High-performance thin-layer chromatographic method for estimation of rutin in medicinal plants

Kumar, V. Prashanth; Ravishankara, M. N.; Padh, Harish; Rajani, M.

doi:10.1556/jpc.16.2003.5.11

Cited by 14 publications

(4 citation statements)

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“…TLC and HPTLC analysis revealed the occurrence of quercetin and isoquercetin in the dried aqueous extract of L. reticulata whole plant [ 36 ]. A simple HPTLC method was developed to identify alpha-amyrin, beta-sitosterol, lupeol, and n-triacontane from the methanolic extracts of L. reticulata whole plant [ 47 ]. Likewise, a simple and precise HPLC method was developed and validated to quantify p -coumaric acid in L. reticulata extract [ 43 ].…”

Section: Phytochemistrymentioning

confidence: 99%

“…In another study, the presence of polyphenols such as rutin, quercetin and p -coumaric acid were identified in the ethyl acetate extract of L. reticulata leaves by using HPLC method [ 46 ]. Likewise, Prashanth et al [ 47 ], developed and validated a sensitive and accurate HPTLC method to estimate rutin content in L. reticulata leaves. Using spectral (UV, IR, 1 H-NMR, 13 C-NMR and FAB-MS) studies, lupeol (a pentacyclic triterpenoid) was isolated from the chloroform extract of L. reticulata roots [ 48 ].…”

Section: Phytochemistrymentioning

confidence: 99%

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Leptadenia reticulata (Retz.) Wight & Arn. (Jivanti): Botanical, Agronomical, Phytochemical, Pharmacological, and Biotechnological Aspects

et al. 2017

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Leptadenia reticulata (Retz.) Wight & Arn. (Apocynaceae), is a traditional medicinal plant species widely used to treat various ailments such as tuberculosis, hematopoiesis, emaciation, cough, dyspnea, fever, burning sensation, night blindness, cancer, and dysentery. In Ayurveda, it is known for its revitalizing, rejuvenating, and lactogenic properties. This plant is one of the major ingredients in many commercial herbal formulations, including Speman, Envirocare, Calshakti, Antisept, and Chyawanprash. The therapeutic potential of this herb is because of the presence of diverse bioactive compounds such as α-amyrin, β-amyrin, ferulic acid, luteolin, diosmetin, rutin, β-sitosterol, stigmasterol, hentricontanol, a triterpene alcohol simiarenol, apigenin, reticulin, deniculatin, and leptaculatin. However, most biological studies on L. reticulata are restricted to crude extracts, and many biologically active compounds are yet to be identified in order to base the traditional uses of L. reticulata on evidence-based data. At present, L. reticulata is a threatened endangered plant because of overexploitation, unscientific harvesting, and habitat loss. The increased demand from pharmaceutical, nutraceutical, and veterinary industries has prompted its large-scale propagation. However, its commercial cultivation is hampered because of the non-availability of genuine planting material and the lack of knowledge about its agronomical practices. In this regard, micropropagation techniques will be useful to obtain true-to-type L. reticulata planting materials from an elite germplasm to meet the current demand. Adopting other biotechnological approaches such as synthetic seed technology, cryopreservation, cell culture, and genetic transformation can help conservation as well as increased metabolite production from L. reticulata. The present review summarizes scientific information on the botanical, agronomical, phytochemical, pharmacological, and biotechnological aspects of L. reticulata. This comprehensive information will certainly allow better utilization of this industrially important herb towards the discovery of lead drug molecules.

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Section: Phytochemistrymentioning

confidence: 99%

Section: Phytochemistrymentioning

confidence: 99%

Leptadenia reticulata (Retz.) Wight & Arn. (Jivanti): Botanical, Agronomical, Phytochemical, Pharmacological, and Biotechnological Aspects

et al. 2017

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“…In another study, the presence of polyphenols such as rutin, quercetin and p-coumaric acid were identified in the ethyl acetate extract of L. reticulata leaves by using HPLC method [51]. Likewise, Prashanth et al, [52] developed and validated a sensitive and accurate HPTLC method to estimate rutin content in L. reticulata leaves. Using the spectral (UV, IR, 1H NMR, 13CNMR and FAB-MS) studies, lupeol (a pentacyclic triterpenoid) was isolated from the chloroform extract of L. reticulata roots [53].…”

