High-Precision Automated Micromanipulation and Adhesive Microbonding With Cantilevered Micropipette Probes in the Dynamic Probing Mode

Xie, Hui; Zhang, Hao; Song, Jian; Meng, Xianghe; Yan, Wen; Sun, Lining

doi:10.1109/tmech.2018.2816957

Cited by 44 publications

(15 citation statements)

References 46 publications

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“…Prior to the experiment, the sensitivity of the probe was well calibrated by the pull-off test on the substrate. 39 Before immediately contacting the cells, the magnetic field was used to measure the resonant frequency of the probe in the liquid and the probe was controlled to maintain an amplitude of 5 nm at this resonant frequency, while the experimental loading speed was set to 0.08 μm/s. After removing the cells from the incubator, the measurement of the cells in each dish was completed within 2 h. On average, each dish can measure about 10 cells, and our observations and other reports indicate that the cells remain viable during this period.…”

Section: ■ Methods and Materialsmentioning

confidence: 99%

Probing Multidimensional Mechanical Phenotyping of Intracellular Structures by Viscoelastic Spectroscopy

Song

Meng

Zhang

et al. 2019

ACS Appl. Mater. Interfaces

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Mechanical phenotyping of complex cellular structures gives insight into the process and function of mechanotransduction in biological systems. Several methods have been developed to characterize intracellular elastic moduli, while direct viscoelastic characterization of intracellular structures is still challenging. Here, we develop a needle tip viscoelastic spectroscopy method to probe multidimensional mechanical phenotyping of intracellular structures during a mini-invasive penetrating process. Viscoelastic spectroscopy is determined by magnetically driven resonant vibration (about 15 kHz) with a tiny amplitude. It not only detects the unique dynamic stiffness, damping, and loss tangent of the cell membrane− cytoskeleton and nucleus−nuclear lamina but also bridges viscoelastic parameters between the mitotic phase and interphase. Self-defined dynamic mechanical ratios of these two phases can identify two malignant cervical cancer cell lines (HeLa-HPV18+, SiHa-HPV16+) whose membrane or nucleus elastic moduli are indistinguishable. This technique provides a quantitative method for studying mechanosensation, mechanotransduction, and mechanoresponse of intracellular structures from a dynamic mechanical perspective. This technique has the potential to become a reliable quantitative measurement method for dynamic mechanical studies of intracellular structures.

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Section: ■ Methods and Materialsmentioning

confidence: 99%

Probing Multidimensional Mechanical Phenotyping of Intracellular Structures by Viscoelastic Spectroscopy

Song

Meng

Zhang

et al. 2019

ACS Appl. Mater. Interfaces

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“…As shown in Fig. 2, the proposed composite probe was fabricated on a high-precision micromanipulation system with a cantilevered micropipette probe (CMP) [32]. This system mainly consists of two optical microscopes (top view and side view), three holders, an AFM control system (an oscillation controller system, a PSD and a laser) and a pneumatic system (which enables positive and negative pressure switching at the CMP end, not shown here).…”

Section: B Microassembly Systemmentioning

confidence: 99%

“…Two nanopositioning stages (NS I and NS II, providing subnanometer motion during assembly) precisely control the interaction between the polymer microcantilever and the CMP tip or the silicon tip based on the feedback signals acquired by the oscillation controller system. The more detailed descriptions can be found in our previous research [32].…”

Section: B Microassembly Systemmentioning

confidence: 99%

High-Bandwidth Multiparametric Kelvin Probe Force Microscopy With Polymer Microcantilevers

et al. 2019

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Simultaneous and rapid measurement of the surface potential (SP) and nanomechanical properties (NMPs) of materials plays an important role in the study of, for example, piezoelectric materials and multi-component composites. In our previous study, a multiparametric Kelvin probe force microscopy (MP-KPFM) was developed to simultaneously measure SP and NMPs using traditional silicon probes. However, its temporal resolution is severely limited by the lower mechanical bandwidth of traditional silicon probes. Here, a composite atomic force microscope (AFM) probe capable of effectively increasing the measurement rate of MP-KPFM was developed. The proposed composite probe consisting of a polymer microcantilever and a silicon tip was fabricated using photolithography and microassembly techniques. Compared to the traditional silicon probe with a similar stiffness, its mechanical bandwidth is increased by about 4 times, which enables fast measurement by implementing the MP-KPFM at higher peak force drive frequencies. Experimental results show that the composite probe's peak force drive frequency is up to 4 kHz, whereas the traditional silicon probe is limited to about 1 kHz. Multiparametric mapping results of a polymer grating demonstrate the capability of the composite probe in fast and simultaneous measurement of SP and NMPs. The proposed composite probe has excellent compatibility and scalability due to the combination of the high mechanical bandwidth of the polymer microcantilever and the rigidity of the silicon tip, which provides a new idea for the development of multifunctional AFM probes.INDEX TERMS Kelvin probe force microscopy, polymer microcantilever, high-bandwidth, surface potential, nanomechanical properties.

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“…Depending on the specific context of applications and their corresponding precision requirements, the tethered [ 8 , 9 , 10 ] or untethered [ 11 , 12 , 13 ] mode of micromanipulation may be employed. With growing needs towards dexterous micromanipulation, the control of contact forces is one of the major issues to tackle.…”

Section: Introductionmentioning

confidence: 99%

A Two-Axis Piezoresistive Force Sensing Tool for Microgripping

Tiwari

Billot

Clevy

et al. 2021

Sensors

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Force sensing has always been an important necessity in making decisions for manipulation. It becomes more appealing in the micro-scale context, especially where the surface forces become predominant. In addition, the deformations happening at the very local level are often coupled, and therefore providing multi-axis force sensing capabilities to microgripper becomes an important necessity. The manufacturing of a multi-axis instrumented microgripper comprises several levels of complexity, especially when it comes to the single wafer fabrication of a sensing and actuation mechanism. To address these requirements, in this work, an instrumented two-axis force sensing tool is proposed, which can then be integrated with the appropriate actuators for microgripping. Indeed, based on the task, the gripper design and shape requirements may differ. To cover wide needs, a versatile manufacturing strategy comprising of the separate fabrication of the passive and sensing parts was especially investigated. At the microscale, signal processing brings additional challenges, especially when we are dealing with multi-axis sensing. Therefore, a proper device, with efficient and appropriate systems and signal processing integration, is highly important. To keep these requirements in consideration, a dedicated clean-room based micro-fabrication of the devices and corresponding electronics to effectively process the signals are presented in this work. The fabricated sensing part can be assembled with wide varieties of passive parts to have different sensing tools as well as grippers. This force sensing tool is based upon the piezoresistive principle, and is experimentally demonstrated with a sensing capability up to 9 mN along the two axes with a resolution of 20 μN. The experimental results validate the measurement error within 1%. This work explains the system design, its working principle, FEM analysis, its fabrication and assembly, followed by the experimental validation of its performance. Moreover, the use of the proposed sensing tool for an instrumented gripper was also discussed and demonstrated with a micrograsping and release task.

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High-Precision Automated Micromanipulation and Adhesive Microbonding With Cantilevered Micropipette Probes in the Dynamic Probing Mode

Cited by 44 publications

References 46 publications

Probing Multidimensional Mechanical Phenotyping of Intracellular Structures by Viscoelastic Spectroscopy

Probing Multidimensional Mechanical Phenotyping of Intracellular Structures by Viscoelastic Spectroscopy

High-Bandwidth Multiparametric Kelvin Probe Force Microscopy With Polymer Microcantilevers

A Two-Axis Piezoresistive Force Sensing Tool for Microgripping

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