Liquid chromatography–mass spectrometry analysis of carvacrol in chicken tissues

Abstract

Introduction Carvacrol is an essential oil derived from oregano that is used as a natural additive to improve the efficiency of livestock nutrition. Residues of natural additives such as carvacrol should be monitored in food of animal origin to ensure consumer safety. The aim of this study was to appraise the quick, easy, cheap, effective, rugged and safe (QuEChERS) approach coupled with liquid chromatography and mass spectrometry as a means of carvacrol analysis in chicken tissue. Material and Methods A 5 ± 0.05 g portion of plasma, lung, muscle and liver was mixed for 15 min with 5 mL of 1-butanol and 20 mL of water, then centrifuged. A 0.5 mL volume from the top layer was transferred, then 60 mg of octadecylsilane sorbent, 30 mg of primary and secondary amine and 200 mg of MgSO4 were added. The extract was mixed and centrifuged. The top layer was filtered and then transferred to an autosampler vial for analysis by high-performance liquid chromatography–tandem mass spectrometry. Results The limit of detection was calculated at 0.06 μg g−1 and the limit of quantification was 0.2 μg g−1, with relative standard deviation repeatability and reproducibility below <20%. Conclusion The validation results showed that this method could be a good alternative to determination of carvacrol by gas chromatography and is suitable for carvacrol analysis in different matrices.

A preliminary study of the poultry body weight effect of carvacrol in litter and of carvacrol residue in organ tissue of exposed chickens

“…The recoveries calculated based on matrix-matched calibration curves were in the range of 98.9–107.7%. The expanded uncertainty did not exceed 34% ( 25 ).…”

“…The extract was mixed and centrifuged at 2,930 × rcf for 10 min at approximately 0°C. A 0.3 mL volume of the top layer was filtered and then transferred to an autosampler vial for analysis by high-performance liquid chromatography–tandem mass spectrometry ( 25 ). The injection volume was 10 μL.…”

“…The column was equilibrated for 2 min. The column operated at 35°C and the ions were monitored in the multiple reaction monitoring (MRM) mode ( 25 ).…”

“…The aim of this study was to evaluate the effect of the addition of carvacrol to litter on weight gain and evaluate the occurrence of residues in chicken tissues. A method for determining carvacrol in plasma, lung, muscles, and liver tissues was used to detect possible residues ( 25 ).…”

The First Evidence on the Occurrence of Bisphenol Analogues in the Aqueous Humor of Patients Undergoing Cataract Surgery

“…In our study, we propose methanol because it allows better separation than acetonitrile. Additionally, based on our previous experience, a small amount of isopropanol was added because we have observed that the isopropyl alcohol possessing a higher elution strength in RP systems reduces the peak tailing of peaks making them slightly higher [ 73 ].…”