Section: Phytochemistrymentioning

confidence: 99%

“…Hence, it is often confused with other plant species and thus prone to adulteration in the Indian market by other cheap plant powdered materials. A sensitive HPTLC method was to estimate the content of rutin, stigmasterol, and dl-α-tocopherol acetate in L. reticulata leaves [108,109]. They have suggested that it can be used as a marker for identifying L. reticulata precisely for quality analysis regularly.…”

Section: Adulteration and Authentication Of L Reticulatamentioning

confidence: 99%

<em>Leptadenia reticulata</em> (Retz.) Wight (Jivanti): Botanical, Agronomical, Phytochemical, Pharmacological, and Biotechnological Aspects

Mohanty¹,

Swamy²,

Sinniah³

et al. 2017

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Leptadenia reticulata (Ritz.) Wight (Asclepiadaceae), a traditional medicinal plant species, is widely used in traditional medicine to treat various ailments such as tuberculosis, hematopoiesis, emaciation, cough, dyspnea, fever, burning sensation, night blindness, cancer, and dysentery. In Ayurveda, it is known for its revitalizing, rejuvenating, and lactogenic properties. This plant is one of the major ingredients in many commercial herbal formulations, including Speman, Envirocare, Calshakti, Antisept, and Chyawanprash. The therapeutic potential of this herb is because of the presence of diverse bioactive compounds such as α-amyrin, β-amyrin, ferulic acid, luteolin, diosmetin, rutin, β-sitosterol, stigmasterol, hentriacontanol, a triterpene alcohol simiarenol, apigenin, reticulin, deniculatin, and leptaculatin. However, most biological studies on L. reticulata are restricted to crude extracts, and many biologically active compounds are yet to be identified in order to claim the traditional uses of L. reticulata into evidence-based uses. At present, L. reticulata is a threatened endangered plant because of overexploitation, unscientific harvesting, and habitat loss. The increased demand from pharmaceutical, nutraceutical, and veterinary industries has prompted its large-scale propagation. However, its commercial cultivation is hampered because of the non-availability of genuine planting material and the lack of knowledge on its agronomical practices. In this regard, micropropagation technique will be useful to obtain true-to-type L. reticulata planting materials from an elite germplasm to meet the current demand. Adopting other biotechnological approaches such as synthetic seed technology, cryopreservation, cell culture, and genetic transformation can warrant conservation as well as increased metabolite production from L. reticulata. The present review summarizes scientific information on the botanical, agronomical, phytochemical, pharmacological, and biotechnological aspects of L. reticulata. This comprehensive information will certainly allow better utilization of this industrially important herb towards the discovery of lead drug molecules.

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Current Awareness in Phytochemical Analysis

2004

Phytochemical Analysis

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In order to keep subscribers up‐to‐date with the latest developments in their field, John Wiley &Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of phytochemical analysis. Each bibliography is divided into 13 sections: 1 Books, Reviews &Symposia; 2 General; 3 Nucleic Acids; 4 Amino Acids, Proteins &Enzymes; 5 Carbohydrates; 6 Lipids; 7 Secondary Products; 8 Growth Regulators; 9 Industrially‐Important Products; 10 Toxins/Allergens; 11 Pigments; 12 Vitamins; 13 Others. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted.

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High-performance thin-layer chromatographic method for estimation of rutin in medicinal plants

Cited by 14 publications

References 16 publications

Leptadenia reticulata (Retz.) Wight & Arn. (Jivanti): Botanical, Agronomical, Phytochemical, Pharmacological, and Biotechnological Aspects

Leptadenia reticulata (Retz.) Wight & Arn. (Jivanti): Botanical, Agronomical, Phytochemical, Pharmacological, and Biotechnological Aspects

<em>Leptadenia reticulata</em> (Retz.) Wight (Jivanti): Botanical, Agronomical, Phytochemical, Pharmacological, and Biotechnological Aspects

Current Awareness in Phytochemical Analysis

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