High prevalence of blaCTX-M extended-spectrum β-lactamase genes in Escherichia coli isolates from pets and emergence of CTX-M-64 in China

Sun, Yanwu; Zeng, Zhenling; Chen, Sheng; Ma, Junying; He, Liang-Ying; Liu, Y.; Deng, Yuting; Lei, Tao; Zhao, Jinfeng; Liu, J.-H.

doi:10.1111/j.1469-0691.2010.03127.x

Cited by 151 publications

(133 citation statements)

References 27 publications

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“…bla CTX-M+TEM was the dominant bla gene type, which is consistent with the results of other studies detecting these genes in ESBL producers from companion animals in China and other counties [11,16,31]. CTX-M-15-producing E. coli of ST131 was not found in the present study, which is regarded as an emerging human pandemic clone [32].…”

Section: Discussionsupporting

confidence: 82%

Characterization of ESBL-producing Escherichia coli recovered from companion dogs in Tai'an, China

Liu

Zhou³

et al. 2017

J Infect Dev Ctries

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Introduction: Animals are considered to be reservoirs of extended-spectrum beta-lactamase (ESBL)-producing bacteria, but few epidemiological data on ESBL-producing Escherichia coli urinary tract isolates in pet dogs are available in China. Methodology: This study was conducted to describe the prevalence and characterization of ESBL producers among E. coli urinary tract isolates from pet dogs in Tai'an, China. Results: A total of 118 E. coli were obtained from urinary samples of 80 companion dogs suffering from acute or chronic cystitis, of which three isolates from different dogs were ESBL producers. One isolate from dog A was of phylogroup A/ST410/CTX-M-15/TEM-1; one from dog B was of phylogroup B1/ST533/CTX-M-15/TEM-1; one from dog C was of phylogroup D/ST648/CTX-M-15. All ESBL producers were resistant to ampicillin, cephalexin, cefalotin, cefpodoxime, ceftiofur, enrofloxacin, marbofloxacin, and trimethoprim/sulfamethoxazole, but were susceptible to imipenem and amoxicillin/clavulanic acid. E. coli of ST533 carrying blaCTX-M-15 were first detected in pet dogs in China. Conclusions: Collectively, the findings could expand our knowledge about the prevalence and characterization of ESBL-producing E. coli urinary tract isolates in pet dogs in China.

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Section: Discussionsupporting

confidence: 82%

Characterization of ESBL-producing Escherichia coli recovered from companion dogs in Tai'an, China

Liu

Zhou³

et al. 2017

J Infect Dev Ctries

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“…The recipient strain was sensitive to cefazolin, cephalexin, ceftriaxone and cefotaxime and was not carrying bla CTX-M-1 and/or bla TEM genes in its plasmid as confirmed by PCR analysis. In vitro mating experiments were performed by broth mating [19], filter paper mating [20] and plate mating [21]. Transconjugants were selected on Mac Conkey's agar containing ceftriaxone (50 lg/ml) and nalidixic acid (100 lg/ml).…”

Section: Horizontal Gene Transfermentioning

confidence: 99%

PCR-Based Detection of Extended-Spectrum β-Lactamases (bla CTX-M-1 and bla TEM ) in Escherichia coli, Salmonella spp. and Klebsiella pneumoniae Isolated from Pigs in North Eastern India (Mizoram)

