High-Performance Liquid Chromatography-Tandem Mass Spectrometry for Buprenorphine Evaluation in Plasma—Application to Pharmacokinetic Studies in Rabbits

Tikhomirov, Marta; Poźniak, Błażej; Śniegocki, Tomasz

doi:10.3390/molecules26020437

Cited by 6 publications

(8 citation statements)

References 41 publications

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“…All parameters, namely declustering potential (DP), collision energy (CE), and entrance potential, were optimized with a direct infusion of working standard solution, as shown in Table 4. The ions selected for the analysis (Table 4) are in good accordance with those reported by other authors [20,26,32,[35][36][37][38][39][40]42,44,45]. These ions were chosen based on the highest intensity, as indicated by mass spectra for FEN (Figure 3).…”

Section: Discussionmentioning

confidence: 52%

“…For the present method, in addition to a standard investigation of the matrix effect [44], we also monitored phospholipids, which served as a marker of lipid-related impurities in plasma. Based on our experience and the reports of other authors [45][46][47], we monitored the presence of 184→184 ion characteristic for this type of compounds. The presented chromatograms (Figure 1, panel C and F) proved that the used mobile phase and appropriate gradient allowed for determining FEN without any influence of phospholipids.…”

Section: Discussionmentioning

confidence: 89%

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Lipemia in the Plasma Sample Affects Fentanyl Measurements by Means of HPLC-MS2 after Liquid-Liquid Extraction

2021

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Examination of fentanyl levels is frequently performed in certain scientific evaluations and forensic toxicology. It often involves the collection of very variable blood samples, including lipemic plasma or serum. To date, many works have reported the methods for fentanyl detection, but none of them have provided information about the impact on the assay performance caused by an excessive amount of lipids. This aspect may be, however, very important for highly lipophilic drugs like fentanyl. To address this issue, we developed the liquid chromatography method with mass spectrometry detection and utilized it to investigate the impact of lipids presence in rabbit plasma on the analytical method performance and validation. The validation procedure, conducted for normal plasma and lipemic plasma separately, resulted in good selectivity, sensitivity and linearity. The limits of detection and quantification were comparable between the two matrices, being slightly lower in normal plasma (0.005 and 0.015 µg/L) than in lipemic plasma (0.008 and 0.020 µg/L). Liquid–liquid extraction provided a low matrix effect regardless of the lipid levels in the samples (<10%), but pronounced differences were found in the recovery and accuracy. In the normal plasma, this parameter was stable and high (around 100%), but in the lipemic matrix, much more variable and less efficient results were obtained. Nevertheless, this difference had no impact on repeatability and reproducibility. In the present work, we provided reliable, convenient and sensitive method for fentanyl detection in the normal and lipemic rabbit plasma. However, construction of two separate validation curves was necessary to provide adequate results since the liquid-liquid extraction was utilized. Therefore, special attention should be paid during fentanyl quantification that involves lipemic plasma samples purified by this technique.

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Section: Discussionmentioning

confidence: 52%

Section: Discussionmentioning

confidence: 89%

Lipemia in the Plasma Sample Affects Fentanyl Measurements by Means of HPLC-MS2 after Liquid-Liquid Extraction

2021

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“…Validation . The method was developed according to the International Council for Harmonisation of Pharmaceutical Requirements for Human Use (ICH) Q2 (R1) methodology and similarly to previously described methods ( 6 , 12 , 14 , 17 , 18 , 25 , 26 ). The following validation parameters were estimated: selectivity, limit of detection (LOD), limit of quantification (LOQ), working range, repeatability, reproducibility, matrix effect and uncertainty of the method.…”

Section: Methodsmentioning

confidence: 99%

“…The LOD and LOQ were estimated by calculations based on the signal-to-noise ratio, which was determined by comparing the measured signals from samples with known low concentrations of analyte with those from blank samples and establishing the minimum concentration at which the analyte could be reliably detected or quantified. A typical signal-to-noise ratio is 3:1 for LOD and 10:1 for LOQ ( 6 , 8 , 14 , 17 , 25 , 31 ). Spiked blank samples were prepared as follows: standard solutions concentrations of 0.2, 1.0, 5.0, 20.0, 50.0 μg g −1 and internal standard (fipronil– 13C 4 ) solutions at 5.0 μg g −1 were added to 5.0 g of sample.…”

