High resolution field desorption mass spectrometry: II—glycosides

Hr, Schulten; De, Games

doi:10.1002/bms.1200010207

Cited by 68 publications

(7 citation statements)

References 23 publications

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“…In light of such chloride binding, field desorption mass spectra of hemes must be interpreted with caution. Counter ions have been observed previously with field desorption mass spectrometry [13].…”

Section: Compoundmentioning

confidence: 70%

High-pressure liquid chromatography and field desorption mass spectrometry of heme a, Heme a dimethyl ester and acetyl heme a dimethyl ester

DeFilippi¹,

Hultquist²

1977

Biochimica et Biophysica Acta (BBA) - General Subjects

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Field desorption mass spectrometry was shown to be a valid technique for determining the molecular weights of hemins and hemin esters, as well as of porphyrins. The observed base peaks of ligand-free protoheme IX, protoheme IX dimethyl ester, and protoporphyrin IX dimethyl ester correspond well to the molecular weights of these compounds and the base peak for hematoporphyrin corresponds to the molecular weight of this porphyrin minus two molecules of water. The technique was employed to confirm the molecular weights of heme a, heme a dimethyl ester, and acetyl heme a dimethyl ester. Heme a dimethyl ester was prepared by reaction of heine a with trimethyloxonium tetrafluoroborate and purified by high-pressure liquid chromatography. The isolated product was converted to the acetylated derivative by reaction with acetic anhydride and was subsequently purified by high-pressure liquid chromatography. A field desorption spectrum of heme a shows a base peak at 582 which is in agreement with previous deductions of the structure of this prosthetic group. Base peaks of the heme a ester and its acetylated derivative demonstrate that the two carboxyl groups have been methylated and the single hydroxyl group has been acetylated without further alteration of the molecule.

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Section: Compoundmentioning

confidence: 70%

High-pressure liquid chromatography and field desorption mass spectrometry of heme a, Heme a dimethyl ester and acetyl heme a dimethyl ester

DeFilippi¹,

Hultquist²

1977

Biochimica et Biophysica Acta (BBA) - General Subjects

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“…des terminalen Zuckers ermoglicht [3] und durch enzymatische Hydrolysemethoden als Isorhamnetin-3-O-rutinosid, Isorhamnetin-3-O-robinobiosid undals Gemische vorliegend -Quercetinbzw. Kämpferol-3-O-rutinosid und -robinobiosid identifiziert werden.…”

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Die Flavonoide in den Blüten von Primula officinalis

Karl¹,

Müller²,

Pedersen³

1981

Planta Med

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From methanolic extracts of fresh flowers of Primula officinalis 19 flavonoids were isolated or detected: quercetin, luteolin, kaempferol, isorhamnetin, apigenin; quercetin 3-O-glucoside, -rutinoside, -robinobioside, -gentiobioside, -(glucosyl-(1-->2) -glucosyl-(1-->6))-glucoside, -(rhamnosyl)-robinobioside; isorhamnetin 3-O-glucoside, -rutinoside, -robinobioside, -(rhamnosyl) -robinobioside; kaempferol 3-O-rutinoside, -robinobioside; limocitrin 3-O-glucoside; 3', 4'-dihydroxyflavonglucoside. Other compounds isolated were epicatechin, epigallocatechin, and proanthocyanidin B 2.

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“…Field desorption mass spectrometry (FD-MS) has shown great promise as a method of analysis for many types of nonvolatile chemical compounds (1)(2)(3)(4)(5)(6)(7)(8)(9)(10). In this technique, a sample is deposited on an emitter anode of activated tungsten wire and placed in a high electric field in the mass spectrometer.…”

mentioning

confidence: 99%

Emitter current programmer for field desorption mass spectrometry

Maine¹,

Soltmann²,

Holland³

et al. 1976

Anal. Chem.

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The practice of field desorptlon mass spectrometry has shown that continuous control of the emitter temperature is essential both for the production of quality FD spectra and for obtaining reproducible results from successlve analyses of the same sample. In this study, an attempt to obtain temperature regulation by means of emitter current control has been made. A solid state device has been developed that regulates the current through the emitter wire to within 0.1 mA at any level between 0 and 80 mA. The unit also provides linear programming from an initial to a final current at any of four selectable rates. The resulting current regulation has provided adequate temperature regulation to enable, with ease, the determination of the optimum anode temperature (current) for obtaining the best FD spectra for each of the various compounds analyzed. Reproducibility between different emitters is sufficient to allow separations of sorbed mixtures based on selection of successive optimum temperatures by pre-determined current adjustments. Repetitive scanning of the mass spectrometer under computer control during current programming results in the production of reconstructed mass chromatograms plotted, in essence, on a thermal axis. This device has been called an emitter current programmer (ECP) and in studies, to date, has been essential for the production of reproducible FD spectra, and when used in the programmed mode shows great promise as a new approach to resolving solid phase mixtures by differential desorption processes.Field desorption mass spectrometry (FD-MS) has shown great promise as a method of analysis for many types of nonvolatile chemical compounds (1-10). In this technique, a sample is deposited on an emitter anode of activated tungsten wire and placed in a high electric field in the mass spectrometer. Ions are produced by desorption of molecules from the solid phase matrix by a quantum mechanical tunneling effect (11). As an aid in the production of ions, thermal energy is applied to the sorbed sample by passing a small current through the emitter wire (12).Several experimental difficulties have been encountered by users of this technique (13,14). A major problem has been the generation of reproducible mass spectra from successive analyses of the sample. Experience has revealed that the standard method of manual control of the emitter current, by means of a ten-turn helipot, is ineffective in reproducing the emitter heating parameter. This shortcoming has been overcome with a solid state emitter current programmer (ECP), which provides a mechanism of precisely controlling the magnitude and rate of change of current in the emitter wire. The details of the fabrication of the ECP are provided in this note, along with preliminary studies that illustrate the impact this device can have on FD-MS. Of particular note are the capability of providing optimum temperature regulation for specific ion desorpPresent address, Institute for Basic Research in Mental Retardation, Staten Island, New York 10314.tion studies...

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High resolution field desorption mass spectrometry: II—glycosides

Cited by 68 publications

References 23 publications

High-pressure liquid chromatography and field desorption mass spectrometry of heme a, Heme a dimethyl ester and acetyl heme a dimethyl ester

High-pressure liquid chromatography and field desorption mass spectrometry of heme a, Heme a dimethyl ester and acetyl heme a dimethyl ester

Die Flavonoide in den Blüten von Primula officinalis

Emitter current programmer for field desorption mass spectrometry

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