High Prevalence of <i>Helicobacter pylori</i> Detected by PCR in the Oral Cavities of Periodontitis Patients

Umeda, Makoto; Kobayashi, Hiroaki; Takeuchi, Yasuo; Hayashi, Joichiro; Morotome-Hayashi, Yoko; Yano, Kazuko; Aoki, Akira; Ohkusa, Toshifumi; Ishikawa, Isao

doi:10.1902/jop.2003.74.1.129

Cited by 106 publications

(125 citation statements)

References 27 publications

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“…Some epidemiological studies have suggested the presence of H. pylori in contaminated water supplies (14), and H. pylori DNA has been amplified from well water, saliva, and feces (2,3,10,25), yet the bacterium has rarely been cultured from these sources (5). F-12 with serum is superior to MuellerHinton broth with serum for the recovery of H. pylori from frozen stocks (23).…”

Section: Discussionmentioning

confidence: 99%

Nutritional Requirements and Antibiotic Resistance Patterns ofHelicobacterSpecies in Chemically Defined Media

et al. 2006

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The growth of the gastric pathogen Helicobacter pylori in the absence of serum remains challenging, and nutritional requirements have only partially been defined, while almost nothing is known about nutritional requirements of other Helicobacter spp. Although previous data showed that H. pylori grows in the chemically defined medium F-12, but not in other tissue culture media examined, the specific components responsible for growth were not entirely understood. Here we describe the optimization of amino acids, metals, and sodium chloride for H. pylori. Iron, zinc, and magnesium were critical for growth; copper was not required. Optimization of sodium chloride was further beneficial. Nutritional requirements and antibiotic resistance patterns of several other Helicobacter spp. revealed that all except H. felis grew in serum-free, unsupplemented F-12. All Helicobacter spp. were resistant to at least six antimicrobial agents when cultured in the presence of serum. However, in the absence of serum, H. pylori, H. mustelae, and H. muridarum became sensitive to polymyxin B and/or trimethoprim. Much of the data were obtained using a convenient ATP assay to quantify growth. H. pylori has surprisingly few absolute requirements for growth: 9 amino acids, sodium and potassium chloride, thiamine, iron, zinc, magnesium, hypoxanthine, and pyruvate. These data suggest that H. pylori and other Helicobacter spp. are not as fastidious as previously thought. The data also suggest that chemically defined media described herein could yield the growth of a wide range of Helicobacter spp., allowing a more detailed characterization of Helicobacter physiology and interactions with host cells.

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Section: Discussionmentioning

confidence: 99%

Nutritional Requirements and Antibiotic Resistance Patterns ofHelicobacterSpecies in Chemically Defined Media

et al. 2006

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“…Our study found conflicting results when H pylori infection status in three comparison controls was compared to that in oral cancer patients. Previous studies have shown that H pylori was detectable in the oral cavity by histopathologic diagnosis or polymerase chain reaction [39,40] , but this bacterium may be present as a transient organism because of the difficulty of surviving in the oral cavity [41] . Thus, further studies are necessary to elucidate the relationship between H pylori infection and oral cancer risk.…”

Section: Discussionmentioning

confidence: 99%

Association between Helicobacter pylori seropositivity anddigestive tract cancers

Wu²,

Yu³

et al. 2009

WJG

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AIM:To explore the role of Helicobacter pylori (H pylori ) infection on the risk of digestive tract cancers. METHODS:In total, 199 oral squamous-cell carcinoma (SCC), 317 esophageal SCC, 196 gastric cardia and non-cardia adenocarcinoma and 240 colon adenocarcinoma patients were recruited for serum tests of H pylori infection. Two hospital-and one community-based control groups were used for the comparisons. H pylori seropositivity was determined by an enzyme linked immunosorbent assay method against H pylori IgG. RESULTS: Presence of H pylori infection was signifi-cantly inversely associated with esophageal SCC [adjusted odds ratio (AOR): 0.315-0.472, all P -value < 0.05] but positively associated with gastric adenocarcinoma (both cardia and non-cardia) (AOR: 1.636-3.060, all P -value < 0.05) in comparison to the three control groups. Similar results were not found in cancers of the oral cavity and colon. CONCLUSION: Our findings support the finding thatH pylori seropositivity is inversely associated with esophageal SCC risk, but increases the risk of gastric cardia adenocarcinoma.

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“…29,30 Dental plaque and/or periodontal inflammation may influence pathogenic processes occurring in distally contiguous mucosal surfaces, for example, in the respiratory or digestive tracts. 31,32 Salivary hydrolytic enzymes, observed to be elevated in patients with periodontitis, can promote the adhesion of pathogenic bacteria to the oral surfaces, thereby altering oropharyngeal colonization patterns. It is also possible that periodontopathic bacteria stimulate the periodontium to release proinflammatory cytokines that, when aspirated or swallowed, alter mucosal surfaces to promote adhesion of pathogenic bacteria that cause diseases such as pneumonia or gastric ulcers.…”

Section: Gingival Inflammation: Pathways Of Systemic Effectsmentioning

confidence: 99%

“…It is also possible that periodontopathic bacteria stimulate the periodontium to release proinflammatory cytokines that, when aspirated or swallowed, alter mucosal surfaces to promote adhesion of pathogenic bacteria that cause diseases such as pneumonia or gastric ulcers. 31,32 Finally, cytokines released from inflamed periodontal tissues may enter the respiratory tract in aspirated saliva, triggering the sequence of neutrophil recruitment, epithelial damage, and infection. 31 …”

Section: Gingival Inflammation: Pathways Of Systemic Effectsmentioning

confidence: 99%

Periodontal Inflammation: From Gingivitis to Systemic Disease?

Scannapieco

2011

Gingival Diseases - Their Aetiology, Prevention and Treatment

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High Prevalence of Helicobacter pylori Detected by PCR in the Oral Cavities of Periodontitis Patients

Abstract: Close attention should be given to periodontitis patients who harbor H. pylori in the oral cavity.

Cited by 106 publications

References 27 publications

Nutritional Requirements and Antibiotic Resistance Patterns ofHelicobacterSpecies in Chemically Defined Media

Nutritional Requirements and Antibiotic Resistance Patterns ofHelicobacterSpecies in Chemically Defined Media

Association between Helicobacter pylori seropositivity anddigestive tract cancers

Periodontal Inflammation: From Gingivitis to Systemic Disease?

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