Section: Methodsmentioning

confidence: 99%

Liquid chromatography–mass spectrometry analysis of carvacrol in chicken tissues

Śniegocki

2022

Journal of Veterinary Research

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Introduction Carvacrol is an essential oil derived from oregano that is used as a natural additive to improve the efficiency of livestock nutrition. Residues of natural additives such as carvacrol should be monitored in food of animal origin to ensure consumer safety. The aim of this study was to appraise the quick, easy, cheap, effective, rugged and safe (QuEChERS) approach coupled with liquid chromatography and mass spectrometry as a means of carvacrol analysis in chicken tissue. Material and Methods A 5 ± 0.05 g portion of plasma, lung, muscle and liver was mixed for 15 min with 5 mL of 1-butanol and 20 mL of water, then centrifuged. A 0.5 mL volume from the top layer was transferred, then 60 mg of octadecylsilane sorbent, 30 mg of primary and secondary amine and 200 mg of MgSO4 were added. The extract was mixed and centrifuged. The top layer was filtered and then transferred to an autosampler vial for analysis by high-performance liquid chromatography–tandem mass spectrometry. Results The limit of detection was calculated at 0.06 μg g−1 and the limit of quantification was 0.2 μg g−1, with relative standard deviation repeatability and reproducibility below <20%. Conclusion The validation results showed that this method could be a good alternative to determination of carvacrol by gas chromatography and is suitable for carvacrol analysis in different matrices.

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“…The details regarding the development and validation of analytical methods for BPN and FTL have already been reported 36 , 37 . In brief, the HPLC-MS 2 method for BPN was developed for a wide concentration range (0.25–2000 ng/ml) and was proved to be linear.…”

Section: Methodsmentioning

confidence: 99%

Predicting the efficacy of opioid sequestration by intravenous lipid emulsion using biologically relevant in vitro models of drug distribution

Tikhomirov

Jajor

Śniegocki

et al. 2022

Sci Rep

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Intravenous lipid emulsions (ILE), among other uses, are utilized in the treatment of poisonings caused by lipophilic substances. The body of evidence regarding the benefits of this treatment is growing but information about opioids-ILE interaction is still very scarce. In this work, the impact of ILE on the distribution of buprenorphine, fentanyl and butorphanol used in various concentrations (100–500 ng/ml) was investigated. Two different in vitro models were used: disposition of the drugs in plasma after ultracentrifugation and distribution into the simulated biophase (cell monolayer of 3T3 fibroblasts or J774.E macrophages). We confirmed the ability of ILE to sequester the three drugs of interest which results in their decrease in the aqueous part of the plasma by 34.2–38.2%, 11.7–28.5% and 6.0–15.5% for buprenorphine, fentanyl and butorphanol, respectively. Moreover, ILE affected the drug distribution to the biophase in vitro, however, in this case the drug concentration in cells decreased by 97.3 ± 3.1%, 28.6 ± 5.4% and 13.0 ± 7.5% for buprenorphine, fentanyl and butorphanol, respectively. The two models revealed notable differences in ILE’s potential for drug sequestration, especially for buprenorphine. Similar, but not as pronounced tendencies were observed for the two other drugs. These discrepancies may result from the difference in protein abundance and resulting drug-protein binding in both systems. Nevertheless, the results obtained with both in vitro models correlated well with the partition coefficient (logP) values for these drugs.

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High-Performance Liquid Chromatography-Tandem Mass Spectrometry for Buprenorphine Evaluation in Plasma—Application to Pharmacokinetic Studies in Rabbits

Cited by 6 publications

References 41 publications

Lipemia in the Plasma Sample Affects Fentanyl Measurements by Means of HPLC-MS2 after Liquid-Liquid Extraction

Lipemia in the Plasma Sample Affects Fentanyl Measurements by Means of HPLC-MS2 after Liquid-Liquid Extraction

Liquid chromatography–mass spectrometry analysis of carvacrol in chicken tissues

Predicting the efficacy of opioid sequestration by intravenous lipid emulsion using biologically relevant in vitro models of drug distribution

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