. Paraffin-stimulated whole saliva was centrifuged, and the supernatant was dialyzed in 1,000 molecular-weight cutoff dialysis tubes, first against a phosphate buffer and then against a mineral solution containing Ca and phosphate. Artificial subsurface lesions of human enamel, created in pH 4.5 acetate buffer, were remineralized for 28 days in 4 remineralizing solutions: group C – mineral solution as a control; group S – mineral solution + dialyzed saliva; group F – mineral solution + 1 ppm F; group SF – mineral solution + dialyzed saliva + 1 ppm F. Changes in relative mineral concentration in the lesions were assessed by transverse microradiography. The results showed statistically significant mineral gains in the lesion body in groups C (ΔZ = 3,254 ± 1,562% ×µm) and SF (ΔZ = 2,973 ± 1,349% ×µm), but not in groups S (ΔZ = 5,192 ± 1,863% ×µm) and F (ΔZ = 4,310 ± 1,138% ×µm) compared with the baseline group (ΔZ = 5,414 ± 461% ×µm). It was also found that the mineral density at the surface layer in group F (75.0 ± 15.7%) was greater than that in the baseline group (30.1 ± 12.3%) with statistical significance, but not in group SF (39.9 ± 16.5%). It was concluded that the macromolecules inhibited lesion remineralization fundamentally but that these molecules, in the presence of fluoride, seemed to play an important role in the continuation of remineralization by reducing mineral gains at the surface layer.

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“…These experiments were designed to simulate the dynamic variations in mineral saturation and pH associated with the natural carious process. There have since been many experiments to test the remineralising efficacy of various products on permanent teeth, utilising de/remineralising experiments 12,13,20,21 . However, there have been only a few studies on the de/remineralisation of carious lesions of enamel in primary teeth [22][23][24][25] .…”

mentioning

confidence: 99%

Effects of child formula dentifrices on artificial caries like lesions using in vitro pH-cycling: preliminary results

Itthagarun¹,

King²,

Rana³

2007

International Dental Journal

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High Resolution Fluoride Profiles of Artificial in vitro Lesions Treated with Fluoride Dentifrices and Mouthrinses during pH Cycling Conditions

Cited by 16 publications

References 9 publications

pH-cycling models for in vitro evaluation of the efficacy of fluoridated dentifrices for caries control: strengths and limitations

pH-cycling models for in vitro evaluation of the efficacy of fluoridated dentifrices for caries control: strengths and limitations

Influence of Salivary Macromolecules and Fluoride on Enamel Lesion Remineralization in vitro

Effects of child formula dentifrices on artificial caries like lesions using in vitro pH-cycling: preliminary results

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