High Prevalence and Properties of Enterotoxin-Producing<i>Staphylococcus aureus</i>ST5 Strains of Food Sources in China

Chang, Yanzi; Gao, Hong; Zhu, Zhifeng; Ye, Shuo; Yang, Yung‐Hun; Shen, Xuanyi; Zhang, Danyang; Song, Qifan

doi:10.1089/fpd.2015.2085

Cited by 7 publications

(5 citation statements)

References 25 publications

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“…Nevertheless, ( Veras et al, 2008 ) found that each of the SEs ( SEB , SEC or SED ) alone or in combination with other genes were concerned in food poisoning eruptions all over the world. On the other hand, the results of ( Gholamzad et al, 2015 , Chang et al, 2016 ) disagreed with our findings; these studies found that the SEB gene was the most common and prevalent S. aureus enterotoxin gene.…”

Section: Discussioncontrasting

confidence: 99%

Antibiogram and phylogenetic diversity of enterotoxigenic Staphylococcus aureus strains from milk products and public health implications

Abdeen

Mousa

Salam³

et al. 2020

Saudi Journal of Biological Sciences

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Food poisoning caused by Staphylococcus aureus ( S. aureus ) toxins is considered one of the foremost public health threat that usually occurs through the ingestion of raw milk contaminated with staphylococcal enterotoxins. The current study spotlights on the prevalence, antibiogram and genetic diversity of S. aureus enterotoxin genes. One hundred and fifty of raw milk (90) and ice cream (60) samples were randomly collected from local markets from Sadat city, Egypt . S. aureus was recovered from 44% of raw milk and 20% of ice cream samples. The identification for the obtained S. aureus isolates was confirmed through targeting the nuc gene. Antibiogram pattern of 32 S. aureus isolates showed high resistance to Cefoxitin, Sulpha/Trimethoprim, Tetracycline, Norfloxacin, Penicillin and Cephradine. However, high susceptibility to Gentamycin and Vancomycin were observed. Multiplex PCR was a competent practise for the recognition of Staphylococcus enterotoxin (SE) genes ( SEA , SEB and SED ). The phylogenetic analysis of the SED gene of enterotoxigenic S. aureus strains showed identical similarity with 100% to each other and high similarity with other international isolates in GenBank from different localities and sources. The frequency of enterotoxigenic S. aureus strains in milk products could have serious hazardous effects on humans. These results suggested possible strains transmission between different geographical areas through the food and milk product trades.

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Section: Discussioncontrasting

confidence: 99%

Antibiogram and phylogenetic diversity of enterotoxigenic Staphylococcus aureus strains from milk products and public health implications

Abdeen

Mousa

Salam³

et al. 2020

Saudi Journal of Biological Sciences

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“…Linking ST to toxin gene presence, only ST5 and ST30 strains harbored SE-encoding genes in this study. In fact, assessing the MLST profile to the toxigenic potential of S. aureus food sources in China ( Chang et al, 2016 ) has illustrated the specific ability of ST5 strains to harbor and to produce SE while the other ST, including ST1, ST188, ST97 and ST398, did not, as we also noticed.…”

Section: Discussionsupporting

confidence: 59%

Staphylococcus aureus in Some Brazilian Dairy Industries: Changes of Contamination and Diversity

et al. 2017

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Staphylococcus aureus, a major food-poisoning pathogen, is a common contaminant in dairy industries worldwide, including in Brazil. We determined the occurrence of S. aureus in five dairies in Brazil over 8 months. Of 421 samples, 31 (7.4%) were positive for S. aureus and prevalence varied from 0 to 63.3% between dairies. Sixty-six isolates from the 31 samples were typed by Multi-Locus Sequence Typing to determine if these isolates were persistent or continuously reintroduced. Seven known sequence types (STs), ST1, ST5, ST30, ST97, ST126, ST188 and ST398, and four new ST were identified, ST3531, ST3540, ST3562 and ST3534. Clonal complex (CC) 1 (including the four new ST), known as an epidemic clone, was the dominant CC. However, there were no indications of persistence of particular ST. The resistance toward 11 antibiotic compounds was assessed. Twelve profiles were generated with 75.8% of strains being sensitive to all antibiotic classes and no Methicillin-resistant S. aureus (MRSA) strains were found. The enterotoxin-encoding genes involved in food-poisoning, e.g., sea, sed, see, and seg were targeted by PCR. The two toxin-encoding genes, sed and see, were not detected. Only three strains (4.5%) harbored seg and two of these also harbored sea. Despite the isolates being Methicillin-sensitive S. aureus (MSSA), the presence of CC1 clones in the processing environment, including some harboring enterotoxin encoding genes, is of concern and hygiene must have high priority to reduce contamination.

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“…Soriano et al [55] reported that seb and sed were the predominant SEs in food handlers, while Collery et al [56] reported that seb is the SE most shared among nasal isolates. Chang et al and Gholamzad et al [57,58] disagreed with our findings in that they reported that seb was the most common S. aureus enterotoxin gene.…”

Section: Resultscontrasting

confidence: 92%

Molecular detection of Staphylococcus aureus enterotoxin genes isolated from mastitic milk and humans in El-Behira, Egypt

et al. 2021

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Background and Aim: Milk is a chief source of many nutrients. However, we must also bear in mind that it is a potential source for many cases of food poisoning. This study was conducted to investigate the prevalence of cow mastitis and evaluate the presence of enterotoxins and antibiotic resistance patterns in Staphylococcus aureus isolated from milk and contact humans in El-Behira Province, Egypt. Materials and Methods: A total of 680 milk samples from 170 cows and 86 human samples consisting of 43 hand swabs and 43 nasal swabs were analyzed. The milk samples were subjected to the California mastitis test. Results: The general occurrence was 23.1% (157/680) where 48 quarters had clinical mastitis and 109 had subclinical mastitis. Subsequently, S. aureus was isolated in Baird-Parker agar where typical and atypical colonies were selected and submitted to coagulase and complementary tests. Out of 48 samples of mastitic milk studied, 16 (33.3%) showed contamination by S. aureus whereas 109 samples of subclinical mastitis showed contamination in only 18 (16.5%). On the opposite hand, of the 86 human samples, 33 revealed S. aureus contamination, corresponding to 38.37% of the samples. Furthermore, multiplex polymerase chain reaction targeting nuc and the staphylococcal enterotoxin-encoding genes sea, seb, sec, sed, and see were performed after culture, revealing that 88.2% (30/34) of milk samples and 93.9% (31/33) of human samples were variably positive to those genes. Conclusion: The use of nuc gene based PCR is an accurate and rapid method for S aureus isolates detection. A high prevalence of multiple drug-resistant isolates of S. aureus recovered from both human and milk represents further evidence for possible veterinary hazards as well as public hazards, especially to those that consume milk from this region.

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High Prevalence and Properties of Enterotoxin-ProducingStaphylococcus aureusST5 Strains of Food Sources in China

Cited by 7 publications

References 25 publications

Antibiogram and phylogenetic diversity of enterotoxigenic Staphylococcus aureus strains from milk products and public health implications

Antibiogram and phylogenetic diversity of enterotoxigenic Staphylococcus aureus strains from milk products and public health implications

Staphylococcus aureus in Some Brazilian Dairy Industries: Changes of Contamination and Diversity

Molecular detection of Staphylococcus aureus enterotoxin genes isolated from mastitic milk and humans in El-Behira, Egypt

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