High-Pressure Liquid Chromatographic Determination of Vitamin D3 in Resins, Oils, Dry Concentrates, and Dry Concentrates Containing Vitamin A

Abstract

A high-pressure liquid chromatographic (HPLC) procedure has been developed for the determination of vitamin D3 in resins, oils, dry concentrates, and dry concentrates containing vitamin A. Specificity of the method for vitamin D3 in the presence of isomers and other related constituents was shown by ultimate recovery of the vitamin D3 and the individual constituents and their characterization by other methods such as ultraviolet spectrophotometry. Precision and accuracy are within 2%, and as many as 20 determinations may be completed in a working day. Excellent agreement with the official AOAC biological assay was found. A comparison of the response of isomers of vitamin D3 by the HPLC method vs. other instrumental and chemical procedures shows HPLC to be the most specific method for determining the bioactive isomers.

Effects of supplemental vitamin D3 on serum 25-hydroxycholecalciferol and growth of preweaning and nursery pigs1,2

“…The solution also contained antioxidant ingredients (tertiary butylhydroquinone and citric acid) and an antifoaming ingredient (dimethylpolysiloxane). Confirmation of vitamin D 3 concentrations were performed using an HPLC technique (Hofsass et al, 1976). Dosage treatments were 1) a control 1 mL oral dosage with no supplemental vitamin D 3 , 2) 1 mL of treatment 1 but containing 40,000 IU vitamin D 3 , or 3) 1 mL of treatment 1 but containing 80,000 IU vitamin D 3 .…”

Comprehensive analysis of vitamin D3 impurities in oily drug products using supercritical fluid chromatography–mass spectrometry

“…Very few papers described the analysis of these compounds. The isobaric impurities have been mainly studied qualitatively in the context of degradation studies or to ensure method selectivity in the determination of vitamin D3 11,[13][14][15][16][17][18] . Because it is an analogue to vitamin D, some studies have described the determination of dihydrotachysterol in biological matrices to determine the antirachitic activity, its metabolization and/or bioavailability 19,20 .…”

“…The isobaric impurities have been mainly studied qualitatively in the context of degradation studies or to ensure method selectivity in the determination of vitamin D 3 . 11,[13][14][15][16][17][18] Because it is an analog to vitamin D, some studies have described the determination of dihydrotachysterol in biological matrices to determine the antirachitic activity, its metabolization, and/or bioavailability. 19,20 For octanoate and decanoate ester impurities, Ballard et al 12 described their detection in a degradation study of an experimental tablet formulation.…”

Simultaneous determination of vitamins A, D2 or D3, E and K1 in infant formulas and dairy products by reversed-phase liquid chromatography

“…(1) This reaction was selected for use in the measurement of Fe3+ partially on the basis of studies by Hamilton et al (9,10). The authors found that the role of Fe3+ was that of a catalyst, with over 20 molecules of product produced per Fe3+ ion present.…”