High-resolution combinatorial 3D printing of gelatin-based biomimetic triple-layered conduits for nerve tissue engineering

Liu, Suihong; Sun, Liguo; Zhang, Haiguang; Hu, Qingxi; Wang, Yahao; Ramalingam, Murugan

doi:10.1016/j.ijbiomac.2020.11.010

Cited by 33 publications

(24 citation statements)

References 39 publications

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“…Furthermore, the hydrogel nature of gelatin film provides a porous sponge‐like architecture, which restrains cell diffusion and affords abundant nutrients leading to higher infiltration of cartilage cells. [ 34,46 ]…”

Section: Resultsmentioning

confidence: 99%

“…A triple‐layered conduits for nerve tissue engineering was fabricated using combinatorial approach comprising of E‐Jet printing, dip‐coating, and electrospinning. [ 34 ] Tissueengineering scaffolds fabricate with nanofibrous was fabricated by combining electrospinning and melt deposition. [ 35 ] A polycaprolactone (PCL)/alginate composite scaffolds was obtained using a combination method of wet electrospinning, rapid prototyping, and a physical punching process.…”

Section: Introductionmentioning

confidence: 99%

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Sandwich‐Like Gelatin/Polycaprolactone/Polyvinyl Pyrrolidone 3D Model with Significantly Improved Cartilage Cells Adhesion and Regeneration

Liu

et al. 2021

Macro Materials & Eng

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A novel sandwich‐like composite 3D model integrated with deposited gelatin film and printed polycaprolactone (PCL)/polyvinyl pyrrolidone (PVP) scaffold is proposed for 3D cartilage cell culture. The 3D model includes three layers, top scaffold, middle gelatin film, and basal slide. The printed scaffold layer mimicking extracellular matrix is employed to provide 3D regeneration architecture; gelatin film layer is used for fixing printed scaffold and catching falling cells. Electrospray and electrohydrodynamic jet printing technologies are combined to construct sandwich‐like composite 3D model. The blended constructing processes are investigated thoroughly both theoretically and experimentally. The retained charges on the gelatin film are influenced the electric field distribution and jet behaviors during printing. The characteristics of gelatin film and composite scaffold are studied. The thickness, surface roughness, and planeness of one layer of deposited gelatin film are 400 nm, 22 nm, and 2.27 µm; the size of printed PCL/PVP composite fiber is 10 µm. The fabricated sandwich‐like composite 3D model is proved to facilitate adhesion and ingrowth of cartilage cells.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sandwich‐Like Gelatin/Polycaprolactone/Polyvinyl Pyrrolidone 3D Model with Significantly Improved Cartilage Cells Adhesion and Regeneration

Liu

et al. 2021

Macro Materials & Eng

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“…Newer approaches have focused on customizing nerve guide conduits, such as using 3D printing, and creating synthetic scaffolds infused with neurotrophic factors and lined with Schwann cells to create a favorable microenvironment and facilitate axonal migration along these artificial channels, which may increase the likelihood of successful nerve regeneration [39,40,41 ▪ ,42 ▪▪ ]. Simultaneous fat grafting can provide adipose-derived stem cells, which can differentiate into Schwann-like cells that guide axonal regeneration, protect against scarring and provide a favorable microenvironment with pro-regenerative properties [43]. Other approaches such as using magnesium-based biomaterials and other nanoparticles to promote peripheral nerve regeneration are also under investigation [44 ▪ ,45].…”

Section: Managementmentioning

confidence: 99%

Traumatic peripheral nerve injuries: diagnosis and management

Barnes

Miller²,

Simon

2022

Current Opinion in Neurology

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Purpose of reviewTo review advances in the diagnostic evaluation and management of traumatic peripheral nerve injuries.Recent findingsSerial multimodal assessment of peripheral nerve injuries facilitates assessment of spontaneous axonal regeneration and selection of appropriate patients for early surgical intervention. Novel surgical and rehabilitative approaches have been developed to complement established strategies, particularly in the area of nerve grafting, targeted rehabilitation strategies and interventions to promote nerve regeneration. However, several management challenges remain, including incomplete reinnervation, traumatic neuroma development, maladaptive central remodeling and management of fatigue, which compromise functional recovery.SummaryInnovative approaches to the assessment and treatment of peripheral nerve injuries hold promise in improving the degree of functional recovery; however, this remains a complex and evolving area.

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“…In this regard, it is also worth mentioning that Suihong et al. [ 92 ] used EHD jet printing as one of the processes for the development of gelatin‐based biomimetic triple‐layered conduits for nerve tissue engineering. The EHD jet printing was applied to produce the innermost layer using PCL, which comprises the most intricate structural details, and the outer layer of PCL.…”

Section: Spinneret Configurationsmentioning

confidence: 99%

Management of Operational Parameters and Novel Spinneret Configurations for the Electrohydrodynamic Processing of Functional Polymers

Silva

Torres‐Giner

Vicente

et al. 2022

Macro Materials & Eng

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Functional materials have become key drivers in the development of multiple high‐end technologies. Electrohydrodynamic processing (EHDP) is a straightforward method to generate polymer micro‐ and nanostructures that can be applied to the food, pharmaceutical, environmental, and biomedical areas, among others, since these can yield materials with higher performance. Some of the EHDP's advantages over other polymer processing technologies rely on its high versatility, by which the final assembly can be modified in different ways to combine materials with multiple properties and also in different morphological structures, and the use of room processing conditions, meaning that thermolabile ingredients can be incorporated with minimal activity loss. This review provides the historical background, process basics, and the state‐of‐the‐art of the most recent advances achieved in the EHDP technology dealing with the control of its operational parameters to optimize processability and achieve end‐product quality and homogeneity. It also focuses on the newly developed modes of operation and spinneret configurations that can lead to the formation of a wide range of micro‐ and nanostructures with different functionalities and solve some of its current technical limitations. Finally, it also further highlights the potential applications of the resultant hierarchical functional polymer‐based materials obtained by these novel EHDP methods.

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High-resolution combinatorial 3D printing of gelatin-based biomimetic triple-layered conduits for nerve tissue engineering

Cited by 33 publications

References 39 publications

Sandwich‐Like Gelatin/Polycaprolactone/Polyvinyl Pyrrolidone 3D Model with Significantly Improved Cartilage Cells Adhesion and Regeneration

Sandwich‐Like Gelatin/Polycaprolactone/Polyvinyl Pyrrolidone 3D Model with Significantly Improved Cartilage Cells Adhesion and Regeneration

Traumatic peripheral nerve injuries: diagnosis and management

Management of Operational Parameters and Novel Spinneret Configurations for the Electrohydrodynamic Processing of Functional Polymers

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