High‐performance liquid chromatography separation characteristics of molecular‐imprinted poly(methacrylic acid) microparticles prepared by suspension polymerization

Abstract

Molecular-imprinted poly(methacrylic acid) was synthesized with a template of retinoic acid to separate retinoid derivatives. The suspension polymerization technique was used to prepare round microparticles for highperformance liquid chromatography (HPLC) packing column materials. The effects of the types and amounts of the dispersing agents and surfactants on the structure and size of the prepared molecular-imprinted-polymer particles were investigated. The separation of retinoic acid from its derivatives was more efficient when the perfluorocarbon dispersing agent was used instead of water, as the latter reduced the binding force between the objective molecules and monomers. HPLC separation features were also affected by the size and distribution of particles loaded in the column. A higher retention volume was obtained for smaller particles with a broader size distribution.

Molecular imprinting science and technology: a survey of the literature for the years 2004-2011

“…Efforts to employ MIPs in assays have extended well beyond the large volume of literature focused upon MIPSE, most notably CE and CEC, and LC . In contrast to the 1990s and early 2000s, the proportion of studies devoted to, or heavily dependent upon, LC has reduced significantly.…”

A novel protocol for the preparation of uniform polymer microspheres with high yields through step polymerization of isophorone diisocyanate

“…There has been an urgent need for large‐scale production of polymer microspheres with uniformed size, because these microspheres are widely used in liquid crystal display,1 enzymes immobilization,2 packing materials for chromatography,3 drug delivery,4 and other emerging areas 5, 6. Conventional methods to prepare these polymer beads include suspension polymerization,7 dispersion polymerization,8 and emulsion polymerization 9. Shirasu porous glass membrane emulsification followed by a post‐polymerization10 is also a known process for this purpose.…”

Microsphere Polymers in Molecular Imprinting: Current and Future Perspectives

“…Substances that play a major role in this method are suspension agents that are mixed with the disperse phase. Certain compounds, such as retinoic acid, were tried by Kim [ 140 ], but require catalysts such as triethylamine.…”