High‐resolution Far‐field Microscopy

Abstract

The developments in high‐resolution microscopy play a challenging role in understanding the fundamental processes in molecular and cell‐biology as well as material research. The most desired feature of any optical microscopy technique is the ability to perform in‐depth scanning of specimens and to distinguish extremely small organelles and their dynamics in the nanometer range. To answer a large variety of questions in all areas of biological and material research, it has become imperative to improve the spatial and temporal resolution of optical microscopes. However since the nineteenth century when Ernst Abbe first established the limit of an optical system to resolve two objects – the Abbe diffraction limit – the scientists have been struggling to approach nanometer resolution by defying the diffraction limit. These continued efforts have led to the development of novel super‐resolution technologies in microscopy that are discussed in this review.

Linear and Non-Linear Optical Imaging of Cancer Cells with Silicon Nanoparticles

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