High-performance multiplex microvalves fabrication and using for tumor cells staining on a microfluidic chip

Hong, Shao-Li; Tang, Man; Chen, Zhengqi; Ai, Zhao; Liu, Feng; Wang, Shuibing; Zhang, Nangang; Liu, Kan

doi:10.1007/s10544-019-0434-5

Cited by 7 publications

(3 citation statements)

References 40 publications

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“…Much of such literature focuses on microfluidic mixers being used to mix the stain (or fluorescent antibodies etc.) with the cells followed by incubation to achieve the staining 25–29 . Such devices enable low‐volume samples to be efficiently stained with minimal reagents.…”

Section: Slide Smear Preparation and Associated Instrumentationmentioning

confidence: 99%

Recent technical advances in whole slide imaging instrumentation

Katare

Gorthi

2021

Journal of Microscopy

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Microscopic observation of biological specimen smears is the mainstay of diagnostic pathology, as defined by the Digital Pathology Association. Though automated systems for this are commercially available, their bulky size and high cost renders them unusable for remote areas. The research community is investing much effort towards building equivalent but portable, low‐cost systems. An overview of such research is presented here, including a comparative analysis of recent reports. This paper also reviews recently reported systems for automated staining and smear formation, including microfluidic devices; and optical and computational automated microscopy systems including smartphone‐based devices. Image pre‐processing and analysis methods for automated diagnosis are also briefly discussed. It concludes with a set of foreseeable research directions that could lead to affordable, integrated and accurate whole slide imaging systems.

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Section: Slide Smear Preparation and Associated Instrumentationmentioning

confidence: 99%

Recent technical advances in whole slide imaging instrumentation

Katare

Gorthi

2021

Journal of Microscopy

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“…The multiplex microvalves were established by inserting a polydimethylsiloxane (PDMS) membrane into two glasses, which are cheap and simple without any complex silicon-based device or soft lithography. The detailed fabrication and characterization of multiplex valves were reported in our previous work [35]. After the magneticbased immunoassay with multiplex microvalves controlling, IMBstagged influenza HA was flowed into the microchannel of separation zone at a rate of 5 μL/min.…”

Section: Automated Detection Platformmentioning

confidence: 99%

Simultaneous and automated detection of influenza A virus hemagglutinin H7 and H9 based on magnetism and size mediated microfluidic chip

Wang

Zhang

et al. 2020

Sensors and Actuators B: Chemical

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A B S T R A C TInfluenza viruses with multiple subtypes have highly virulent in humans, of which influenza hemagglutinin (HA) is the major viral surface antigen. Simultaneous and automated detection of multiple influenza HA are of great importance for early-stage diagnosis and operator protection. Herein, a magnetism and size mediated microfluidic platform was developed for point-of-care detection of multiple influenza HA. With multiplex microvalves and computer program control, the detection process showed high automation which had a great potential for avoiding the high-risk virus exposure to the operator. Taking advantage of magnetism and size mediated multiple physical fields, multiple influenza HA could be simultaneous separation and detection depended on different-size magnetic beads. Using high-luminance quantum dots as reporter, this assay achieved high sensitivity with a detection limit of 3.4 ng/mL for H7N9 HA and 4.5 ng/mL for H9N2 HA, and showed excellent specificity, anti-interference ability and good reproducibility. These results indicate that this method may propose new avenues for early detection of multiple influenza subtypes.

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“…Microfluidic chips [1] with microchannel, micropump, microvalve, and other units have been widely applied [2] to DNA analysis, immunoassay, and so on, owing to the main feature of rapidity, high efficiency, and low consumption [3,4]. Among them, a mixing microfluidic chip [5] is utilized to realize the functions including rapid mixing and analysis.…”

Section: Introductionmentioning

confidence: 99%

A Rapid Bonding Method for Fabricating Mixing Microfluidic Chip Based on Micromachining Technology

WangJunyao

HouQi

LiuHuan

et al. 2020

Journal of Nanomaterials

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In this paper, a rapid bonding method is reported to fabricate a mixing microfluidic chip. The chip is composed of a glass upper plat and a polyurethane bottom plat. The upper plat with a microchannel is obtained through micromachining technology. And the bottom plat is manufactured via mixing the solution with the component of isocyanic acid and polyether polyol. The influence of the solution’s proportion on the contact angle and bonding force is investigated to enhance the hydrophilicity and the bonding strength. Furthermore, a mixing experiment is implemented to verify the chip’s bonding effect. The experimental results demonstrate that with increasing the proportions of isocyanic acid and polyether polyol, the contact angle and the bonding force increase at first and then decrease gradually. That is attributed to the variation of internal porous structure for different proportions. Considering the contact angle and the bonding force synthetically, the optimal proportion of 3.7 w/w is confirmed. The mixing efficiency is increased from 0.157 to 0.824. Compared with other bonding methods, the method in this paper has the advantages of high efficiency and high bonding strength.

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High-performance multiplex microvalves fabrication and using for tumor cells staining on a microfluidic chip

Cited by 7 publications

References 40 publications

Recent technical advances in whole slide imaging instrumentation

Recent technical advances in whole slide imaging instrumentation

Simultaneous and automated detection of influenza A virus hemagglutinin H7 and H9 based on magnetism and size mediated microfluidic chip

A Rapid Bonding Method for Fabricating Mixing Microfluidic Chip Based on Micromachining Technology

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