High peroxidase activity and stable changes in the cell wall are related to dichlobenil tolerance

García‐Angulo, Penélope; Alonso‐Simón, Ana; Mélida, Hugo; Encina, Antonio; Acebes, José Luis; Álvarez, Jesús Miguel Muñoz

doi:10.1016/j.jplph.2009.01.014

Cited by 21 publications

(32 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This finding is consistent with previous results in which no CAT activity was detected in non-habituated and dichlobenil-habituated bean cells (García-Angulo et al, 2009). One possible explanation could be the plant material used, as heterotrophic calluses, grown under dark conditions have been reported to have a lower CAT activity than plant tissues (Kim et al, 2004).…”

Section: Discussionsupporting

confidence: 93%

“…Interestingly, same results have already been reported for bean cultured cells habituated to diclobenil (García-Angulo et al, 2009). According to our results, the increment in CIII-POX activity measured in NH+10 cells did not seemed to have the capacity to cope with quinclorac-induced oxidative stress, since NH+10 cells had the highest lipid peroxidation levels (Fig.…”

Section: Discussionsupporting

confidence: 86%

“…These results allow us to suggest that the increase in antioxidant activities that putatively confers habituated cells with the capacity to cope with quinclorac is stable and independent of the presence of quinclorac in the culture medium. Interestingly, an acquired high POX activity has also been found in dichlobenil-dehabituated bean calluses grown in a medium lacking the inhibitor (García-Angulo et al, 2009).…”

Section: Discussionmentioning

confidence: 87%

“…The exposure of plant cells to a variety of abiotic stresses such as heavy metals (Paradiso et al, 2008), organochlorines San Miguel et al, 2012) and herbicides (Geoffroy et al, 2004;García-Angulo et al, 2009) often unbalances ROS production and scavenging, leading to oxidative stress (Apel and Hirt, 2004;Gill and Tuteja, 2010). Excessive ROS production can damage cellular components such as proteins, lipids, DNA and polysaccharides, which interrupts normal metabolism (Dat et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…This is the case of bean cell cultures where habituation to dichlobenil is associated with high class III-per (García-Angulo et al, 2009). In the case of maize cells, an increased antioxidant capacity seems to take part in changes associated to the incipient dichlobenil-habituation process (Largo-Gosens et al, 2016), however, antioxidant activities are not implicated in the long-term habituation to high dichlobenil concentrations (Mélida et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Quinclorac-habituation of bean ( Phaseolus vulgaris ) cultured cells is related to an increase in their antioxidant capacity

Largo-Gosens

Castro

Alonso‐Simón

et al. 2016

Plant Physiology and Biochemistry

Self Cite

View full text Add to dashboard Cite

The habituation of bean cells to quinclorac did not rely on cell wall modifications, contrary to what it was previously observed for the well-known cellulose biosynthesis inhibitors dichlobenil or isoxaben. The aim of the present study was to investigate whether or not the bean cells habituation to quinclorac is related to an enhancement of antioxidant activities involved in the scavenging capacity of reactive oxygen species. Treating non-habituated bean calluses with 10 μM quinclorac reduced the relative growth rate and induced a two-fold increase in lipid peroxidation. However, the exposition of quinclorac-habituated cells to a concentration of quinclorac up to 30 μM neither affected their growth rate nor increased their lipid peroxidation levels. Quinclorac-habituated calluses had significantly higher constitutive levels of three antioxidant activities (class-III peroxidase, glutathione reductase, and superoxide dismutase) than those observed in non-habituated calluses, and the treatment of habituated calluses with 30 μM quinclorac significantly increased the level of class III-peroxidase and superoxide dismutase. The results reported here indicate that the process of habituation to quinclorac in bean callus-cultured cells is related, at least partially, to the development of a stable antioxidant capacity that enables them to cope with the oxidative stress caused by quinclorac. Class-III peroxidase and superoxide dismutase activities could play a major role in the quinclorac-habituation. Changes in the antioxidant status of bean cells were stable, since the increase in the antioxidant activities were maintained in quinclorac-dehabituated cells.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Quinclorac-habituation of bean ( Phaseolus vulgaris ) cultured cells is related to an increase in their antioxidant capacity

Largo-Gosens

Castro

Alonso‐Simón

et al. 2016

Plant Physiology and Biochemistry

Self Cite

View full text Add to dashboard Cite

show abstract

Purification and characterization of a soluble β-1,4-glucan from bean (Phaseolus vulgaris L.)-cultured cells dehabituated to dichlobenil

Alonso‐Simón

Encina

Seyama

et al. 2013

Planta

View full text Add to dashboard Cite

Bean cells habituated to grow in the presence of dichlobenil exhibited reduced cellulose and hemicellulose content and an increase in pectic polysaccharides. Furthermore, following the extraction of pectins and hemicelluloses, a large amount of neutral sugars was released. These sugars were found to be part of a soluble β-1,4-glucan in a preliminary characterization, as reported by Encina et al. (Physiol Plant 114:182-191, 2002). When habituated cells were subcultured in the absence of the herbicide (dehabituated cells), the release of neutral sugars after the extraction of pectins and hemicelluloses was maintained. In this study, we have isolated a soluble β-1,4-glucan from dehabituated cells by sonication of the wall residue (cellulose fraction) remaining after fractionation. Gel filtration chromatography revealed that its average molecular size was 14 kDa. Digestion of the sample with endocellulase revealed the presence of cellobiose, cellotriose, and cellotetraose. Methylation analysis showed that 4-linked glucose was the most abundant sugar residue, but 4,6-linked glucose, terminal arabinose and 4-linked galactose for xyloglucan, and arabinogalactan were also identified. NMR analysis showed that this 1,4-glucan may be composed of various kinds of substitutions along the glucan backbone together with acetyl groups linked to the OH group of sugar residues. Thus, despite its relatively high molecular mass, the β-glucan remains soluble because of its unique configuration. This is the first time that a glucan with such characteristics has been isolated and described. The discovery of new molecules, as this β-glucan with unique features, may help understand the composition and arrangement of the polymers within plant cell walls, contributing to a better understanding of this complex structure.

show abstract

Citral Induces Auxin and Ethylene-Mediated Malformations and Arrests Cell Division in Arabidopsis thaliana Roots

et al. 2013

View full text Add to dashboard Cite

High peroxidase activity and stable changes in the cell wall are related to dichlobenil tolerance

Cited by 21 publications

References 34 publications

Quinclorac-habituation of bean ( Phaseolus vulgaris ) cultured cells is related to an increase in their antioxidant capacity

Quinclorac-habituation of bean ( Phaseolus vulgaris ) cultured cells is related to an increase in their antioxidant capacity

Purification and characterization of a soluble β-1,4-glucan from bean (Phaseolus vulgaris L.)-cultured cells dehabituated to dichlobenil

Citral Induces Auxin and Ethylene-Mediated Malformations and Arrests Cell Division in Arabidopsis thaliana Roots

Contact Info

Product

Resources

About