High Rabdosiin and Rosmarinic Acid Production inEritrichium sericeumCallus Cultures and the Effect of the Calli on Masugi-Nephritis in Rats

Rosmarinic acid and its derivatives: biotechnology and applications

“…To change the rabdosiin/RA ratio to increase rabdosiin accumulation, elicitation of E. sericeum calli (E-4 line) by cuprum ions was performed (Inyushkina et al, 2007). The total content of polyphenols in the Cu +2 -glycerateelicited calli was similar to that in the non-elicited calli, but the rabdosiin proportion increased substantially, up to 4.1% of dry weight.…”

“…Skin protection against atopic dermatitis Jang et al, 2011 Protection of neurons against insults a Rosmarinic acid is a promising neuroprotective compound of potential use at the nutritional/ pharmaceutical interface Treatment of retinopathy Kim et al, 2009 Inhibition of TNF-α-induced ROS generation and NF-κB activation and activation of TNF-α-induced apoptosis a Ito et al, 1998 Hydroxyl radical scavenging (activity: rabdosiin > RA = Therapeutic agent for treatment of allergy ascorbic acid > caffeic acid) a Dose-dependent inhibition of excessive hyaluronidase activity (rabdosiin > RA > caffeic acid) a Inhibition of hexosaminidase release from RBL-2H3 cells (rabdosiin = caffeic acid > RA) a Anti-HIV activity a Inhibition of human immunodeficiency virus Kashiwada et al, 1995 Inhibition of HIV-1 integrase and DNA topoisomerase (activity: rabdosiin > RA) a Tewtrakul et al, 2003;Abd-Elazem et al, 2002 Anti-glomerulonephritic activity in rats with induced Masugi-nephritis (in combination with RA) b Treatment of acute forms of renal pathology Inyushkina et al, 2007;Bryukhanov et al, 2008 Lithospermic acid B (salvianolic acid B) Alleviation of symptoms connected with renal pathology a,b,c Treatment of diabetic nephropathy and microalbuminuria Li et al, 2001;Kang et al, 2008;Luo et al, 2008;Yao et al, 2009 Cardioprotective effect and improvement of myocardial microcirculation b Promising for treatment of diseases connected with microcirculatory injuries He et al, 2008 Protection of cerebral microvascular endothelial cells against H 2 O 2 -induced apoptosis a Liu et al, 2007 Anti-atherosclerotic effect b Hur et al, 2008 Protection of human endothelial cells from oxidative stress-induced damage a Wu et al, 2009 Anticancer activity associated with the prevention and treatment of head and neck squamous-cell carcinoma a,b (Paluszczak et al, 2010). RA was found to inhibit the migration of MDA-MB-231BO human bone-homing breast cancer cells and inhibited bone metastasis from breast carcinomas, mainly via the receptor activator of NF-κB ligand (RANKL)/RANK/osteoprotegerin pathway, and by simultaneously suppressing the expression of interleukin-8 .…”

“…Oral administration of E. sericeum callus biomass to rats with induced Masugi-nephritis (a rat model of experimental glomerulonephritis closely resembling human lobular glomerulonephritis) alleviated, and in some cases prevented the induced acute form of renal pathology (Inyushkina et al, 2007;Bryukhanov et al, 2008).…”

“…In suspension cultures of C. blumei, RA accumulated up to a content of 19% of the cell dry weight when the cells were cultured in a medium with high sucrose concentration (4%) (Petersen, 1991). However, an increase of sucrose concentration in the nutrient medium of 4-5% was inefficient for the E. sericeum E-4 callus culture (Inyushkina et al, 2007). Elicitation of RA biosynthesis by fungal elicitor preparations or methyl jasmonate was useful for the improvement of RAD yield in Orthosiphon aristatus (Sumaryono et al, 1991), L. erythrorhizon (Mizukami et al, 1992(Mizukami et al, , 1993, C. blumei (Szabo et al, 1999), O. basilicum (Bais et al, 2002), S. miltiorrhiza (Yan et al, 2006) and E. sericeum (Inyushkina et al, 2007) cell and root cultures.…”

“…However, an increase of sucrose concentration in the nutrient medium of 4-5% was inefficient for the E. sericeum E-4 callus culture (Inyushkina et al, 2007). Elicitation of RA biosynthesis by fungal elicitor preparations or methyl jasmonate was useful for the improvement of RAD yield in Orthosiphon aristatus (Sumaryono et al, 1991), L. erythrorhizon (Mizukami et al, 1992(Mizukami et al, , 1993, C. blumei (Szabo et al, 1999), O. basilicum (Bais et al, 2002), S. miltiorrhiza (Yan et al, 2006) and E. sericeum (Inyushkina et al, 2007) cell and root cultures. Methyl jasmonate enhanced both RA and lithospermic acid B accumulation in S. miltiorrhiza hairy root cultures, from 3.3% to 6%, and from 2.9% to 19.3% of dry weight, respectively (Xiao et al, 2009).…”