High production of succinyl isoflavone glycosides by Bacillus licheniformis ZSP01 resting cells in aqueous miscible organic medium

Abstract

To achieve efficient production of succinyldaidzin and succinylgenistin, resting cells of a solvent-stable strain Bacillus licheniformis ZSP01 were used to react with pure isoflavones or soybean flour extract in a reaction medium with 10% dimethyl sulfoxide. Strikingly, 0.8 mM daidzein, 0.8 mM genistein, 2.0 mM daidzin, and 2.0 mM genistin were transformed to succinyl isoflavone glycosides in 27 H (yield >90%). The soybean flour extract (6.1%, w/v) contained 0.32 mM daidzein, 0.84 mM daidzin, 0.38 mM genistein, and 1.04 mM genistin. Over 95% of total isoflavones (daidzein, daidzin, genistein, and genistin) in the soybean flour extract were converted to succinyl isoflavone glycosides after 27 H. Strain ZSP01 shows both high glycosylation and succinylation activities. These results suggest that B. licheniformis ZSP01 could be useful for the efficient production of succinyl soybean isoflavone glycosides.

Hypoglycemic Activity and the Potential Mechanism of the Flavonoid Rich Extract from Sophora tonkinensis Gagnep. in KK-Ay Mice

“…The two major principles were determined as maackiain ( 6 ; Lee et al, 2015) and sophoranone ( 10 ; Li et al, 2008). The remaining compounds ( Figure 1C ) were identified as genistin ( 1 ; Al-Maharik and Botting, 2008), trifolirhizin ( 2 ; Yang X.Z. et al, 2014), ononin ( 3 ; Yang X.Z.…”

“…L6 Cells were maintained in MEM-α and used for experiment described previously (Al-Maharik and Botting, 2008; Li et al, 2008; Lee et al, 2015). A cell-based 2-[ N -(7-nitrobenz-2-oxa-1,3-diaxol-4-yl)amino]-2-deoxyglucose (2-NBDG) Glucose Uptake Assay Kit (Cayman Chemical, USA) was used to measure the effects of ST-EtOAc in promoting the glucose uptake on L6 cells.…”

Changes in Isoflavone Profile from Soybean Seeds during Cheonggukjang Fermentation Based on High-Resolution UPLC-DAD-QToF/MS: New Succinylated and Phosphorylated Conjugates

“…Among them, 6 -O-succinyldaidzin of Japanese natto elicited anti-ischemic effects by reducing both infarct areas and neurological scores of the middle cerebral artery occlusion rats [20]. The Korean cheonggukjang presented significantly clinical effects in hyperlipidemia-induced hamsters [21], and 6 -O-succinyldaidzin and 6 -O-succinylgenistin derived from these foods were rapidly accumulated after 15-18 h prior to maximum conversion after 27-36 h during their fermentation [22,23].…”

Mining of efficient microbial UDP-glycosyltransferases by motif evolution cross plant kingdom for application in biosynthesis of salidroside

“…We have previously isolated bacterium Bacillus licheniformis ZSP01 on the basis of its ability to efficiently glycosylate coumarin and isoflavone, which are important secondary metabolites in the plant 20,21 . In the present study, we analyzed GTs from typical strain Bacillus licheniformis 9945A and many GT genes belong to several main GT families from various plants and some microorganisms, by bioinformatics approaches.…”