et al. 2013

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Cephalosporins are major antimicrobials used to treat serious infections. However, their effectiveness is being compromised by the emergence of extendedspectrum b-lactamases (ESBLs). A total of 138 enteric bacteria were isolated from 53 faecal samples of pigs collected from different districts of Mizoram, of which 102 (73.91 %) were Escherichia coli, 26 (18.84 %) were Salmonella spp. and 10 (7.25 %) were Klebsiella pneumoniae. Phenotypic confirmatory test (Double Discs Synergy Test) showed that 8 (5.80 %) E. coli isolates were ESBLs producer. PCR analysis confirmed that out of the eight isolate, 7 (5.07 %) harboured bla CTX-M-1 gene and/or bla TEM gene. Of the eight positive isolates, 7 (5.07 %) and 3 (2.17 %) were found to be positive for bla CTX-M-1 gene and bla TEM gene, respectively, of which 3 (2.17 %) isolates were positive for both the genes. Only 4 (2.90 %) E. coli isolates carried bla CTX-M-1 gene alone. Agarose gel electrophoresis showed that all the isolates were carrying plasmids ranging between 0.9 and *30 kb. Out of the seven isolates positive for bla CTX-M-1 and/or bla TEM , 2 (1.84 %) isolates were confirmed for bla CTX-M-1 gene in their plasmid. Only one E. coli isolate was found to be positive for both the genes in its plasmid. The resistance plasmid could not be transferred to a recipient by in vitro horizontal gene transfer method.

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“…CTX-M-55 first isolated from E. coli in Thailand [33] developed from one point mutation of CTX-M-15, leading to replacement of amino acid 80.In recent years, CTX-M-55 in E. coli was separated from food animals and pets in China [33][34][35][36][37], but was very rare in clinical isolates. Yu et al [10] first detected it in S. Typhimurium from the stool of a child in 2011.…”

Section: Discussionmentioning

confidence: 99%

Prevalence, resistance, and extended-spectrum beta-lactamases among Salmonella from patients in northeast China

Zhang¹,

Wang²,

Li³

et al. 2017

J Infect Dev Ctries

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Introduction: Salmonella is an important infection in Liaoning Province. Serotype and resistance levels are poorly studied. Knowledge of cephalosporin-resistant genes is needed to provide information to support the clinical diagnosis and to guide therapy. Methodology: A total of 172 isolates were collected from January 2009 to March 2014. Resistance to cephalosporin, fluoroquinolones, and other conventional first-line antibiotics was detected using the disk diffusion and agar dilution methods. The relatedness of isolates with the same serotype was analyzed by pulsed-field gel electrophoresis (PFGE). Genes encoding extended-spectrum β-lactamase enzymes (blaCTX-M, blaTEM, blaSHV, and blaOXA) were identified by polymerase chain reaction (PCR) and sequencing in cephalosporin-resistant strains. Results: S. Enteritidis, 1,4,[5],12:i:-, and S .Typhimurium were the most common serotypes. The resistance rate of Salmonella to conventional first-line antibiotics sulfamethoxazole-trimethoprim, tetracycline, chloramphenicol, and ampicillin was 21%,33%,37%, and 55%,respectively. To fluoroquinolones (gatixacin, levofloxacin, and ciprofloxacin), the resistance rates were 7%,18%, and 23%, respectively, whereas for extended-spectrum cephalosporins(ceftazidime, cefepime, and ceftriaxone), there sistance rates were 4%,8%, and 9%. In total, 14blaCTX-M, 14 blaTEM-1, and 1blaOXA-1 were found in 15 Salmonella isolates resistant to cephalosporin. No blaSHVwas detected. The blaCTX-M-55 gene was the most frequently encountered gene. The blaCTX-M-65 gene from S. Enteritidis is described for first time in Salmonella from humans. Results of PFGE indicated strong similarity among Salmonella isolates in Liaoning Province. Conclusions: Extended-spectrum cephalosporins are the first choice for the treatment of infection with Salmonella, but blaCTX-M-encoded resistance, including blaCTX-M-65, is present.

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High prevalence of blaCTX-M extended-spectrum β-lactamase genes in Escherichia coli isolates from pets and emergence of CTX-M-64 in China

Cited by 151 publications

References 27 publications

Characterization of ESBL-producing Escherichia coli recovered from companion dogs in Tai'an, China

Characterization of ESBL-producing Escherichia coli recovered from companion dogs in Tai'an, China

PCR-Based Detection of Extended-Spectrum β-Lactamases (bla CTX-M-1 and bla TEM ) in Escherichia coli, Salmonella spp. and Klebsiella pneumoniae Isolated from Pigs in North Eastern India (Mizoram)

Prevalence, resistance, and extended-spectrum beta-lactamases among Salmonella from patients in northeast China

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